Escherichia coli integration host factor binds specifically to the ends of the insertion sequence IS1 and to its major insertion hot-spot in pBR322
We report here that the ends of IS1 are bound and protected in vitro by the heterodimeric protein integration host factor (IHF). Under identical conditions, RNA polymerase binds to one of these ends (IRL) and protects a region that includes the sequences protected by IHF. Other potential sites withi...
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| Veröffentlicht in: | Journal of molecular biology 1987-05, Vol.195 (2), p.261-272 |
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| container_end_page | 272 |
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| container_title | Journal of molecular biology |
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| creator | GAMAS, P CHANDLER, M. G PRENTKI, P GALAS, D. J |
| description | We report here that the ends of IS1 are bound and protected in vitro by the heterodimeric protein integration host factor (IHF). Under identical conditions, RNA polymerase binds to one of these ends (IRL) and protects a region that includes the sequences protected by IHF. Other potential sites within IS1, identified by their homology to the apparent consensus sequence, are not protected. Footprinting analysis of deletion derivatives of the ends demonstrates a correspondence between the ability of the end sequence to bind IHF and its ability to function as an end in transposition. Nonetheless, some transposition occurs in IHF- cells, indicating that IHF is not an essential component of the transposition apparatus. IHF also binds and protects four closely spaced regions within the major hot-spot for insertion of IS1 in the plasmid pBR322. This striking correlation of hot-spot and IHF-binding sites suggests a possible role for IHF in IS1 insertion specificity. |
| doi_str_mv | 10.1016/0022-2836(87)90648-6 |
| format | Article |
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This striking correlation of hot-spot and IHF-binding sites suggests a possible role for IHF in IS1 insertion specificity.</description><identifier>ISSN: 0022-2836</identifier><identifier>EISSN: 1089-8638</identifier><identifier>DOI: 10.1016/0022-2836(87)90648-6</identifier><identifier>PMID: 2821273</identifier><identifier>CODEN: JMOBAK</identifier><language>eng</language><publisher>Oxford: Elsevier</publisher><subject>Bacterial Proteins - metabolism ; Binding Sites ; Biological and medical sciences ; DNA Transposable Elements ; DNA, Bacterial - metabolism ; DNA-Directed RNA Polymerases - metabolism ; Escherichia coli ; Escherichia coli - genetics ; Fundamental and applied biological sciences. Psychology ; Genic rearrangement. Recombination. Transposable element ; Integration Host Factors ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Plasmids ; Repetitive Sequences, Nucleic Acid ; Transcription, Genetic</subject><ispartof>Journal of molecular biology, 1987-05, Vol.195 (2), p.261-272</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-1da59bf66cc0369ea8c96bcdf612ad89b6c7a859042e36502c4c3cb636c63a763</citedby><cites>FETCH-LOGICAL-c362t-1da59bf66cc0369ea8c96bcdf612ad89b6c7a859042e36502c4c3cb636c63a763</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8244892$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2821273$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>GAMAS, P</creatorcontrib><creatorcontrib>CHANDLER, M. G</creatorcontrib><creatorcontrib>PRENTKI, P</creatorcontrib><creatorcontrib>GALAS, D. J</creatorcontrib><title>Escherichia coli integration host factor binds specifically to the ends of the insertion sequence IS1 and to its major insertion hot-spot in pBR322</title><title>Journal of molecular biology</title><addtitle>J Mol Biol</addtitle><description>We report here that the ends of IS1 are bound and protected in vitro by the heterodimeric protein integration host factor (IHF). Under identical conditions, RNA polymerase binds to one of these ends (IRL) and protects a region that includes the sequences protected by IHF. Other potential sites within IS1, identified by their homology to the apparent consensus sequence, are not protected. Footprinting analysis of deletion derivatives of the ends demonstrates a correspondence between the ability of the end sequence to bind IHF and its ability to function as an end in transposition. Nonetheless, some transposition occurs in IHF- cells, indicating that IHF is not an essential component of the transposition apparatus. IHF also binds and protects four closely spaced regions within the major hot-spot for insertion of IS1 in the plasmid pBR322. This striking correlation of hot-spot and IHF-binding sites suggests a possible role for IHF in IS1 insertion specificity.</description><subject>Bacterial Proteins - metabolism</subject><subject>Binding Sites</subject><subject>Biological and medical sciences</subject><subject>DNA Transposable Elements</subject><subject>DNA, Bacterial - metabolism</subject><subject>DNA-Directed RNA Polymerases - metabolism</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genic rearrangement. Recombination. Transposable element</subject><subject>Integration Host Factors</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Plasmids</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>Transcription, Genetic</subject><issn>0022-2836</issn><issn>1089-8638</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctu1DAUhi0EKtPCG4DkBUKwCPg2J86yVC1UqoTEZW05Jw5xlYmDj2fR5-CFSaajYcnKlv_v_Dryx9grKT5IIeGjEEpVymp4Z-v3jQBjK3jCNlLYprKg7VO2OSHP2TnRvRBiq409Y2fKKqlqvWF_rgmHkCMO0XNMY-RxKuFX9iWmiQ-JCu89lpR5G6eOOM0BYx_Rj-MDL4mXIfCwBqk_3ONEIR9mKfzehwkDv_0uuZ-6lY6F-M7fL23_uCGViuZUlic-f_qmlXrBnvV-pPDyeF6wnzfXP66-VHdfP99eXd5VqEGVSnZ-27Q9AKLQ0ARvsYEWux6k8p1tWsDa220jjAoatkKhQY0taEDQvgZ9wd4-9s45LbtScbtIGMbRTyHtyVkplDBC_BeUppYSpFxA8whiTkQ59G7Ocefzg5PCrdLcasStRpyt3UGaWxd5fezft7vQnYaOlpb8zTH3tPx8n_2EkU6YVcbYRum_qhugJQ</recordid><startdate>19870520</startdate><enddate>19870520</enddate><creator>GAMAS, P</creator><creator>CHANDLER, M. G</creator><creator>PRENTKI, P</creator><creator>GALAS, D. J</creator><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19870520</creationdate><title>Escherichia coli integration host factor binds specifically to the ends of the insertion sequence IS1 and to its major insertion hot-spot in pBR322</title><author>GAMAS, P ; CHANDLER, M. G ; PRENTKI, P ; GALAS, D. 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Transposable element</topic><topic>Integration Host Factors</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Plasmids</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GAMAS, P</creatorcontrib><creatorcontrib>CHANDLER, M. G</creatorcontrib><creatorcontrib>PRENTKI, P</creatorcontrib><creatorcontrib>GALAS, D. 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J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Escherichia coli integration host factor binds specifically to the ends of the insertion sequence IS1 and to its major insertion hot-spot in pBR322</atitle><jtitle>Journal of molecular biology</jtitle><addtitle>J Mol Biol</addtitle><date>1987-05-20</date><risdate>1987</risdate><volume>195</volume><issue>2</issue><spage>261</spage><epage>272</epage><pages>261-272</pages><issn>0022-2836</issn><eissn>1089-8638</eissn><coden>JMOBAK</coden><abstract>We report here that the ends of IS1 are bound and protected in vitro by the heterodimeric protein integration host factor (IHF). Under identical conditions, RNA polymerase binds to one of these ends (IRL) and protects a region that includes the sequences protected by IHF. Other potential sites within IS1, identified by their homology to the apparent consensus sequence, are not protected. Footprinting analysis of deletion derivatives of the ends demonstrates a correspondence between the ability of the end sequence to bind IHF and its ability to function as an end in transposition. Nonetheless, some transposition occurs in IHF- cells, indicating that IHF is not an essential component of the transposition apparatus. IHF also binds and protects four closely spaced regions within the major hot-spot for insertion of IS1 in the plasmid pBR322. This striking correlation of hot-spot and IHF-binding sites suggests a possible role for IHF in IS1 insertion specificity.</abstract><cop>Oxford</cop><pub>Elsevier</pub><pmid>2821273</pmid><doi>10.1016/0022-2836(87)90648-6</doi><tpages>12</tpages></addata></record> |
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| ispartof | Journal of molecular biology, 1987-05, Vol.195 (2), p.261-272 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Bacterial Proteins - metabolism Binding Sites Biological and medical sciences DNA Transposable Elements DNA, Bacterial - metabolism DNA-Directed RNA Polymerases - metabolism Escherichia coli Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genic rearrangement. Recombination. Transposable element Integration Host Factors Molecular and cellular biology Molecular genetics Molecular Sequence Data Plasmids Repetitive Sequences, Nucleic Acid Transcription, Genetic |
| title | Escherichia coli integration host factor binds specifically to the ends of the insertion sequence IS1 and to its major insertion hot-spot in pBR322 |
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