Presence of a unique glycoconjugate on the surface of rat primordial germ cells during migration
This investigation was undertaken to examine the chemical nature of components on the surface of primordial germ cells (PGCs) possibly related to their directed migration during development. To this end, lectins conjugated to horseradish peroxidase were used as specific histochemical probes to chara...
Gespeichert in:
| Veröffentlicht in: | Cell differentiation 1987-08, Vol.21 (3), p.199-211 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 211 |
|---|---|
| container_issue | 3 |
| container_start_page | 199 |
| container_title | Cell differentiation |
| container_volume | 21 |
| creator | Fazel, A.R. Schulte, B.A. Thompson, R.P. Spicer, S.S. |
| description | This investigation was undertaken to examine the chemical nature of components on the surface of primordial germ cells (PGCs) possibly related to their directed migration during development. To this end, lectins conjugated to horseradish peroxidase were used as specific histochemical probes to characterize the structure of PGC cell surface glycoconjugates and changes in their composition during and after their migration in the rat embryo. A lectin specific for terminal
N-acetylgalactosamine (GalNAc) from
Dolichos biflorus intensely stained the cell surface and a perinuclear region assumed to be Golgi zone of PGCs only during their migration. With one exception, no other site in the embryo stained with this lectin as migration proceeded. These observations suggest that the GalNAc-containing glycoconjugates on the surface of PGCs may be of functional importance in regulating the guidance and locomotion of these cells during the course of their extensive migration. |
| doi_str_mv | 10.1016/0045-6039(87)90456-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_81013872</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0045603987904568</els_id><sourcerecordid>14965977</sourcerecordid><originalsourceid>FETCH-LOGICAL-c417t-80a9902dd78ce15624a3196730e2682985ddeed524aeb549fb3b02d2e0452e023</originalsourceid><addsrcrecordid>eNqFkUtLxDAUhbNQfIz-A4UsRHRRzaOPZCPI4AsEXeg6ZpLbmqFtNGkF_72pM8xSNwm557uXe04QOqLkghJaXhKSF1lJuDwT1blMjzITW2hvU95F-zEuCWGEFnIH7fCyYIzme-jtOUCE3gD2NdZ47N3nCLhpv403vl-OjR6S1OPhHXAcQ61XZNAD_giu88E63eIGQocNtG3Edgyub3DnmsQ43x-g7Vq3EQ7X9wy93t68zO-zx6e7h_n1Y2ZyWg2ZIFpKwqythAFalCzXnMqy4gRYKZgUhbUAtkh1WBS5rBd8kXAGyWE6GJ-h09Xcj-CThTiozsVpJd2DH6MSKScuqv9BmsuykFWVwHwFmuBjDFCrybEO34oSNaWupnjVFK8SlfpNXYnUdryePy46sJumdeRJP1nrOhrd1kH3xsUNJpJtwnnCrlYYpNC-HAQVjZs-yroAZlDWu7_3-AHuHZ-i</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>14965977</pqid></control><display><type>article</type><title>Presence of a unique glycoconjugate on the surface of rat primordial germ cells during migration</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Fazel, A.R. ; Schulte, B.A. ; Thompson, R.P. ; Spicer, S.S.</creator><creatorcontrib>Fazel, A.R. ; Schulte, B.A. ; Thompson, R.P. ; Spicer, S.S.</creatorcontrib><description>This investigation was undertaken to examine the chemical nature of components on the surface of primordial germ cells (PGCs) possibly related to their directed migration during development. To this end, lectins conjugated to horseradish peroxidase were used as specific histochemical probes to characterize the structure of PGC cell surface glycoconjugates and changes in their composition during and after their migration in the rat embryo. A lectin specific for terminal
N-acetylgalactosamine (GalNAc) from
Dolichos biflorus intensely stained the cell surface and a perinuclear region assumed to be Golgi zone of PGCs only during their migration. With one exception, no other site in the embryo stained with this lectin as migration proceeded. These observations suggest that the GalNAc-containing glycoconjugates on the surface of PGCs may be of functional importance in regulating the guidance and locomotion of these cells during the course of their extensive migration.</description><identifier>ISSN: 0045-6039</identifier><identifier>DOI: 10.1016/0045-6039(87)90456-8</identifier><identifier>PMID: 3652214</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Cell migration ; Cell Movement ; Embryogenesis ; Embryology: invertebrates and vertebrates. Teratology ; Fundamental and applied biological sciences. Psychology ; Genitalia - embryology ; germ cells ; Germ Cells - cytology ; Germ Cells - metabolism ; Gestational Age ; Glycoconjugates ; Glycoconjugates - metabolism ; Glycoproteins - metabolism ; Indifferent gonad ; Lectin histochemistry ; Organogenesis. Fetal development ; Organogenesis. Physiological fonctions ; Polysaccharides - metabolism ; Primordial germ cells ; Rats ; Receptors, Mitogen - metabolism</subject><ispartof>Cell differentiation, 1987-08, Vol.21 (3), p.199-211</ispartof><rights>1987</rights><rights>1987 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-80a9902dd78ce15624a3196730e2682985ddeed524aeb549fb3b02d2e0452e023</citedby><cites>FETCH-LOGICAL-c417t-80a9902dd78ce15624a3196730e2682985ddeed524aeb549fb3b02d2e0452e023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8319033$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3652214$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fazel, A.R.</creatorcontrib><creatorcontrib>Schulte, B.A.</creatorcontrib><creatorcontrib>Thompson, R.P.</creatorcontrib><creatorcontrib>Spicer, S.S.</creatorcontrib><title>Presence of a unique glycoconjugate on the surface of rat primordial germ cells during migration</title><title>Cell differentiation</title><addtitle>Cell Differ</addtitle><description>This investigation was undertaken to examine the chemical nature of components on the surface of primordial germ cells (PGCs) possibly related to their directed migration during development. To this end, lectins conjugated to horseradish peroxidase were used as specific histochemical probes to characterize the structure of PGC cell surface glycoconjugates and changes in their composition during and after their migration in the rat embryo. A lectin specific for terminal
N-acetylgalactosamine (GalNAc) from
Dolichos biflorus intensely stained the cell surface and a perinuclear region assumed to be Golgi zone of PGCs only during their migration. With one exception, no other site in the embryo stained with this lectin as migration proceeded. These observations suggest that the GalNAc-containing glycoconjugates on the surface of PGCs may be of functional importance in regulating the guidance and locomotion of these cells during the course of their extensive migration.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell migration</subject><subject>Cell Movement</subject><subject>Embryogenesis</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genitalia - embryology</subject><subject>germ cells</subject><subject>Germ Cells - cytology</subject><subject>Germ Cells - metabolism</subject><subject>Gestational Age</subject><subject>Glycoconjugates</subject><subject>Glycoconjugates - metabolism</subject><subject>Glycoproteins - metabolism</subject><subject>Indifferent gonad</subject><subject>Lectin histochemistry</subject><subject>Organogenesis. Fetal development</subject><subject>Organogenesis. Physiological fonctions</subject><subject>Polysaccharides - metabolism</subject><subject>Primordial germ cells</subject><subject>Rats</subject><subject>Receptors, Mitogen - metabolism</subject><issn>0045-6039</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtLxDAUhbNQfIz-A4UsRHRRzaOPZCPI4AsEXeg6ZpLbmqFtNGkF_72pM8xSNwm557uXe04QOqLkghJaXhKSF1lJuDwT1blMjzITW2hvU95F-zEuCWGEFnIH7fCyYIzme-jtOUCE3gD2NdZ47N3nCLhpv403vl-OjR6S1OPhHXAcQ61XZNAD_giu88E63eIGQocNtG3Edgyub3DnmsQ43x-g7Vq3EQ7X9wy93t68zO-zx6e7h_n1Y2ZyWg2ZIFpKwqythAFalCzXnMqy4gRYKZgUhbUAtkh1WBS5rBd8kXAGyWE6GJ-h09Xcj-CThTiozsVpJd2DH6MSKScuqv9BmsuykFWVwHwFmuBjDFCrybEO34oSNaWupnjVFK8SlfpNXYnUdryePy46sJumdeRJP1nrOhrd1kH3xsUNJpJtwnnCrlYYpNC-HAQVjZs-yroAZlDWu7_3-AHuHZ-i</recordid><startdate>19870801</startdate><enddate>19870801</enddate><creator>Fazel, A.R.</creator><creator>Schulte, B.A.</creator><creator>Thompson, R.P.</creator><creator>Spicer, S.S.</creator><general>Elsevier B.V</general><general>North-Holland</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19870801</creationdate><title>Presence of a unique glycoconjugate on the surface of rat primordial germ cells during migration</title><author>Fazel, A.R. ; Schulte, B.A. ; Thompson, R.P. ; Spicer, S.S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-80a9902dd78ce15624a3196730e2682985ddeed524aeb549fb3b02d2e0452e023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell migration</topic><topic>Cell Movement</topic><topic>Embryogenesis</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genitalia - embryology</topic><topic>germ cells</topic><topic>Germ Cells - cytology</topic><topic>Germ Cells - metabolism</topic><topic>Gestational Age</topic><topic>Glycoconjugates</topic><topic>Glycoconjugates - metabolism</topic><topic>Glycoproteins - metabolism</topic><topic>Indifferent gonad</topic><topic>Lectin histochemistry</topic><topic>Organogenesis. Fetal development</topic><topic>Organogenesis. Physiological fonctions</topic><topic>Polysaccharides - metabolism</topic><topic>Primordial germ cells</topic><topic>Rats</topic><topic>Receptors, Mitogen - metabolism</topic><toplevel>online_resources</toplevel><creatorcontrib>Fazel, A.R.</creatorcontrib><creatorcontrib>Schulte, B.A.</creatorcontrib><creatorcontrib>Thompson, R.P.</creatorcontrib><creatorcontrib>Spicer, S.S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell differentiation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fazel, A.R.</au><au>Schulte, B.A.</au><au>Thompson, R.P.</au><au>Spicer, S.S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Presence of a unique glycoconjugate on the surface of rat primordial germ cells during migration</atitle><jtitle>Cell differentiation</jtitle><addtitle>Cell Differ</addtitle><date>1987-08-01</date><risdate>1987</risdate><volume>21</volume><issue>3</issue><spage>199</spage><epage>211</epage><pages>199-211</pages><issn>0045-6039</issn><abstract>This investigation was undertaken to examine the chemical nature of components on the surface of primordial germ cells (PGCs) possibly related to their directed migration during development. To this end, lectins conjugated to horseradish peroxidase were used as specific histochemical probes to characterize the structure of PGC cell surface glycoconjugates and changes in their composition during and after their migration in the rat embryo. A lectin specific for terminal
N-acetylgalactosamine (GalNAc) from
Dolichos biflorus intensely stained the cell surface and a perinuclear region assumed to be Golgi zone of PGCs only during their migration. With one exception, no other site in the embryo stained with this lectin as migration proceeded. These observations suggest that the GalNAc-containing glycoconjugates on the surface of PGCs may be of functional importance in regulating the guidance and locomotion of these cells during the course of their extensive migration.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>3652214</pmid><doi>10.1016/0045-6039(87)90456-8</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0045-6039 |
| ispartof | Cell differentiation, 1987-08, Vol.21 (3), p.199-211 |
| issn | 0045-6039 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81013872 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Biological and medical sciences Cell migration Cell Movement Embryogenesis Embryology: invertebrates and vertebrates. Teratology Fundamental and applied biological sciences. Psychology Genitalia - embryology germ cells Germ Cells - cytology Germ Cells - metabolism Gestational Age Glycoconjugates Glycoconjugates - metabolism Glycoproteins - metabolism Indifferent gonad Lectin histochemistry Organogenesis. Fetal development Organogenesis. Physiological fonctions Polysaccharides - metabolism Primordial germ cells Rats Receptors, Mitogen - metabolism |
| title | Presence of a unique glycoconjugate on the surface of rat primordial germ cells during migration |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T01%3A58%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Presence%20of%20a%20unique%20glycoconjugate%20on%20the%20surface%20of%20rat%20primordial%20germ%20cells%20during%20migration&rft.jtitle=Cell%20differentiation&rft.au=Fazel,%20A.R.&rft.date=1987-08-01&rft.volume=21&rft.issue=3&rft.spage=199&rft.epage=211&rft.pages=199-211&rft.issn=0045-6039&rft_id=info:doi/10.1016/0045-6039(87)90456-8&rft_dat=%3Cproquest_cross%3E14965977%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=14965977&rft_id=info:pmid/3652214&rft_els_id=0045603987904568&rfr_iscdi=true |