Does the degree of polymerization of haptoglobin influence the results of the radial immunodiffusion assay?
The degree of polymerization of haptoglobin (Hp) phenotypes 2-1 and 2-2 was studied. In individual samples the degree of polymerization tends to be positively correlated with the Hp concentration as determined by the radial immunodiffusion technique (RID), while an inverse relationship between the s...
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| Veröffentlicht in: | Clinica chimica acta 1987-06, Vol.165 (2), p.155-163 |
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| container_title | Clinica chimica acta |
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| creator | Van Rijn, H.J.M. Wisman, J.P.W. Meyling, F.Gmelig |
| description | The degree of polymerization of haptoglobin (Hp) phenotypes 2-1 and 2-2 was studied. In individual samples the degree of polymerization tends to be positively correlated with the Hp concentration as determined by the radial immunodiffusion technique (RID), while an inverse relationship between the size of Hp polymers and the Hp concentration in RID was expected.
After reductive cleavage of Hp, the apparent Hp concentration became higher in individual samples with different degrees of polymerization; the shift to higher values appeared to be independent of the degree of polymerization in samples of phenotypes Hp 2-1 and Hp 2-2.
Consequently, the degree of polymerization as such does not have an impact on the outcome of RID at a technical level. |
| doi_str_mv | 10.1016/0009-8981(87)90159-8 |
| format | Article |
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After reductive cleavage of Hp, the apparent Hp concentration became higher in individual samples with different degrees of polymerization; the shift to higher values appeared to be independent of the degree of polymerization in samples of phenotypes Hp 2-1 and Hp 2-2.
Consequently, the degree of polymerization as such does not have an impact on the outcome of RID at a technical level.</description><identifier>ISSN: 0009-8981</identifier><identifier>EISSN: 1873-3492</identifier><identifier>DOI: 10.1016/0009-8981(87)90159-8</identifier><identifier>PMID: 3115627</identifier><identifier>CODEN: CCATAR</identifier><language>eng</language><publisher>Shannon: Elsevier B.V</publisher><subject>Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Electrophoresis, Polyacrylamide Gel ; Fundamental and applied biological sciences. Psychology ; Glycoproteins ; Haptoglobin ; Haptoglobins - analysis ; Humans ; Immunodiffusion ; Indicators and Reagents ; Oxidation-Reduction ; Phenotype ; Polymers ; Proteins ; Radial immunodiffusion</subject><ispartof>Clinica chimica acta, 1987-06, Vol.165 (2), p.155-163</ispartof><rights>1987</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c432t-8d86d3ac85ae115aefe433fe011acd4f747151d23eec804976066b784aefca343</citedby><cites>FETCH-LOGICAL-c432t-8d86d3ac85ae115aefe433fe011acd4f747151d23eec804976066b784aefca343</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0009898187901598$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7490915$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3115627$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Van Rijn, H.J.M.</creatorcontrib><creatorcontrib>Wisman, J.P.W.</creatorcontrib><creatorcontrib>Meyling, F.Gmelig</creatorcontrib><title>Does the degree of polymerization of haptoglobin influence the results of the radial immunodiffusion assay?</title><title>Clinica chimica acta</title><addtitle>Clin Chim Acta</addtitle><description>The degree of polymerization of haptoglobin (Hp) phenotypes 2-1 and 2-2 was studied. In individual samples the degree of polymerization tends to be positively correlated with the Hp concentration as determined by the radial immunodiffusion technique (RID), while an inverse relationship between the size of Hp polymers and the Hp concentration in RID was expected.
After reductive cleavage of Hp, the apparent Hp concentration became higher in individual samples with different degrees of polymerization; the shift to higher values appeared to be independent of the degree of polymerization in samples of phenotypes Hp 2-1 and Hp 2-2.
Consequently, the degree of polymerization as such does not have an impact on the outcome of RID at a technical level.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycoproteins</subject><subject>Haptoglobin</subject><subject>Haptoglobins - analysis</subject><subject>Humans</subject><subject>Immunodiffusion</subject><subject>Indicators and Reagents</subject><subject>Oxidation-Reduction</subject><subject>Phenotype</subject><subject>Polymers</subject><subject>Proteins</subject><subject>Radial immunodiffusion</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1v1DAQhi0EareFf9BKOSAEhxQ7dmLnAkItX1KlXuBsee1x69aJt54Eafn1OLurPXKyXs_zjkYPIReMXjHKuo-U0r5WvWLvlfzQU9aW9IKsmJK85qJvXpLVETklZ4iPJQrasRNywhlru0auyNNNAqymB6gc3GeAKvlqk-J2gBz-mimkcfl5MJsp3ce0DmMVRh9nGC3sWhlwjhMu0C4aF0yswjDMY3LB-xmXFQbRbD-_Jq-8iQhvDu85-f3t66_rH_Xt3fef119uayt4M9XKqc5xY1VroJxpwIPg3ANlzFgnvBSStcw1HMAqKnrZ0a5bSyUKaQ0X_Jy82-_d5PQ8A056CGghRjNCmlGroo-1zQKKPWhzQszg9SaHweStZlQvjvUiUC8CtZJ651irUrs87J_XA7hj6SC1zN8e5gatiT6b0QY8YlL0tGdtwT7tMSgu_gTIGm1YxLqQwU7apfD_O_4B1zOZug</recordid><startdate>19870615</startdate><enddate>19870615</enddate><creator>Van Rijn, H.J.M.</creator><creator>Wisman, J.P.W.</creator><creator>Meyling, F.Gmelig</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19870615</creationdate><title>Does the degree of polymerization of haptoglobin influence the results of the radial immunodiffusion assay?</title><author>Van Rijn, H.J.M. ; Wisman, J.P.W. ; Meyling, F.Gmelig</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c432t-8d86d3ac85ae115aefe433fe011acd4f747151d23eec804976066b784aefca343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycoproteins</topic><topic>Haptoglobin</topic><topic>Haptoglobins - analysis</topic><topic>Humans</topic><topic>Immunodiffusion</topic><topic>Indicators and Reagents</topic><topic>Oxidation-Reduction</topic><topic>Phenotype</topic><topic>Polymers</topic><topic>Proteins</topic><topic>Radial immunodiffusion</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Van Rijn, H.J.M.</creatorcontrib><creatorcontrib>Wisman, J.P.W.</creatorcontrib><creatorcontrib>Meyling, F.Gmelig</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Van Rijn, H.J.M.</au><au>Wisman, J.P.W.</au><au>Meyling, F.Gmelig</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Does the degree of polymerization of haptoglobin influence the results of the radial immunodiffusion assay?</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>1987-06-15</date><risdate>1987</risdate><volume>165</volume><issue>2</issue><spage>155</spage><epage>163</epage><pages>155-163</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><coden>CCATAR</coden><abstract>The degree of polymerization of haptoglobin (Hp) phenotypes 2-1 and 2-2 was studied. In individual samples the degree of polymerization tends to be positively correlated with the Hp concentration as determined by the radial immunodiffusion technique (RID), while an inverse relationship between the size of Hp polymers and the Hp concentration in RID was expected.
After reductive cleavage of Hp, the apparent Hp concentration became higher in individual samples with different degrees of polymerization; the shift to higher values appeared to be independent of the degree of polymerization in samples of phenotypes Hp 2-1 and Hp 2-2.
Consequently, the degree of polymerization as such does not have an impact on the outcome of RID at a technical level.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>3115627</pmid><doi>10.1016/0009-8981(87)90159-8</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Clinica chimica acta, 1987-06, Vol.165 (2), p.155-163 |
| issn | 0009-8981 1873-3492 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Analytical, structural and metabolic biochemistry Biological and medical sciences Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Glycoproteins Haptoglobin Haptoglobins - analysis Humans Immunodiffusion Indicators and Reagents Oxidation-Reduction Phenotype Polymers Proteins Radial immunodiffusion |
| title | Does the degree of polymerization of haptoglobin influence the results of the radial immunodiffusion assay? |
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