Interactions in the hydrogen belts of membranes: Cholesterol leaving phosphatidylcholine bilayers
Cholesterol transfer from sonicated liposomes of phosphatidylcholine containing 10 or 30 mole percent cholesterol was measured with erythrocytes as acceptor. The activation energies of the (rate-limiting) bilayer-cholesterol dissociation were determined. In parallel experiments, phosphatidylcholine...
Gespeichert in:
| Veröffentlicht in: | Biochemical and biophysical research communications 1984-02, Vol.118 (3), p.743-746 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 746 |
|---|---|
| container_issue | 3 |
| container_start_page | 743 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 118 |
| creator | Ramsammy, L.S. Chauhan, V.P.S. Box, L.L. Brockerhoff, H. |
| description | Cholesterol transfer from sonicated liposomes of phosphatidylcholine containing 10 or 30 mole percent cholesterol was measured with erythrocytes as acceptor. The activation energies of the (rate-limiting) bilayer-cholesterol dissociation were determined. In parallel experiments, phosphatidylcholine was replaced by an analog lacking the carbonyl oxygens, dietherphosphatidylcholine. The activation energies for dissociation of cholesterol from this phospholipid were three Cal/mole smaller than those for cholesterol-phosphatidylcholine dissociation, at both concentrations of cholesterol. These results demonstrate the involvement of the carbonyl oxygen in cholesterol-phospholipid bonding and support the hypothesis of lipid-lipid hydrogen bonding in the hydrogen belts of membranes. |
| doi_str_mv | 10.1016/0006-291X(84)91457-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_81002353</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0006291X84914578</els_id><sourcerecordid>13825136</sourcerecordid><originalsourceid>FETCH-LOGICAL-c388t-9ff87c2f9b0aa7e87fcac165d8cf05256817a58b3c35984678d24e2baeedc5c93</originalsourceid><addsrcrecordid>eNqFUctKxDAUDaKM4-MPFLISXVRv2qRNXQgy-IIBNwruQpre2kjajElHmL-34wwudXUX53EP5xBywuCSAcuvACBP0pK9nUt-UTIuikTukCmDEpKUAd8l01_KPjmI8QOAMZ6XEzLJC-AMxJTop37AoM1gfR-p7enQIm1XdfDv2NMK3RCpb2iHXRV0j_GazlrvMI4i76hD_WX7d7pofVy0erD1ypkRtz3Syjq9whCPyF6jXcTj7T0kr_d3L7PHZP788DS7nScmk3JIyqaRhUmbsgKtC5RFY7RhuailaUCkIpes0EJWmclEKXleyDrlmFYasTbClNkhOdv4LoL_XI4JVWejQefG2H4ZlWQAaSayf4ksk6lgWT4S-YZogo8xYKMWwXY6rBQDtZ5ArftV636V5OpnAiVH2enWf1l1WP-Ktp2P-M0Gx7GNL4tBRWOxN1jbgGZQtbd_P_gG3jeX4Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>13825136</pqid></control><display><type>article</type><title>Interactions in the hydrogen belts of membranes: Cholesterol leaving phosphatidylcholine bilayers</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Ramsammy, L.S. ; Chauhan, V.P.S. ; Box, L.L. ; Brockerhoff, H.</creator><creatorcontrib>Ramsammy, L.S. ; Chauhan, V.P.S. ; Box, L.L. ; Brockerhoff, H.</creatorcontrib><description>Cholesterol transfer from sonicated liposomes of phosphatidylcholine containing 10 or 30 mole percent cholesterol was measured with erythrocytes as acceptor. The activation energies of the (rate-limiting) bilayer-cholesterol dissociation were determined. In parallel experiments, phosphatidylcholine was replaced by an analog lacking the carbonyl oxygens, dietherphosphatidylcholine. The activation energies for dissociation of cholesterol from this phospholipid were three Cal/mole smaller than those for cholesterol-phosphatidylcholine dissociation, at both concentrations of cholesterol. These results demonstrate the involvement of the carbonyl oxygen in cholesterol-phospholipid bonding and support the hypothesis of lipid-lipid hydrogen bonding in the hydrogen belts of membranes.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(84)91457-8</identifier><identifier>PMID: 6704105</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; cholesterol ; Cholesterol - metabolism ; erythrocytes ; Erythrocytes - metabolism ; Hydrogen Bonding ; Lipid Bilayers - metabolism ; liposomes ; Liposomes - metabolism ; membrane structure ; Phosphatidylcholines - metabolism ; Rats ; Thermodynamics</subject><ispartof>Biochemical and biophysical research communications, 1984-02, Vol.118 (3), p.743-746</ispartof><rights>1984</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-9ff87c2f9b0aa7e87fcac165d8cf05256817a58b3c35984678d24e2baeedc5c93</citedby><cites>FETCH-LOGICAL-c388t-9ff87c2f9b0aa7e87fcac165d8cf05256817a58b3c35984678d24e2baeedc5c93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-291X(84)91457-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6704105$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ramsammy, L.S.</creatorcontrib><creatorcontrib>Chauhan, V.P.S.</creatorcontrib><creatorcontrib>Box, L.L.</creatorcontrib><creatorcontrib>Brockerhoff, H.</creatorcontrib><title>Interactions in the hydrogen belts of membranes: Cholesterol leaving phosphatidylcholine bilayers</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Cholesterol transfer from sonicated liposomes of phosphatidylcholine containing 10 or 30 mole percent cholesterol was measured with erythrocytes as acceptor. The activation energies of the (rate-limiting) bilayer-cholesterol dissociation were determined. In parallel experiments, phosphatidylcholine was replaced by an analog lacking the carbonyl oxygens, dietherphosphatidylcholine. The activation energies for dissociation of cholesterol from this phospholipid were three Cal/mole smaller than those for cholesterol-phosphatidylcholine dissociation, at both concentrations of cholesterol. These results demonstrate the involvement of the carbonyl oxygen in cholesterol-phospholipid bonding and support the hypothesis of lipid-lipid hydrogen bonding in the hydrogen belts of membranes.</description><subject>Animals</subject><subject>cholesterol</subject><subject>Cholesterol - metabolism</subject><subject>erythrocytes</subject><subject>Erythrocytes - metabolism</subject><subject>Hydrogen Bonding</subject><subject>Lipid Bilayers - metabolism</subject><subject>liposomes</subject><subject>Liposomes - metabolism</subject><subject>membrane structure</subject><subject>Phosphatidylcholines - metabolism</subject><subject>Rats</subject><subject>Thermodynamics</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUctKxDAUDaKM4-MPFLISXVRv2qRNXQgy-IIBNwruQpre2kjajElHmL-34wwudXUX53EP5xBywuCSAcuvACBP0pK9nUt-UTIuikTukCmDEpKUAd8l01_KPjmI8QOAMZ6XEzLJC-AMxJTop37AoM1gfR-p7enQIm1XdfDv2NMK3RCpb2iHXRV0j_GazlrvMI4i76hD_WX7d7pofVy0erD1ypkRtz3Syjq9whCPyF6jXcTj7T0kr_d3L7PHZP788DS7nScmk3JIyqaRhUmbsgKtC5RFY7RhuailaUCkIpes0EJWmclEKXleyDrlmFYasTbClNkhOdv4LoL_XI4JVWejQefG2H4ZlWQAaSayf4ksk6lgWT4S-YZogo8xYKMWwXY6rBQDtZ5ArftV636V5OpnAiVH2enWf1l1WP-Ktp2P-M0Gx7GNL4tBRWOxN1jbgGZQtbd_P_gG3jeX4Q</recordid><startdate>19840214</startdate><enddate>19840214</enddate><creator>Ramsammy, L.S.</creator><creator>Chauhan, V.P.S.</creator><creator>Box, L.L.</creator><creator>Brockerhoff, H.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19840214</creationdate><title>Interactions in the hydrogen belts of membranes: Cholesterol leaving phosphatidylcholine bilayers</title><author>Ramsammy, L.S. ; Chauhan, V.P.S. ; Box, L.L. ; Brockerhoff, H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-9ff87c2f9b0aa7e87fcac165d8cf05256817a58b3c35984678d24e2baeedc5c93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Animals</topic><topic>cholesterol</topic><topic>Cholesterol - metabolism</topic><topic>erythrocytes</topic><topic>Erythrocytes - metabolism</topic><topic>Hydrogen Bonding</topic><topic>Lipid Bilayers - metabolism</topic><topic>liposomes</topic><topic>Liposomes - metabolism</topic><topic>membrane structure</topic><topic>Phosphatidylcholines - metabolism</topic><topic>Rats</topic><topic>Thermodynamics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ramsammy, L.S.</creatorcontrib><creatorcontrib>Chauhan, V.P.S.</creatorcontrib><creatorcontrib>Box, L.L.</creatorcontrib><creatorcontrib>Brockerhoff, H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ramsammy, L.S.</au><au>Chauhan, V.P.S.</au><au>Box, L.L.</au><au>Brockerhoff, H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Interactions in the hydrogen belts of membranes: Cholesterol leaving phosphatidylcholine bilayers</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1984-02-14</date><risdate>1984</risdate><volume>118</volume><issue>3</issue><spage>743</spage><epage>746</epage><pages>743-746</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Cholesterol transfer from sonicated liposomes of phosphatidylcholine containing 10 or 30 mole percent cholesterol was measured with erythrocytes as acceptor. The activation energies of the (rate-limiting) bilayer-cholesterol dissociation were determined. In parallel experiments, phosphatidylcholine was replaced by an analog lacking the carbonyl oxygens, dietherphosphatidylcholine. The activation energies for dissociation of cholesterol from this phospholipid were three Cal/mole smaller than those for cholesterol-phosphatidylcholine dissociation, at both concentrations of cholesterol. These results demonstrate the involvement of the carbonyl oxygen in cholesterol-phospholipid bonding and support the hypothesis of lipid-lipid hydrogen bonding in the hydrogen belts of membranes.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>6704105</pmid><doi>10.1016/0006-291X(84)91457-8</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 1984-02, Vol.118 (3), p.743-746 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81002353 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals cholesterol Cholesterol - metabolism erythrocytes Erythrocytes - metabolism Hydrogen Bonding Lipid Bilayers - metabolism liposomes Liposomes - metabolism membrane structure Phosphatidylcholines - metabolism Rats Thermodynamics |
| title | Interactions in the hydrogen belts of membranes: Cholesterol leaving phosphatidylcholine bilayers |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T05%3A34%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Interactions%20in%20the%20hydrogen%20belts%20of%20membranes:%20Cholesterol%20leaving%20phosphatidylcholine%20bilayers&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Ramsammy,%20L.S.&rft.date=1984-02-14&rft.volume=118&rft.issue=3&rft.spage=743&rft.epage=746&rft.pages=743-746&rft.issn=0006-291X&rft.eissn=1090-2104&rft_id=info:doi/10.1016/0006-291X(84)91457-8&rft_dat=%3Cproquest_cross%3E13825136%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=13825136&rft_id=info:pmid/6704105&rft_els_id=0006291X84914578&rfr_iscdi=true |