Molecular cloning of the complete genome of reovirus serotype 3
All 10 genes of the Dearing strain of reovirus serotype 3 have been cloned in their entirety into pBR322. This has been proved by sequencing the terminal regions (50–100 residues long) of all 10 cloned genes; all such regions were found to be identical to the corresponding terminal regions of the do...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1984-01, Vol.133 (1), p.191-196 |
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| container_end_page | 196 |
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| container_start_page | 191 |
| container_title | Virology (New York, N.Y.) |
| container_volume | 133 |
| creator | Cashdollar, L.William Chmelo, Richard Esparza, José Hudson, Geoffrey R. Joklik, Wolfgang K. |
| description | All 10 genes of the Dearing strain of reovirus serotype 3 have been cloned in their entirety into pBR322. This has been proved by sequencing the terminal regions (50–100 residues long) of all 10 cloned genes; all such regions were found to be identical to the corresponding terminal regions of the double-stranded RNA genes that are present in reovirus particles. The lengths of the cloned reovirus genes were estimated by comparing their electrophoretic mobilities (and those of their
PstI cleavage fragments) with those of accurately known marker DNA molecules. The genes range in size from about 3825 bp for the L genes to about 1200 pb for the S4 gene. |
| doi_str_mv | 10.1016/0042-6822(84)90438-0 |
| format | Article |
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PstI cleavage fragments) with those of accurately known marker DNA molecules. The genes range in size from about 3825 bp for the L genes to about 1200 pb for the S4 gene.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/0042-6822(84)90438-0</identifier><identifier>PMID: 6546631</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Base Sequence ; Cloning, Molecular ; DNA - metabolism ; Genes, Viral ; Mammalian orthoreovirus 3 - genetics ; Molecular Weight ; Plasmids ; Reoviridae - genetics ; reovirus 3 ; RNA, Double-Stranded - genetics ; RNA, Double-Stranded - isolation & purification ; Transcription, Genetic ; transformation</subject><ispartof>Virology (New York, N.Y.), 1984-01, Vol.133 (1), p.191-196</ispartof><rights>1984</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-66fb6f3701dc01f15703f13d7b9f2377181a679d968af4665f271ebbbf84bd783</citedby><cites>FETCH-LOGICAL-c388t-66fb6f3701dc01f15703f13d7b9f2377181a679d968af4665f271ebbbf84bd783</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0042-6822(84)90438-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6546631$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cashdollar, L.William</creatorcontrib><creatorcontrib>Chmelo, Richard</creatorcontrib><creatorcontrib>Esparza, José</creatorcontrib><creatorcontrib>Hudson, Geoffrey R.</creatorcontrib><creatorcontrib>Joklik, Wolfgang K.</creatorcontrib><title>Molecular cloning of the complete genome of reovirus serotype 3</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>All 10 genes of the Dearing strain of reovirus serotype 3 have been cloned in their entirety into pBR322. This has been proved by sequencing the terminal regions (50–100 residues long) of all 10 cloned genes; all such regions were found to be identical to the corresponding terminal regions of the double-stranded RNA genes that are present in reovirus particles. The lengths of the cloned reovirus genes were estimated by comparing their electrophoretic mobilities (and those of their
PstI cleavage fragments) with those of accurately known marker DNA molecules. The genes range in size from about 3825 bp for the L genes to about 1200 pb for the S4 gene.</description><subject>Base Sequence</subject><subject>Cloning, Molecular</subject><subject>DNA - metabolism</subject><subject>Genes, Viral</subject><subject>Mammalian orthoreovirus 3 - genetics</subject><subject>Molecular Weight</subject><subject>Plasmids</subject><subject>Reoviridae - genetics</subject><subject>reovirus 3</subject><subject>RNA, Double-Stranded - genetics</subject><subject>RNA, Double-Stranded - isolation & purification</subject><subject>Transcription, Genetic</subject><subject>transformation</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1PwzAMhiMEGmPwD0DqCcGhEDdtkl5AaOJLGuIC56hNnRHUNiNpJ-3f07KJI_hi2X792noIOQV6BRT4NaVpEnOZJBcyvcxpymRM98gUaM5jylLYJ9NfySE5CuGTDrUQdEImPEs5ZzAlty-uRt3XhY907VrbLiNnou4DI-2aVY0dRktsXYNj26NbW9-HKKB33WaFETsmB6aoA57s8oy8P9y_zZ_ixevj8_xuEWsmZRdzbkpumKBQaQoGMkGZAVaJMjcJEwIkFFzkVc5lYYbXMpMIwLIsjUzLSkg2I-db35V3Xz2GTjU2aKzrokXXByVpPsb_QmAyBw7ZIEy3Qu1dCB6NWnnbFH6jgKoRsBrpqZGekqn6AazosHa28-_LBqvfpR3RYX6zneNAY23Rq6Atthor61F3qnL27wPfJFKIrA</recordid><startdate>19840101</startdate><enddate>19840101</enddate><creator>Cashdollar, L.William</creator><creator>Chmelo, Richard</creator><creator>Esparza, José</creator><creator>Hudson, Geoffrey R.</creator><creator>Joklik, Wolfgang K.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19840101</creationdate><title>Molecular cloning of the complete genome of reovirus serotype 3</title><author>Cashdollar, L.William ; Chmelo, Richard ; Esparza, José ; Hudson, Geoffrey R. ; Joklik, Wolfgang K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-66fb6f3701dc01f15703f13d7b9f2377181a679d968af4665f271ebbbf84bd783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Base Sequence</topic><topic>Cloning, Molecular</topic><topic>DNA - metabolism</topic><topic>Genes, Viral</topic><topic>Mammalian orthoreovirus 3 - genetics</topic><topic>Molecular Weight</topic><topic>Plasmids</topic><topic>Reoviridae - genetics</topic><topic>reovirus 3</topic><topic>RNA, Double-Stranded - genetics</topic><topic>RNA, Double-Stranded - isolation & purification</topic><topic>Transcription, Genetic</topic><topic>transformation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cashdollar, L.William</creatorcontrib><creatorcontrib>Chmelo, Richard</creatorcontrib><creatorcontrib>Esparza, José</creatorcontrib><creatorcontrib>Hudson, Geoffrey R.</creatorcontrib><creatorcontrib>Joklik, Wolfgang K.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cashdollar, L.William</au><au>Chmelo, Richard</au><au>Esparza, José</au><au>Hudson, Geoffrey R.</au><au>Joklik, Wolfgang K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning of the complete genome of reovirus serotype 3</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1984-01-01</date><risdate>1984</risdate><volume>133</volume><issue>1</issue><spage>191</spage><epage>196</epage><pages>191-196</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><abstract>All 10 genes of the Dearing strain of reovirus serotype 3 have been cloned in their entirety into pBR322. This has been proved by sequencing the terminal regions (50–100 residues long) of all 10 cloned genes; all such regions were found to be identical to the corresponding terminal regions of the double-stranded RNA genes that are present in reovirus particles. The lengths of the cloned reovirus genes were estimated by comparing their electrophoretic mobilities (and those of their
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| fulltext | fulltext |
| identifier | ISSN: 0042-6822 |
| ispartof | Virology (New York, N.Y.), 1984-01, Vol.133 (1), p.191-196 |
| issn | 0042-6822 1096-0341 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80999998 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present); EZB-FREE-00999 freely available EZB journals |
| subjects | Base Sequence Cloning, Molecular DNA - metabolism Genes, Viral Mammalian orthoreovirus 3 - genetics Molecular Weight Plasmids Reoviridae - genetics reovirus 3 RNA, Double-Stranded - genetics RNA, Double-Stranded - isolation & purification Transcription, Genetic transformation |
| title | Molecular cloning of the complete genome of reovirus serotype 3 |
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