Cationic metals promote sequence-directed DNA bending
A DNA segment of approximately 200 base pairs (bp) from Crithidia fasciculata kinetoplast minicircles was previously shown by electron microscopy (EM) to bend into a small circle due to its unique nucleotide sequence containing repeated blocks of 4-6 A's. When this segment was flanked by 207 bp...
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| Veröffentlicht in: | Biochemistry (Easton) 1987-06, Vol.26 (13), p.3759-3762 |
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| container_title | Biochemistry (Easton) |
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| creator | Laundon, Caroline H. Griffith, Jack D. |
| description | A DNA segment of approximately 200 base pairs (bp) from Crithidia fasciculata kinetoplast minicircles was previously shown by electron microscopy (EM) to bend into a small circle due to its unique nucleotide sequence containing repeated blocks of 4-6 A's. When this segment was flanked by 207 bp of plasmid DNA on one side and 460 bp on the other, the resulting 890-bp DNA was found to appear either relatively straight or extremely bent as visualized by EM. The bend was located one-third the distance from one end. The fraction of molecules with the most extreme bend increased from approximately 2% to 50-60% following incubation of the DNA with increasing concentrations of Zn2+, Co2+, Ba2+, and Mn2+. These observations suggest that sequence-directed bending in DNA is an inducible and not a static phenomenon. Possible roles of transitions between the bent and straight conformations in the control of gene expression are discussed. |
| doi_str_mv | 10.1021/bi00387a003 |
| format | Article |
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When this segment was flanked by 207 bp of plasmid DNA on one side and 460 bp on the other, the resulting 890-bp DNA was found to appear either relatively straight or extremely bent as visualized by EM. The bend was located one-third the distance from one end. The fraction of molecules with the most extreme bend increased from approximately 2% to 50-60% following incubation of the DNA with increasing concentrations of Zn2+, Co2+, Ba2+, and Mn2+. These observations suggest that sequence-directed bending in DNA is an inducible and not a static phenomenon. Possible roles of transitions between the bent and straight conformations in the control of gene expression are discussed.</description><identifier>ISSN: 0006-2960</identifier><identifier>EISSN: 1520-4995</identifier><identifier>DOI: 10.1021/bi00387a003</identifier><identifier>PMID: 3651411</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Animals ; Barium - pharmacology ; Base Sequence ; Biological and medical sciences ; Cations - pharmacology ; Cobalt - pharmacology ; Crithidia - genetics ; Crithidia fasciculata ; DNA - drug effects ; DNA - ultrastructure ; Fundamental and applied biological sciences. Psychology ; Magnesium - pharmacology ; Microscopy, Electron ; Molecular biophysics ; Nucleic Acid Conformation ; Protein Conformation ; Structure in molecular biology ; Tridimensional structure ; Zinc - pharmacology</subject><ispartof>Biochemistry (Easton), 1987-06, Vol.26 (13), p.3759-3762</ispartof><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a414t-e10483cf26309a5d2d0cf55a61d0fe089da4341f19dcdf7c7e6fc5ae11de1c283</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/bi00387a003$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/bi00387a003$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,778,782,2754,27065,27913,27914,56727,56777</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7586405$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3651411$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Laundon, Caroline H.</creatorcontrib><creatorcontrib>Griffith, Jack D.</creatorcontrib><title>Cationic metals promote sequence-directed DNA bending</title><title>Biochemistry (Easton)</title><addtitle>Biochemistry</addtitle><description>A DNA segment of approximately 200 base pairs (bp) from Crithidia fasciculata kinetoplast minicircles was previously shown by electron microscopy (EM) to bend into a small circle due to its unique nucleotide sequence containing repeated blocks of 4-6 A's. When this segment was flanked by 207 bp of plasmid DNA on one side and 460 bp on the other, the resulting 890-bp DNA was found to appear either relatively straight or extremely bent as visualized by EM. The bend was located one-third the distance from one end. The fraction of molecules with the most extreme bend increased from approximately 2% to 50-60% following incubation of the DNA with increasing concentrations of Zn2+, Co2+, Ba2+, and Mn2+. These observations suggest that sequence-directed bending in DNA is an inducible and not a static phenomenon. Possible roles of transitions between the bent and straight conformations in the control of gene expression are discussed.</description><subject>Animals</subject><subject>Barium - pharmacology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cations - pharmacology</subject><subject>Cobalt - pharmacology</subject><subject>Crithidia - genetics</subject><subject>Crithidia fasciculata</subject><subject>DNA - drug effects</subject><subject>DNA - ultrastructure</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Magnesium - pharmacology</subject><subject>Microscopy, Electron</subject><subject>Molecular biophysics</subject><subject>Nucleic Acid Conformation</subject><subject>Protein Conformation</subject><subject>Structure in molecular biology</subject><subject>Tridimensional structure</subject><subject>Zinc - pharmacology</subject><issn>0006-2960</issn><issn>1520-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0M9rFDEUB_AgSl2rJ8_CHEQPMvW9mSSTHMv6q7C0Quull5BNXiTrzsyazED975uyy-Kh0Ese4fvh8fgy9hbhDKHBz-sI0KrOlvcZW6BooOZai-dsAQCybrSEl-xVzpvy5dDxE3bSSoEcccHE0k5xHKKreprsNle7NPbjRFWmvzMNjmofE7mJfPXl8rxa0-Dj8Ps1exEKpjeHecp-fft6s_xRr66-XyzPV7XlyKeaELhqXWhkC9oK33hwQQgr0UMgUNpb3nIMqL3zoXMdyeCEJURP6BrVnrIP-73lqnJOnkwfs6Pt1g40ztko0FpxyZ-EKEAKpR_gpz10acw5UTC7FHub_hkE89Cm-a_Not8d1s7rnvzRHuor-ftDbrOz25Ds4GI-sk4oyUEUVu9ZzBPdHWOb_hjZtZ0wNz-vzbVs8XbVKHNb_Me9ty6bzTinoZT86IH3SYGWRg</recordid><startdate>19870630</startdate><enddate>19870630</enddate><creator>Laundon, Caroline H.</creator><creator>Griffith, Jack D.</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19870630</creationdate><title>Cationic metals promote sequence-directed DNA bending</title><author>Laundon, Caroline H. ; Griffith, Jack D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a414t-e10483cf26309a5d2d0cf55a61d0fe089da4341f19dcdf7c7e6fc5ae11de1c283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animals</topic><topic>Barium - pharmacology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cations - pharmacology</topic><topic>Cobalt - pharmacology</topic><topic>Crithidia - genetics</topic><topic>Crithidia fasciculata</topic><topic>DNA - drug effects</topic><topic>DNA - ultrastructure</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Magnesium - pharmacology</topic><topic>Microscopy, Electron</topic><topic>Molecular biophysics</topic><topic>Nucleic Acid Conformation</topic><topic>Protein Conformation</topic><topic>Structure in molecular biology</topic><topic>Tridimensional structure</topic><topic>Zinc - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Laundon, Caroline H.</creatorcontrib><creatorcontrib>Griffith, Jack D.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemistry (Easton)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Laundon, Caroline H.</au><au>Griffith, Jack D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cationic metals promote sequence-directed DNA bending</atitle><jtitle>Biochemistry (Easton)</jtitle><addtitle>Biochemistry</addtitle><date>1987-06-30</date><risdate>1987</risdate><volume>26</volume><issue>13</issue><spage>3759</spage><epage>3762</epage><pages>3759-3762</pages><issn>0006-2960</issn><eissn>1520-4995</eissn><abstract>A DNA segment of approximately 200 base pairs (bp) from Crithidia fasciculata kinetoplast minicircles was previously shown by electron microscopy (EM) to bend into a small circle due to its unique nucleotide sequence containing repeated blocks of 4-6 A's. When this segment was flanked by 207 bp of plasmid DNA on one side and 460 bp on the other, the resulting 890-bp DNA was found to appear either relatively straight or extremely bent as visualized by EM. The bend was located one-third the distance from one end. The fraction of molecules with the most extreme bend increased from approximately 2% to 50-60% following incubation of the DNA with increasing concentrations of Zn2+, Co2+, Ba2+, and Mn2+. These observations suggest that sequence-directed bending in DNA is an inducible and not a static phenomenon. Possible roles of transitions between the bent and straight conformations in the control of gene expression are discussed.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>3651411</pmid><doi>10.1021/bi00387a003</doi><tpages>4</tpages></addata></record> |
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| ispartof | Biochemistry (Easton), 1987-06, Vol.26 (13), p.3759-3762 |
| issn | 0006-2960 1520-4995 |
| language | eng |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | Animals Barium - pharmacology Base Sequence Biological and medical sciences Cations - pharmacology Cobalt - pharmacology Crithidia - genetics Crithidia fasciculata DNA - drug effects DNA - ultrastructure Fundamental and applied biological sciences. Psychology Magnesium - pharmacology Microscopy, Electron Molecular biophysics Nucleic Acid Conformation Protein Conformation Structure in molecular biology Tridimensional structure Zinc - pharmacology |
| title | Cationic metals promote sequence-directed DNA bending |
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