Primary structure of human pancreatic protease E determined by sequence analysis of the cloned mRNA

Although protease E was isolated from human pancreas over 10 years ago [Mallory, P. A., & Travis, J. (1975) Biochemistry 14, 722-729], its amino acid sequence and relationship to the elastases have not been established. We report the isolation of a cDNA clone for human pancreatic protease E and...

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Veröffentlicht in:	Biochemistry (Easton) 1987-06, Vol.26 (12), p.3447-3452
Hauptverfasser:	Shen, Wei Fang, Fletcher, Thomas S, Largman, Corey
Format:	Artikel
Sprache:	eng
Schlagworte:	550201 - Biochemistry- Tracer Techniques AMINO ACID SEQUENCE Analytical, structural and metabolic biochemistry Animals Base Sequence BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES BETA-MINUS DECAY RADIOISOTOPES Biological and medical sciences BODY cDNA Chymotrypsin - genetics Cloning, Molecular DAYS LIVING RADIOISOTOPES DIGESTIVE SYSTEM DNA DNA - analysis DNA SEQUENCING ENDOCRINE GLANDS ENZYMES Enzymes and enzyme inhibitors EVEN-ODD NUCLEI Fundamental and applied biological sciences. Psychology genes GLANDS Humans HYBRIDIZATION HYDROLASES ISOTOPES LABELLED COMPOUNDS LIGHT NUCLEI MESSENGER-RNA MOLECULAR STRUCTURE NUCLEI Nucleic Acid Hybridization NUCLEIC ACIDS nucleotide sequence ODD-ODD NUCLEI ORGANIC COMPOUNDS ORGANS PANCREAS Pancreas - enzymology Pancreatic Elastase - genetics PEPTIDE HYDROLASES PHOSPHORUS 32 PHOSPHORUS ISOTOPES proteinase E RADIOISOTOPES RECOMBINANT DNA RNA RNA, Messenger - genetics Sequence Homology, Nucleic Acid Serine Endopeptidases - genetics SERINE PROTEINASES STRUCTURAL CHEMICAL ANALYSIS SULFUR 35 SULFUR ISOTOPES
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