Specific leukotriene formation by purified human eosinophils and neutrophils
Human granulocytes isolated from peripheral blood have been described to synthesize both LTB 4 and LTC 4 from arachidonic acid. We have observed that the amount of LTC 4 produced by human granulocyte preparations is strongly dependent on the relative amount of eosinophils. To investigate a possibly...
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| Veröffentlicht in: | FEBS letters 1984-03, Vol.168 (1), p.23-28 |
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| creator | Verhagen, Jan Bruynzeel, Pieter L.B. Koedam, Johannes A. Aryan Wassink, G. de Boer, Martin Terpstra, Gerben K. Kreukniet, Johannes Veldink, Gerrit A. Vliegenthart, Johannes F.G. |
| description | Human granulocytes isolated from peripheral blood have been described to synthesize both LTB
4 and LTC
4 from arachidonic acid. We have observed that the amount of LTC
4 produced by human granulocyte preparations is strongly dependent on the relative amount of eosinophils. To investigate a possibly significant difference in leukotriene synthesis of the eosinophilic and neutrophilic granulocytes, we developed a purification method to isolate both cell types from granulocytes obtained from the blood of healthy donors. Leukotrienes were generated by incubation of the purified cells with arachidonic acid, calcium ionophore A23187, calcium-chloride and reduced glutathione. Surprisingly, eosinophils were found to produce almost exclusively the spasmogenic LTC
4. In contrast, neutrophils produce almost exclusively the chemotactic LTB
4, its ω-hydroxylated metabolite 20-hydroxy-LTB
4 and two non-enzymically formed LTB
4 isomers. |
| doi_str_mv | 10.1016/0014-5793(84)80199-4 |
| format | Article |
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4 and LTC
4 from arachidonic acid. We have observed that the amount of LTC
4 produced by human granulocyte preparations is strongly dependent on the relative amount of eosinophils. To investigate a possibly significant difference in leukotriene synthesis of the eosinophilic and neutrophilic granulocytes, we developed a purification method to isolate both cell types from granulocytes obtained from the blood of healthy donors. Leukotrienes were generated by incubation of the purified cells with arachidonic acid, calcium ionophore A23187, calcium-chloride and reduced glutathione. Surprisingly, eosinophils were found to produce almost exclusively the spasmogenic LTC
4. In contrast, neutrophils produce almost exclusively the chemotactic LTB
4, its ω-hydroxylated metabolite 20-hydroxy-LTB
4 and two non-enzymically formed LTB
4 isomers.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(84)80199-4</identifier><identifier>PMID: 6323218</identifier><identifier>CODEN: FEBLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analytical, structural and metabolic biochemistry ; Arachidonic Acid ; Arachidonic Acids - blood ; Asthma ; Biological and medical sciences ; Cell Separation ; Chromatography, High Pressure Liquid ; Eosinophil ; Eosinophils - cytology ; Eosinophils - metabolism ; Fundamental and applied biological sciences. Psychology ; Granulocyte ; Humans ; Leukotriene ; Leukotriene B4 - biosynthesis ; Lipids ; Lipoxygenase ; Neutrophil ; Neutrophils - cytology ; Neutrophils - metabolism ; Other biological molecules ; SRS-A - biosynthesis</subject><ispartof>FEBS letters, 1984-03, Vol.168 (1), p.23-28</ispartof><rights>1984</rights><rights>FEBS Letters 168 (1984) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>1984 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5614-1b136a5e91ca7f30cdef40d9bd5a466e562539c9a20294d8d10eb9b69f49531a3</citedby><cites>FETCH-LOGICAL-c5614-1b136a5e91ca7f30cdef40d9bd5a466e562539c9a20294d8d10eb9b69f49531a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0014579384801994$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9687329$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6323218$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Verhagen, Jan</creatorcontrib><creatorcontrib>Bruynzeel, Pieter L.B.</creatorcontrib><creatorcontrib>Koedam, Johannes A.</creatorcontrib><creatorcontrib>Aryan Wassink, G.</creatorcontrib><creatorcontrib>de Boer, Martin</creatorcontrib><creatorcontrib>Terpstra, Gerben K.</creatorcontrib><creatorcontrib>Kreukniet, Johannes</creatorcontrib><creatorcontrib>Veldink, Gerrit A.</creatorcontrib><creatorcontrib>Vliegenthart, Johannes F.G.</creatorcontrib><title>Specific leukotriene formation by purified human eosinophils and neutrophils</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>Human granulocytes isolated from peripheral blood have been described to synthesize both LTB
4 and LTC
4 from arachidonic acid. We have observed that the amount of LTC
4 produced by human granulocyte preparations is strongly dependent on the relative amount of eosinophils. To investigate a possibly significant difference in leukotriene synthesis of the eosinophilic and neutrophilic granulocytes, we developed a purification method to isolate both cell types from granulocytes obtained from the blood of healthy donors. Leukotrienes were generated by incubation of the purified cells with arachidonic acid, calcium ionophore A23187, calcium-chloride and reduced glutathione. Surprisingly, eosinophils were found to produce almost exclusively the spasmogenic LTC
4. In contrast, neutrophils produce almost exclusively the chemotactic LTB
4, its ω-hydroxylated metabolite 20-hydroxy-LTB
4 and two non-enzymically formed LTB
4 isomers.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Arachidonic Acid</subject><subject>Arachidonic Acids - blood</subject><subject>Asthma</subject><subject>Biological and medical sciences</subject><subject>Cell Separation</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Eosinophil</subject><subject>Eosinophils - cytology</subject><subject>Eosinophils - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Granulocyte</subject><subject>Humans</subject><subject>Leukotriene</subject><subject>Leukotriene B4 - biosynthesis</subject><subject>Lipids</subject><subject>Lipoxygenase</subject><subject>Neutrophil</subject><subject>Neutrophils - cytology</subject><subject>Neutrophils - metabolism</subject><subject>Other biological molecules</subject><subject>SRS-A - biosynthesis</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1P3DAQhq2qaLt8_IMi5VBV9BBqx47XviCVFVuQVuJQOFuOPRGmSRzspGj_PQ5Z7RH1ZM28z3z4HYS-EnxJMOE_MSYsL1eSXgj2Q2AiZc4-oSURK5pTxsVntDwgX9BxjM84xYLIBVpwWtCCiCXa_unBuNqZrIHxrx-Cgw6y2odWD853WbXL-jEkAGz2NLa6y8BH1_n-yTUx053NOhiHMMen6KjWTYSz_XuCHjc3D-vbfHv_-279a5ubkqeFSEUo1yVIYvSqpthYqBm2srKlZpxDyYuSSiN1gQvJrLAEQyUrLmsmS0o0PUHf57598C8jxEG1LhpoGt2BH6MSWEpCSpZANoMm-BgD1KoPrtVhpwhWk4lqckhNDinB1LuJaio73_cfqxbsoWjvWtK_7XUdjW7qoDvj4gGTPJ2gkAnbzNira2D3X6PV5ua6mIQpL9h7dtrnam4EydR_DoKKJt3JgHUBzKCsdx9_6A2HsaFT</recordid><startdate>19840312</startdate><enddate>19840312</enddate><creator>Verhagen, Jan</creator><creator>Bruynzeel, Pieter L.B.</creator><creator>Koedam, Johannes A.</creator><creator>Aryan Wassink, G.</creator><creator>de Boer, Martin</creator><creator>Terpstra, Gerben K.</creator><creator>Kreukniet, Johannes</creator><creator>Veldink, Gerrit A.</creator><creator>Vliegenthart, Johannes F.G.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19840312</creationdate><title>Specific leukotriene formation by purified human eosinophils and neutrophils</title><author>Verhagen, Jan ; Bruynzeel, Pieter L.B. ; Koedam, Johannes A. ; Aryan Wassink, G. ; de Boer, Martin ; Terpstra, Gerben K. ; Kreukniet, Johannes ; Veldink, Gerrit A. ; Vliegenthart, Johannes F.G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5614-1b136a5e91ca7f30cdef40d9bd5a466e562539c9a20294d8d10eb9b69f49531a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Arachidonic Acid</topic><topic>Arachidonic Acids - blood</topic><topic>Asthma</topic><topic>Biological and medical sciences</topic><topic>Cell Separation</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Eosinophil</topic><topic>Eosinophils - cytology</topic><topic>Eosinophils - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Granulocyte</topic><topic>Humans</topic><topic>Leukotriene</topic><topic>Leukotriene B4 - biosynthesis</topic><topic>Lipids</topic><topic>Lipoxygenase</topic><topic>Neutrophil</topic><topic>Neutrophils - cytology</topic><topic>Neutrophils - metabolism</topic><topic>Other biological molecules</topic><topic>SRS-A - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Verhagen, Jan</creatorcontrib><creatorcontrib>Bruynzeel, Pieter L.B.</creatorcontrib><creatorcontrib>Koedam, Johannes A.</creatorcontrib><creatorcontrib>Aryan Wassink, G.</creatorcontrib><creatorcontrib>de Boer, Martin</creatorcontrib><creatorcontrib>Terpstra, Gerben K.</creatorcontrib><creatorcontrib>Kreukniet, Johannes</creatorcontrib><creatorcontrib>Veldink, Gerrit A.</creatorcontrib><creatorcontrib>Vliegenthart, Johannes F.G.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Verhagen, Jan</au><au>Bruynzeel, Pieter L.B.</au><au>Koedam, Johannes A.</au><au>Aryan Wassink, G.</au><au>de Boer, Martin</au><au>Terpstra, Gerben K.</au><au>Kreukniet, Johannes</au><au>Veldink, Gerrit A.</au><au>Vliegenthart, Johannes F.G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Specific leukotriene formation by purified human eosinophils and neutrophils</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1984-03-12</date><risdate>1984</risdate><volume>168</volume><issue>1</issue><spage>23</spage><epage>28</epage><pages>23-28</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>Human granulocytes isolated from peripheral blood have been described to synthesize both LTB
4 and LTC
4 from arachidonic acid. We have observed that the amount of LTC
4 produced by human granulocyte preparations is strongly dependent on the relative amount of eosinophils. To investigate a possibly significant difference in leukotriene synthesis of the eosinophilic and neutrophilic granulocytes, we developed a purification method to isolate both cell types from granulocytes obtained from the blood of healthy donors. Leukotrienes were generated by incubation of the purified cells with arachidonic acid, calcium ionophore A23187, calcium-chloride and reduced glutathione. Surprisingly, eosinophils were found to produce almost exclusively the spasmogenic LTC
4. In contrast, neutrophils produce almost exclusively the chemotactic LTB
4, its ω-hydroxylated metabolite 20-hydroxy-LTB
4 and two non-enzymically formed LTB
4 isomers.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>6323218</pmid><doi>10.1016/0014-5793(84)80199-4</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEBS letters, 1984-03, Vol.168 (1), p.23-28 |
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| source | MEDLINE; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Analytical, structural and metabolic biochemistry Arachidonic Acid Arachidonic Acids - blood Asthma Biological and medical sciences Cell Separation Chromatography, High Pressure Liquid Eosinophil Eosinophils - cytology Eosinophils - metabolism Fundamental and applied biological sciences. Psychology Granulocyte Humans Leukotriene Leukotriene B4 - biosynthesis Lipids Lipoxygenase Neutrophil Neutrophils - cytology Neutrophils - metabolism Other biological molecules SRS-A - biosynthesis |
| title | Specific leukotriene formation by purified human eosinophils and neutrophils |
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