HPLC Assay for Guanidine Salts Based on Pre-Column Derivatization with Acetylacetone
A specific, isocratic HPLC assay procedure for guanidine hydrochloride is developed. A key step in the procedure, pre-column derivatization with acetytacetone, is performed under conditions that permit direct injection of the reaction mixture onto an HPLC column. Specific directions for carrying out...
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| Veröffentlicht in: | Journal of chromatographic science 1984-03, Vol.22 (3), p.99-103 |
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| container_end_page | 103 |
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| container_issue | 3 |
| container_start_page | 99 |
| container_title | Journal of chromatographic science |
| container_volume | 22 |
| creator | Palaitis, Waldemar Curran, John R. |
| description | A specific, isocratic HPLC assay procedure for guanidine hydrochloride is developed. A key step in the procedure, pre-column derivatization with acetytacetone, is performed under conditions that permit direct injection of the reaction mixture onto an HPLC column. Specific directions for carrying out the derivatization are given and the effects of reaction variables are discussed. In addition, chromatographic conditions are given for the separation of the derivative formed from other components of the reaction mixture. The derivatization also is demonstrated to be applicable to guanidine carbonate, nitrate, and sulfate. |
| doi_str_mv | 10.1093/chromsci/22.3.99 |
| format | Article |
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A key step in the procedure, pre-column derivatization with acetytacetone, is performed under conditions that permit direct injection of the reaction mixture onto an HPLC column. Specific directions for carrying out the derivatization are given and the effects of reaction variables are discussed. In addition, chromatographic conditions are given for the separation of the derivative formed from other components of the reaction mixture. 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A key step in the procedure, pre-column derivatization with acetytacetone, is performed under conditions that permit direct injection of the reaction mixture onto an HPLC column. Specific directions for carrying out the derivatization are given and the effects of reaction variables are discussed. In addition, chromatographic conditions are given for the separation of the derivative formed from other components of the reaction mixture. The derivatization also is demonstrated to be applicable to guanidine carbonate, nitrate, and sulfate.</description><subject>Analytical chemistry</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Exact sciences and technology</subject><subject>Gas chromatographic methods</subject><subject>Guanidine</subject><subject>Guanidines - analysis</subject><subject>Ketones</subject><subject>Other chromatographic methods</subject><subject>Pentanones</subject><issn>0021-9665</issn><issn>1945-239X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kM1vEzEQxS1EVULpnQuSD4jbpv5Yrz3HEGjTKpRKtFLExfJ6bdWwH8XeBdK_vq6S5jAzGr3fvJEeQu8pmVMC_Mzex6FLNpwxNudzgFdoRqEUBeOweY1mhDBaQFWJN-htSr-eV6rEMTquJJFUsRm6Xd2sl3iRktliP0R8MZk-NKF3-Idpx4Q_m-QaPPT4JrpiObRT1-MvLoa_ZgyPubLyL4z3eGHduG1N7kPv3qEjb9rkTvfzBN2df71dror194vL5WJdWF7RsWDQ1LICoLSEyjfMe1mzUggvVd1wUEY5T8AAGOKJpwJKLqiQRtXEVo1i_AR92vk-xOHP5NKou5Csa1vTu2FKWhEAUgqZQbIDbRxSis7rhxg6E7eaEv0cpH4JUjOmuQbIJx_23lPdueZwsE8u6x_3uknWtD6a3oZ0wCB_ZYpnrNhhIY3u_0E28Xd24lLo1eanvt7w86tv11IL_gRSyYwZ</recordid><startdate>198403</startdate><enddate>198403</enddate><creator>Palaitis, Waldemar</creator><creator>Curran, John R.</creator><general>Oxford University Press</general><general>Preston Publications</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198403</creationdate><title>HPLC Assay for Guanidine Salts Based on Pre-Column Derivatization with Acetylacetone</title><author>Palaitis, Waldemar ; Curran, John R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-29db769911496fd2ff7b2455f78bd398a8ef09a99a0f0f159435157a8b0c6d823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Analytical chemistry</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Exact sciences and technology</topic><topic>Gas chromatographic methods</topic><topic>Guanidine</topic><topic>Guanidines - analysis</topic><topic>Ketones</topic><topic>Other chromatographic methods</topic><topic>Pentanones</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Palaitis, Waldemar</creatorcontrib><creatorcontrib>Curran, John R.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of chromatographic science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Palaitis, Waldemar</au><au>Curran, John R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>HPLC Assay for Guanidine Salts Based on Pre-Column Derivatization with Acetylacetone</atitle><jtitle>Journal of chromatographic science</jtitle><addtitle>Journal of Chromatographic Science</addtitle><date>1984-03</date><risdate>1984</risdate><volume>22</volume><issue>3</issue><spage>99</spage><epage>103</epage><pages>99-103</pages><issn>0021-9665</issn><eissn>1945-239X</eissn><coden>JCHSBZ</coden><abstract>A specific, isocratic HPLC assay procedure for guanidine hydrochloride is developed. A key step in the procedure, pre-column derivatization with acetytacetone, is performed under conditions that permit direct injection of the reaction mixture onto an HPLC column. Specific directions for carrying out the derivatization are given and the effects of reaction variables are discussed. In addition, chromatographic conditions are given for the separation of the derivative formed from other components of the reaction mixture. The derivatization also is demonstrated to be applicable to guanidine carbonate, nitrate, and sulfate.</abstract><cop>Niles, IL</cop><pub>Oxford University Press</pub><pmid>6707182</pmid><doi>10.1093/chromsci/22.3.99</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9665 |
| ispartof | Journal of chromatographic science, 1984-03, Vol.22 (3), p.99-103 |
| issn | 0021-9665 1945-239X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80990457 |
| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy |
| subjects | Analytical chemistry Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography, High Pressure Liquid - methods Exact sciences and technology Gas chromatographic methods Guanidine Guanidines - analysis Ketones Other chromatographic methods Pentanones |
| title | HPLC Assay for Guanidine Salts Based on Pre-Column Derivatization with Acetylacetone |
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