Growth hormone response of steers injected with synthetic human pancreatic growth hormone-releasing factors
Four Holstein steers (312 kg) in a 4 X 4 Latin-square design were injected iv with 0, 100, 300 and 1,000 micrograms of a 44 amino acid growth hormone-releasing factor, hpGRF-44 NH2 in Exp. 1. Blood was collected at 20-min intervals from -1 to +6 h. All doses of hpGRF-44 NH2 stimulated an increase in...
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Veröffentlicht in: | Journal of animal science 1984-02, Vol.58 (2), p.430-435 |
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description | Four Holstein steers (312 kg) in a 4 X 4 Latin-square design were injected iv with 0, 100, 300 and 1,000 micrograms of a 44 amino acid growth hormone-releasing factor, hpGRF-44 NH2 in Exp. 1. Blood was collected at 20-min intervals from -1 to +6 h. All doses of hpGRF-44 NH2 stimulated an increase in serum growth hormone (GH) concentrations, whereas prolactin and luteinizing hormone concentrations were unaffected. Steers injected with the 1,000-micrograms dose appeared to have a biphasic release of GH that was not observed at the other doses. Amplitude of the GH peak after the 1,000-micrograms dose tended to be higher (P less than .15) than the peak caused by either the 300-micrograms or 100-micrograms dose, and the latter two were similar. Area under the GH response curve increased (P less than .05) with increasing doses of hpGRF-44 NH2. Endogenous episodic secretion of GH resumed within 6 h after injection of 100 micrograms hpGRF-44 NH2, but not after either the 300-micrograms or 1,000-micrograms dose. In Exp. 2, six Holstein steers (352 kg) in a 6 X 6 Latin-square design received an iv injection of 0, 10, 25, 50 and 100 micrograms hpGRF-44 NH2 and 100 micrograms hpGRF-40 OH. Blood was collected at 20-min intervals from -1 to +4 h, with additional samples at 5, 10 and 15 min. Four of six steers responded to 10 micrograms hpGRF-44 NH2, but all of the steers responded to all other doses of hpGRF-44 NH2 and to hpGRF-40 OH. |
doi_str_mv | 10.2527/jas1984.582430x |
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M ; Krabill, L. F ; Friedman, A. R ; Olsen, R. F</creator><creatorcontrib>Moseley, W. M ; Krabill, L. F ; Friedman, A. R ; Olsen, R. F</creatorcontrib><description>Four Holstein steers (312 kg) in a 4 X 4 Latin-square design were injected iv with 0, 100, 300 and 1,000 micrograms of a 44 amino acid growth hormone-releasing factor, hpGRF-44 NH2 in Exp. 1. Blood was collected at 20-min intervals from -1 to +6 h. All doses of hpGRF-44 NH2 stimulated an increase in serum growth hormone (GH) concentrations, whereas prolactin and luteinizing hormone concentrations were unaffected. Steers injected with the 1,000-micrograms dose appeared to have a biphasic release of GH that was not observed at the other doses. Amplitude of the GH peak after the 1,000-micrograms dose tended to be higher (P less than .15) than the peak caused by either the 300-micrograms or 100-micrograms dose, and the latter two were similar. Area under the GH response curve increased (P less than .05) with increasing doses of hpGRF-44 NH2. Endogenous episodic secretion of GH resumed within 6 h after injection of 100 micrograms hpGRF-44 NH2, but not after either the 300-micrograms or 1,000-micrograms dose. In Exp. 2, six Holstein steers (352 kg) in a 6 X 6 Latin-square design received an iv injection of 0, 10, 25, 50 and 100 micrograms hpGRF-44 NH2 and 100 micrograms hpGRF-40 OH. Blood was collected at 20-min intervals from -1 to +4 h, with additional samples at 5, 10 and 15 min. Four of six steers responded to 10 micrograms hpGRF-44 NH2, but all of the steers responded to all other doses of hpGRF-44 NH2 and to hpGRF-40 OH.</description><identifier>ISSN: 0021-8812</identifier><identifier>EISSN: 1525-3163</identifier><identifier>DOI: 10.2527/jas1984.582430x</identifier><identifier>PMID: 6423603</identifier><language>eng</language><publisher>United States: Am Soc Animal Sci</publisher><subject>Animals ; Cattle - blood ; Dose-Response Relationship, Drug ; Growth Hormone - blood ; Growth Hormone-Releasing Hormone - pharmacology ; Luteinizing Hormone - blood ; Male ; Peptide Fragments - pharmacology ; Prolactin - blood ; Stimulation, Chemical</subject><ispartof>Journal of animal science, 1984-02, Vol.58 (2), p.430-435</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c345t-900390016660ab4ec82bf761be678ec722eda3541573cd59452e76a1a3cbc91a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6423603$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moseley, W. M</creatorcontrib><creatorcontrib>Krabill, L. F</creatorcontrib><creatorcontrib>Friedman, A. R</creatorcontrib><creatorcontrib>Olsen, R. F</creatorcontrib><title>Growth hormone response of steers injected with synthetic human pancreatic growth hormone-releasing factors</title><title>Journal of animal science</title><addtitle>J Anim Sci</addtitle><description>Four Holstein steers (312 kg) in a 4 X 4 Latin-square design were injected iv with 0, 100, 300 and 1,000 micrograms of a 44 amino acid growth hormone-releasing factor, hpGRF-44 NH2 in Exp. 1. Blood was collected at 20-min intervals from -1 to +6 h. All doses of hpGRF-44 NH2 stimulated an increase in serum growth hormone (GH) concentrations, whereas prolactin and luteinizing hormone concentrations were unaffected. Steers injected with the 1,000-micrograms dose appeared to have a biphasic release of GH that was not observed at the other doses. Amplitude of the GH peak after the 1,000-micrograms dose tended to be higher (P less than .15) than the peak caused by either the 300-micrograms or 100-micrograms dose, and the latter two were similar. Area under the GH response curve increased (P less than .05) with increasing doses of hpGRF-44 NH2. Endogenous episodic secretion of GH resumed within 6 h after injection of 100 micrograms hpGRF-44 NH2, but not after either the 300-micrograms or 1,000-micrograms dose. In Exp. 2, six Holstein steers (352 kg) in a 6 X 6 Latin-square design received an iv injection of 0, 10, 25, 50 and 100 micrograms hpGRF-44 NH2 and 100 micrograms hpGRF-40 OH. Blood was collected at 20-min intervals from -1 to +4 h, with additional samples at 5, 10 and 15 min. Four of six steers responded to 10 micrograms hpGRF-44 NH2, but all of the steers responded to all other doses of hpGRF-44 NH2 and to hpGRF-40 OH.</description><subject>Animals</subject><subject>Cattle - blood</subject><subject>Dose-Response Relationship, Drug</subject><subject>Growth Hormone - blood</subject><subject>Growth Hormone-Releasing Hormone - pharmacology</subject><subject>Luteinizing Hormone - blood</subject><subject>Male</subject><subject>Peptide Fragments - pharmacology</subject><subject>Prolactin - blood</subject><subject>Stimulation, Chemical</subject><issn>0021-8812</issn><issn>1525-3163</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkE1r3DAQhkVJSLdpzz216NLcvNGHJcvHEJq0sJBDkrOQteO1tra10XjZ5N9Hy5pAD2JA88w7zEPId86WQonqeuuQ16ZcKiNKyV4_kQVXQhWSa3lGFowJXhjDxWfyBXHLGBeqVhfkQpdCaiYX5N99ioepo11MQxyBJsBdHBFobClOAAlpGLfgJ1jTQ8ggvo1TB1PwtNsPbqQ7N_oE7vix-S-qSNCDwzBuaOv8FBN-Jeet6xG-zfWSPN_9frr9U6we7v_e3qwKL0s1FTVjMj-utWauKcEb0bSV5g3oyoCvhIC1k6rkqpJ-repSCai04076xte5XJKrU-4uxZc94GSHgB763o0Q92gNy8ZKozJ4fQJ9iogJWrtLYXDpzXJmj3rtrNfOevPEjzl63wyw_uBnn7n_69TvwqY7hAQWB9f3mebHLGWssDkocz9PXOuidZsU0D4_5k0q3665qeU7OYSOYg</recordid><startdate>198402</startdate><enddate>198402</enddate><creator>Moseley, W. M</creator><creator>Krabill, L. F</creator><creator>Friedman, A. R</creator><creator>Olsen, R. F</creator><general>Am Soc Animal Sci</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198402</creationdate><title>Growth hormone response of steers injected with synthetic human pancreatic growth hormone-releasing factors</title><author>Moseley, W. M ; Krabill, L. F ; Friedman, A. R ; Olsen, R. F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c345t-900390016660ab4ec82bf761be678ec722eda3541573cd59452e76a1a3cbc91a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Animals</topic><topic>Cattle - blood</topic><topic>Dose-Response Relationship, Drug</topic><topic>Growth Hormone - blood</topic><topic>Growth Hormone-Releasing Hormone - pharmacology</topic><topic>Luteinizing Hormone - blood</topic><topic>Male</topic><topic>Peptide Fragments - pharmacology</topic><topic>Prolactin - blood</topic><topic>Stimulation, Chemical</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moseley, W. M</creatorcontrib><creatorcontrib>Krabill, L. F</creatorcontrib><creatorcontrib>Friedman, A. R</creatorcontrib><creatorcontrib>Olsen, R. F</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of animal science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moseley, W. M</au><au>Krabill, L. F</au><au>Friedman, A. R</au><au>Olsen, R. F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Growth hormone response of steers injected with synthetic human pancreatic growth hormone-releasing factors</atitle><jtitle>Journal of animal science</jtitle><addtitle>J Anim Sci</addtitle><date>1984-02</date><risdate>1984</risdate><volume>58</volume><issue>2</issue><spage>430</spage><epage>435</epage><pages>430-435</pages><issn>0021-8812</issn><eissn>1525-3163</eissn><abstract>Four Holstein steers (312 kg) in a 4 X 4 Latin-square design were injected iv with 0, 100, 300 and 1,000 micrograms of a 44 amino acid growth hormone-releasing factor, hpGRF-44 NH2 in Exp. 1. Blood was collected at 20-min intervals from -1 to +6 h. All doses of hpGRF-44 NH2 stimulated an increase in serum growth hormone (GH) concentrations, whereas prolactin and luteinizing hormone concentrations were unaffected. Steers injected with the 1,000-micrograms dose appeared to have a biphasic release of GH that was not observed at the other doses. Amplitude of the GH peak after the 1,000-micrograms dose tended to be higher (P less than .15) than the peak caused by either the 300-micrograms or 100-micrograms dose, and the latter two were similar. Area under the GH response curve increased (P less than .05) with increasing doses of hpGRF-44 NH2. Endogenous episodic secretion of GH resumed within 6 h after injection of 100 micrograms hpGRF-44 NH2, but not after either the 300-micrograms or 1,000-micrograms dose. In Exp. 2, six Holstein steers (352 kg) in a 6 X 6 Latin-square design received an iv injection of 0, 10, 25, 50 and 100 micrograms hpGRF-44 NH2 and 100 micrograms hpGRF-40 OH. Blood was collected at 20-min intervals from -1 to +4 h, with additional samples at 5, 10 and 15 min. Four of six steers responded to 10 micrograms hpGRF-44 NH2, but all of the steers responded to all other doses of hpGRF-44 NH2 and to hpGRF-40 OH.</abstract><cop>United States</cop><pub>Am Soc Animal Sci</pub><pmid>6423603</pmid><doi>10.2527/jas1984.582430x</doi><tpages>6</tpages></addata></record> |
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subjects | Animals Cattle - blood Dose-Response Relationship, Drug Growth Hormone - blood Growth Hormone-Releasing Hormone - pharmacology Luteinizing Hormone - blood Male Peptide Fragments - pharmacology Prolactin - blood Stimulation, Chemical |
title | Growth hormone response of steers injected with synthetic human pancreatic growth hormone-releasing factors |
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