The evaluation of a physical method for the quantification of inactivated poliovirus particles and its relationship to D-antigenicity and potency testing in rats
The use of a density gradient procedure for the quantification of intact, inactivated poliovirus particles in vaccine preparations is described. The procedure is both sensitive and highly reproducible and the results correlate with those of potency tests in rats and with D-antigen content as measure...
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| Veröffentlicht in: | Journal of biological standardization 1984, Vol.12 (1), p.93-99 |
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| container_end_page | 99 |
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| container_issue | 1 |
| container_start_page | 93 |
| container_title | Journal of biological standardization |
| container_volume | 12 |
| creator | Doel, T.R. Osterhaus, A.D.M.E. van Wezel, A.L. van Steenis, G. |
| description | The use of a density gradient procedure for the quantification of intact, inactivated poliovirus particles in vaccine preparations is described. The procedure is both sensitive and highly reproducible and the results correlate with those of potency tests in rats and with D-antigen content as measured by ELISA. Because of the occasional ambiguity observed with D-antigen assays, it is suggested that the density gradient procedure will provide valuable additional information for the
in vitro assessment of inactivated poliovirus preparations. |
| doi_str_mv | 10.1016/S0092-1157(84)80025-6 |
| format | Article |
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in vitro assessment of inactivated poliovirus preparations.</description><subject>Animals</subject><subject>Antigens, Viral - immunology</subject><subject>Biological and medical sciences</subject><subject>Centrifugation, Density Gradient - methods</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fundamental and applied biological sciences. 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Psychology</topic><topic>Microbiology</topic><topic>poliovirus</topic><topic>Poliovirus Vaccine, Inactivated - analysis</topic><topic>Poliovirus Vaccine, Inactivated - immunology</topic><topic>Poliovirus Vaccine, Inactivated - pharmacology</topic><topic>Rats</topic><topic>Techniques used in virology</topic><topic>Virology</topic><toplevel>online_resources</toplevel><creatorcontrib>Doel, T.R.</creatorcontrib><creatorcontrib>Osterhaus, A.D.M.E.</creatorcontrib><creatorcontrib>van Wezel, A.L.</creatorcontrib><creatorcontrib>van Steenis, G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biological standardization</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Doel, T.R.</au><au>Osterhaus, A.D.M.E.</au><au>van Wezel, A.L.</au><au>van Steenis, G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The evaluation of a physical method for the quantification of inactivated poliovirus particles and its relationship to D-antigenicity and potency testing in rats</atitle><jtitle>Journal of biological standardization</jtitle><addtitle>J Biol Stand</addtitle><date>1984</date><risdate>1984</risdate><volume>12</volume><issue>1</issue><spage>93</spage><epage>99</epage><pages>93-99</pages><issn>0092-1157</issn><coden>JBSTBI</coden><abstract>The use of a density gradient procedure for the quantification of intact, inactivated poliovirus particles in vaccine preparations is described. The procedure is both sensitive and highly reproducible and the results correlate with those of potency tests in rats and with D-antigen content as measured by ELISA. Because of the occasional ambiguity observed with D-antigen assays, it is suggested that the density gradient procedure will provide valuable additional information for the
in vitro assessment of inactivated poliovirus preparations.</abstract><cop>New York, NY</cop><pub>Elsevier Science (USA)</pub><pmid>6699027</pmid><doi>10.1016/S0092-1157(84)80025-6</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0092-1157 |
| ispartof | Journal of biological standardization, 1984, Vol.12 (1), p.93-99 |
| issn | 0092-1157 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80965318 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Antigens, Viral - immunology Biological and medical sciences Centrifugation, Density Gradient - methods Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology Microbiology poliovirus Poliovirus Vaccine, Inactivated - analysis Poliovirus Vaccine, Inactivated - immunology Poliovirus Vaccine, Inactivated - pharmacology Rats Techniques used in virology Virology |
| title | The evaluation of a physical method for the quantification of inactivated poliovirus particles and its relationship to D-antigenicity and potency testing in rats |
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