Alkaline phosphatase activity and isoenzymes in experimental fractures
Serum alkaline phosphatase activity and isoenzymes were studied after experimental fractures of the rat tibia. The enzyme activity was measured as the light absorption of p-nitrophenol, produced after hydrolysis of p-nitrophenyl phosphate. Enzyme separation was performed with a disc electrophoresis...
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| Veröffentlicht in: | Clinica chimica acta 1971-02, Vol.31 (2), p.375-380 |
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| container_title | Clinica chimica acta |
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| creator | Semb, T.H. Gudmundson, C.R. Westlin, N.E. Hallander, L.B. |
| description | Serum alkaline phosphatase activity and isoenzymes were studied after experimental fractures of the rat tibia. The enzyme activity was measured as the light absorption of
p-nitrophenol, produced after hydrolysis of
p-nitrophenyl phosphate. Enzyme separation was performed with a disc electrophoresis technique using polyacrylamide gel as supporting medium.
From 4 h after the fracture serum enzyme activity rose to reach a peak value after 24 h and then decreased again to normal values after 14 days. It is suggested that the serum enzyme increase is mainly a result of the early cell proliferation in the fracture area. In the fracture callus the enzyme activity was significantly increased with a maximum value 14 days after fracture.
Three enzyme fractions were found in serum from control rats. A similar serum enzyme pattern was seen in experimental animals. Also in fracture callus and control cortical bone of the tibia three enzyme bands were seen with a broad fast migrating zone. This fast moving zone, however, was more active in fracture callus. |
| doi_str_mv | 10.1016/0009-8981(71)90406-2 |
| format | Article |
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p-nitrophenol, produced after hydrolysis of
p-nitrophenyl phosphate. Enzyme separation was performed with a disc electrophoresis technique using polyacrylamide gel as supporting medium.
From 4 h after the fracture serum enzyme activity rose to reach a peak value after 24 h and then decreased again to normal values after 14 days. It is suggested that the serum enzyme increase is mainly a result of the early cell proliferation in the fracture area. In the fracture callus the enzyme activity was significantly increased with a maximum value 14 days after fracture.
Three enzyme fractions were found in serum from control rats. A similar serum enzyme pattern was seen in experimental animals. Also in fracture callus and control cortical bone of the tibia three enzyme bands were seen with a broad fast migrating zone. This fast moving zone, however, was more active in fracture callus.</description><identifier>ISSN: 0009-8981</identifier><identifier>EISSN: 1873-3492</identifier><identifier>DOI: 10.1016/0009-8981(71)90406-2</identifier><identifier>PMID: 5573681</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Alkaline Phosphatase - analysis ; Alkaline Phosphatase - blood ; Animals ; Electrophoresis, Disc ; Female ; Intestines - enzymology ; Isoenzymes - analysis ; Liver - enzymology ; Nitrobenzenes ; Nitrophenols ; Rats ; Tibial Fractures - blood ; Tibial Fractures - enzymology ; Time Factors</subject><ispartof>Clinica chimica acta, 1971-02, Vol.31 (2), p.375-380</ispartof><rights>1971</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-17bb448475b043eb0796a58c1a1c0dd473e3a3c3f8e15669be547ddf400d4c723</citedby><cites>FETCH-LOGICAL-c357t-17bb448475b043eb0796a58c1a1c0dd473e3a3c3f8e15669be547ddf400d4c723</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0009898171904062$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5573681$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Semb, T.H.</creatorcontrib><creatorcontrib>Gudmundson, C.R.</creatorcontrib><creatorcontrib>Westlin, N.E.</creatorcontrib><creatorcontrib>Hallander, L.B.</creatorcontrib><title>Alkaline phosphatase activity and isoenzymes in experimental fractures</title><title>Clinica chimica acta</title><addtitle>Clin Chim Acta</addtitle><description>Serum alkaline phosphatase activity and isoenzymes were studied after experimental fractures of the rat tibia. The enzyme activity was measured as the light absorption of
p-nitrophenol, produced after hydrolysis of
p-nitrophenyl phosphate. Enzyme separation was performed with a disc electrophoresis technique using polyacrylamide gel as supporting medium.
From 4 h after the fracture serum enzyme activity rose to reach a peak value after 24 h and then decreased again to normal values after 14 days. It is suggested that the serum enzyme increase is mainly a result of the early cell proliferation in the fracture area. In the fracture callus the enzyme activity was significantly increased with a maximum value 14 days after fracture.
Three enzyme fractions were found in serum from control rats. A similar serum enzyme pattern was seen in experimental animals. Also in fracture callus and control cortical bone of the tibia three enzyme bands were seen with a broad fast migrating zone. This fast moving zone, however, was more active in fracture callus.</description><subject>Alkaline Phosphatase - analysis</subject><subject>Alkaline Phosphatase - blood</subject><subject>Animals</subject><subject>Electrophoresis, Disc</subject><subject>Female</subject><subject>Intestines - enzymology</subject><subject>Isoenzymes - analysis</subject><subject>Liver - enzymology</subject><subject>Nitrobenzenes</subject><subject>Nitrophenols</subject><subject>Rats</subject><subject>Tibial Fractures - blood</subject><subject>Tibial Fractures - enzymology</subject><subject>Time Factors</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1971</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9LwzAYh4Moc06_gUJPoodqsqRNchHGcCoMvOg5pMlbFu0_k3ZYP72tGzt6CuH35Jf3fRC6JPiOYJLeY4xlLKQgN5zcSsxwGs-P0JQITmPK5PwYTQ_IKToL4WO4DhSZoEmScJoKMkWrRfGpC1dB1Gzq0Gx0qwNE2rRu69o-0pWNXKih-ulLCJGrIvhuwLsSqlYXUe4HsvMQztFJrosAF_tzht5Xj2_L53j9-vSyXKxjQxPexoRnGWOC8STDjEKGuUx1IgzRxGBrGadANTU0F0CSNJUZJIxbmzOMLTN8Tmfoetfb-Pqrg9Cq0gUDRaErqLugBJYpExIPINuBxtcheMhVM0ytfa8IVqM-NbpRoxvFifrTp8b-q31_l5VgD4_2vob8YZfDsOTWgVfBOKgMWOfBtMrW7v8PfgFJ2H8y</recordid><startdate>197102</startdate><enddate>197102</enddate><creator>Semb, T.H.</creator><creator>Gudmundson, C.R.</creator><creator>Westlin, N.E.</creator><creator>Hallander, L.B.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197102</creationdate><title>Alkaline phosphatase activity and isoenzymes in experimental fractures</title><author>Semb, T.H. ; Gudmundson, C.R. ; Westlin, N.E. ; Hallander, L.B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-17bb448475b043eb0796a58c1a1c0dd473e3a3c3f8e15669be547ddf400d4c723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1971</creationdate><topic>Alkaline Phosphatase - analysis</topic><topic>Alkaline Phosphatase - blood</topic><topic>Animals</topic><topic>Electrophoresis, Disc</topic><topic>Female</topic><topic>Intestines - enzymology</topic><topic>Isoenzymes - analysis</topic><topic>Liver - enzymology</topic><topic>Nitrobenzenes</topic><topic>Nitrophenols</topic><topic>Rats</topic><topic>Tibial Fractures - blood</topic><topic>Tibial Fractures - enzymology</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Semb, T.H.</creatorcontrib><creatorcontrib>Gudmundson, C.R.</creatorcontrib><creatorcontrib>Westlin, N.E.</creatorcontrib><creatorcontrib>Hallander, L.B.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Semb, T.H.</au><au>Gudmundson, C.R.</au><au>Westlin, N.E.</au><au>Hallander, L.B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alkaline phosphatase activity and isoenzymes in experimental fractures</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>1971-02</date><risdate>1971</risdate><volume>31</volume><issue>2</issue><spage>375</spage><epage>380</epage><pages>375-380</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><abstract>Serum alkaline phosphatase activity and isoenzymes were studied after experimental fractures of the rat tibia. The enzyme activity was measured as the light absorption of
p-nitrophenol, produced after hydrolysis of
p-nitrophenyl phosphate. Enzyme separation was performed with a disc electrophoresis technique using polyacrylamide gel as supporting medium.
From 4 h after the fracture serum enzyme activity rose to reach a peak value after 24 h and then decreased again to normal values after 14 days. It is suggested that the serum enzyme increase is mainly a result of the early cell proliferation in the fracture area. In the fracture callus the enzyme activity was significantly increased with a maximum value 14 days after fracture.
Three enzyme fractions were found in serum from control rats. A similar serum enzyme pattern was seen in experimental animals. Also in fracture callus and control cortical bone of the tibia three enzyme bands were seen with a broad fast migrating zone. This fast moving zone, however, was more active in fracture callus.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>5573681</pmid><doi>10.1016/0009-8981(71)90406-2</doi><tpages>6</tpages></addata></record> |
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| ispartof | Clinica chimica acta, 1971-02, Vol.31 (2), p.375-380 |
| issn | 0009-8981 1873-3492 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Alkaline Phosphatase - analysis Alkaline Phosphatase - blood Animals Electrophoresis, Disc Female Intestines - enzymology Isoenzymes - analysis Liver - enzymology Nitrobenzenes Nitrophenols Rats Tibial Fractures - blood Tibial Fractures - enzymology Time Factors |
| title | Alkaline phosphatase activity and isoenzymes in experimental fractures |
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