Replication of Venezuelan equine encephalomyelitis virus in suspension cell cultures grown in serum-free and defined media
Biological Sciences Laboratories, U.S. Army Biological Defense Research Centre, Fort Detrick, Frederick, Maryland, 21701 Various mammalian cells propagated in serum-free and chemically defined media yielded high titres of Venezuelan equine encephalomyelitis virus. Some difference in maximum titres w...
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| Veröffentlicht in: | Journal of general virology 1971-03, Vol.10 (3), p.231-236 |
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| container_title | Journal of general virology |
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| creator | Tribble, H.R Hearn, H.J Nagle, S.C |
| description | Biological Sciences Laboratories, U.S. Army Biological Defense Research Centre, Fort Detrick, Frederick, Maryland, 21701
Various mammalian cells propagated in serum-free and chemically defined media yielded high titres of Venezuelan equine encephalomyelitis virus. Some difference in maximum titres was noted, depending upon the medium employed. Of the two serum-free media tested, lactalbumin hydrolysate medium was more effective than the chemically defined medium in supporting virus growth. The addition of serum to serum-free cultures at the time of virus inoculation had a pronounced effect characterized by a delay followed by a burst of virus replication to very high titres. Thus, the degree of replication of Venezuelan equine encephalomyelitis virus appeared to be influenced by a variety of unknown nutritional factors.
Received 29 August 1970;
accepted 30 October 1970. |
| doi_str_mv | 10.1099/0022-1317-10-3-231 |
| format | Article |
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Various mammalian cells propagated in serum-free and chemically defined media yielded high titres of Venezuelan equine encephalomyelitis virus. Some difference in maximum titres was noted, depending upon the medium employed. Of the two serum-free media tested, lactalbumin hydrolysate medium was more effective than the chemically defined medium in supporting virus growth. The addition of serum to serum-free cultures at the time of virus inoculation had a pronounced effect characterized by a delay followed by a burst of virus replication to very high titres. Thus, the degree of replication of Venezuelan equine encephalomyelitis virus appeared to be influenced by a variety of unknown nutritional factors.
Received 29 August 1970;
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Various mammalian cells propagated in serum-free and chemically defined media yielded high titres of Venezuelan equine encephalomyelitis virus. Some difference in maximum titres was noted, depending upon the medium employed. Of the two serum-free media tested, lactalbumin hydrolysate medium was more effective than the chemically defined medium in supporting virus growth. The addition of serum to serum-free cultures at the time of virus inoculation had a pronounced effect characterized by a delay followed by a burst of virus replication to very high titres. Thus, the degree of replication of Venezuelan equine encephalomyelitis virus appeared to be influenced by a variety of unknown nutritional factors.
Received 29 August 1970;
accepted 30 October 1970.</description><subject>Albumins - pharmacology</subject><subject>animal diseases</subject><subject>animal health</subject><subject>Animals</subject><subject>Cats</subject><subject>Cattle</subject><subject>Cell Count</subject><subject>Cell Line</subject><subject>Culture Media</subject><subject>Culture Techniques</subject><subject>Encephalitis Virus, Venezuelan Equine - growth & development</subject><subject>Encephalitis Virus, Venezuelan Equine - pathogenicity</subject><subject>Encephalitis Viruses - growth & development</subject><subject>HeLa Cells</subject><subject>Immune Sera - pharmacology</subject><subject>Kidney</subject><subject>L Cells (Cell Line)</subject><subject>Mice</subject><subject>Virus Replication - drug effects</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1971</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkFtLHDEUx4NYdLX9AkIxT_UpNdedmUeR3kAotNrXkM2c7KbMJGMyqeinb6a7SJ8O5H85OT-ELhj9yGjXXVPKOWGCNYRRIggX7AitmFwrwqt8jFavhlN0lvNvSpmUqjlBJ0opyShboZcfMA3emtnHgKPDvyDAS4HBBAyPxQfAECxMOzPE8RkGP_uM__hUMvYB55InCHmJWhgGbMswlwQZb1N8Cv8ckMpIXALAJvS4B1crezxC781b9MaZIcO7wzxHD58_3d9-JXffv3y7vbkjVnTtTJyiTSM3rQMrpFkbI4wBqnrWNdZAxxhvJTcCJFXCcdX3jbOtkJQDa1uq1uIcfdj3Tik-FsizHn1e_msCxJJ1SzvFW66qke-NNsWcEzg9JT-a9KwZ1QtwvfDUC8_lRegKvIbeH9rLpp71GjkQrvrVXt_57e7JJ9BbCKOvKzY-6oryv6bLvdOZqM02-awffvJaQRkTXNVL_gJyxJSK</recordid><startdate>197103</startdate><enddate>197103</enddate><creator>Tribble, H.R</creator><creator>Hearn, H.J</creator><creator>Nagle, S.C</creator><general>Soc General Microbiol</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197103</creationdate><title>Replication of Venezuelan equine encephalomyelitis virus in suspension cell cultures grown in serum-free and defined media</title><author>Tribble, H.R ; Hearn, H.J ; Nagle, S.C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-f50774b8fec34a6aa3aae05d197cae9112842a3e4053f25dd7fc83402e1880563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1971</creationdate><topic>Albumins - pharmacology</topic><topic>animal diseases</topic><topic>animal health</topic><topic>Animals</topic><topic>Cats</topic><topic>Cattle</topic><topic>Cell Count</topic><topic>Cell Line</topic><topic>Culture Media</topic><topic>Culture Techniques</topic><topic>Encephalitis Virus, Venezuelan Equine - growth & development</topic><topic>Encephalitis Virus, Venezuelan Equine - pathogenicity</topic><topic>Encephalitis Viruses - growth & development</topic><topic>HeLa Cells</topic><topic>Immune Sera - pharmacology</topic><topic>Kidney</topic><topic>L Cells (Cell Line)</topic><topic>Mice</topic><topic>Virus Replication - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tribble, H.R</creatorcontrib><creatorcontrib>Hearn, H.J</creatorcontrib><creatorcontrib>Nagle, S.C</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tribble, H.R</au><au>Hearn, H.J</au><au>Nagle, S.C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Replication of Venezuelan equine encephalomyelitis virus in suspension cell cultures grown in serum-free and defined media</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1971-03</date><risdate>1971</risdate><volume>10</volume><issue>3</issue><spage>231</spage><epage>236</epage><pages>231-236</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><abstract>Biological Sciences Laboratories, U.S. Army Biological Defense Research Centre, Fort Detrick, Frederick, Maryland, 21701
Various mammalian cells propagated in serum-free and chemically defined media yielded high titres of Venezuelan equine encephalomyelitis virus. Some difference in maximum titres was noted, depending upon the medium employed. Of the two serum-free media tested, lactalbumin hydrolysate medium was more effective than the chemically defined medium in supporting virus growth. The addition of serum to serum-free cultures at the time of virus inoculation had a pronounced effect characterized by a delay followed by a burst of virus replication to very high titres. Thus, the degree of replication of Venezuelan equine encephalomyelitis virus appeared to be influenced by a variety of unknown nutritional factors.
Received 29 August 1970;
accepted 30 October 1970.</abstract><cop>England</cop><pub>Soc General Microbiol</pub><pmid>5554101</pmid><doi>10.1099/0022-1317-10-3-231</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0022-1317 |
| ispartof | Journal of general virology, 1971-03, Vol.10 (3), p.231-236 |
| issn | 0022-1317 1465-2099 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80952825 |
| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Albumins - pharmacology animal diseases animal health Animals Cats Cattle Cell Count Cell Line Culture Media Culture Techniques Encephalitis Virus, Venezuelan Equine - growth & development Encephalitis Virus, Venezuelan Equine - pathogenicity Encephalitis Viruses - growth & development HeLa Cells Immune Sera - pharmacology Kidney L Cells (Cell Line) Mice Virus Replication - drug effects |
| title | Replication of Venezuelan equine encephalomyelitis virus in suspension cell cultures grown in serum-free and defined media |
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