Identification of 1,25-Dihydroxycholecalciferol, a Form of Vitamin D3 Metabolically Active in the Intestine

Identification of 1,25-Dihydroxycholecalciferol, a Form of Vitamin D3 Metabolically Active in the Intestine

A biologically active vitamin D 3 metabolite (“peak V metabolite”) more polar than 25-hydroxycholecalciferol has been isolated from chicken intestines in pure form as a mono(trimethylsilyl)ether derivative. The molecule has been identified as 1,25-dihydroxycholecalciferol by means of mass spectromet...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1971-04, Vol.68 (4), p.803-804
Hauptverfasser: Holick, M F, Schnoes, H K, DeLuca, H F
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Biological Sciences: Biochemistry
Chemical Phenomena
Chemistry
Chickens
Cholecalciferol - isolation & purification
Chromatography
Ethers
Intestines - analysis
Mass Spectrometry
Spectrophotometry
Tritium
Ultraviolet Rays
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container_title Proceedings of the National Academy of Sciences - PNAS
container_volume 68
creator Holick, M F
Schnoes, H K
DeLuca, H F
description A biologically active vitamin D 3 metabolite (“peak V metabolite”) more polar than 25-hydroxycholecalciferol has been isolated from chicken intestines in pure form as a mono(trimethylsilyl)ether derivative. The molecule has been identified as 1,25-dihydroxycholecalciferol by means of mass spectrometry, ultraviolet absorption spectrophotometry, and specific chemical reactions.
doi_str_mv 10.1073/pnas.68.4.803
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subjects Animals
Biological Sciences: Biochemistry
Chemical Phenomena
Chemistry
Chickens
Cholecalciferol - isolation & purification
Chromatography
Ethers
Intestines - analysis
Mass Spectrometry
Spectrophotometry
Tritium
Ultraviolet Rays
title Identification of 1,25-Dihydroxycholecalciferol, a Form of Vitamin D3 Metabolically Active in the Intestine
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