An antibody chimera technique applied to enzyme immunoassay for human alpha-1-fetoprotein with monoclonal and polyclonal antibodies

A 2-step enzyme immunoassay (EIA) for human alpha-1-fetoprotein (AFP) is proposed, which uses covalently coupled anti-AFP IgG and anti-horseradish peroxidase (HRP) IgG (antibody chimera) binding HRP as the marker enzyme immunologically. The use of polyclonal and monoclonal anti-AFP linked to anti-HR...

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Veröffentlicht in:	Journal of immunological methods 1984-01, Vol.66 (1), p.179-185
Hauptverfasser:	Porstmann, Baerbel, Avrameas, S., Ternynck, T., Porstmann, T., Micheel, B., Guesdon, J.-L.
Format:	Artikel
Sprache:	eng
Schlagworte:	alpha-1-fetoprotein alpha-Fetoproteins - analysis alpha-Fetoproteins - immunology Analytical, structural and metabolic biochemistry Animals Antibodies, Monoclonal - immunology antibody chimeras in EIA Antigen-Antibody Complex - metabolism Binding Sites, Antibody Biological and medical sciences enzyme immunoassay Epitopes Fundamental and applied biological sciences. Psychology Glycoproteins Horseradish Peroxidase - immunology Humans Immunoenzyme Techniques - standards Immunoglobulin G - metabolism Mice Mice, Inbred BALB C Proteins Rabbits Sheep Species Specificity
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