Purification of a growth inhibitor for Ehrlich ascites mammary carcinoma cells from bovine mammary gland
A growth inhibitor for Ehrlich ascites mammary carcinoma cells in vitro has been purified from bovine mammary gland. The purification procedure involving homogenization and differential centrifugation under hypotonic conditions, ammonium sulfate precipitation, ultrafiltration, gel chromatography and...
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| Veröffentlicht in: | Experimental cell research 1984-01, Vol.150 (2), p.466-476 |
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| container_title | Experimental cell research |
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| creator | Böhmer, Frank D. Lehmann, Wolfram Schmidt, H.Eberhard Langen, Peter Grosse, Richard |
| description | A growth inhibitor for Ehrlich ascites mammary carcinoma cells in vitro has been purified from bovine mammary gland. The purification procedure involving homogenization and differential centrifugation under hypotonic conditions, ammonium sulfate precipitation, ultrafiltration, gel chromatography and preparative polyacrylamide gel electrophoresis (PAGE) yielded an inhibitor showing half-maximal inhibition of cell proliferation in concentrations of 1–3 ng protein per ml. Upon
125I labelling and analysis by SDS gel electrophoresis, most purified preparations revealed a single band of 12–14 kD, likely to be representative for the inhibitory protein. The inhibitor was shown to affect resumption of proliferation of stationary cells; however, it was inactive towards cells stimulated by incubation with medium before adding the inhibitor. The inhibitor is heat-labile, does not act by exhausting essential components of culture medium, and its action is antagonized by insulin. |
| doi_str_mv | 10.1016/0014-4827(84)90591-3 |
| format | Article |
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125I labelling and analysis by SDS gel electrophoresis, most purified preparations revealed a single band of 12–14 kD, likely to be representative for the inhibitory protein. The inhibitor was shown to affect resumption of proliferation of stationary cells; however, it was inactive towards cells stimulated by incubation with medium before adding the inhibitor. The inhibitor is heat-labile, does not act by exhausting essential components of culture medium, and its action is antagonized by insulin.</description><identifier>ISSN: 0014-4827</identifier><identifier>EISSN: 1090-2422</identifier><identifier>DOI: 10.1016/0014-4827(84)90591-3</identifier><identifier>PMID: 6363109</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Blood ; Carcinoma, Ehrlich Tumor - pathology ; Cattle ; Cell Division - drug effects ; Cell Survival - drug effects ; Chromatography, Gel ; Dose-Response Relationship, Drug ; Electrophoresis, Polyacrylamide Gel ; Epidermal Growth Factor - pharmacology ; Female ; Growth Inhibitors - antagonists & inhibitors ; Growth Inhibitors - isolation & purification ; Growth Inhibitors - pharmacology ; Hot Temperature ; Insulin - pharmacology ; Mammary Glands, Animal - analysis ; Mammary Neoplasms, Experimental - pathology</subject><ispartof>Experimental cell research, 1984-01, Vol.150 (2), p.466-476</ispartof><rights>1984</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c380t-b275896ce86f9c5ad3aa3a072f0c261bffb93a831b8f0605143a0307cbbcafaa3</citedby><cites>FETCH-LOGICAL-c380t-b275896ce86f9c5ad3aa3a072f0c261bffb93a831b8f0605143a0307cbbcafaa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-4827(84)90591-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6363109$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Böhmer, Frank D.</creatorcontrib><creatorcontrib>Lehmann, Wolfram</creatorcontrib><creatorcontrib>Schmidt, H.Eberhard</creatorcontrib><creatorcontrib>Langen, Peter</creatorcontrib><creatorcontrib>Grosse, Richard</creatorcontrib><title>Purification of a growth inhibitor for Ehrlich ascites mammary carcinoma cells from bovine mammary gland</title><title>Experimental cell research</title><addtitle>Exp Cell Res</addtitle><description>A growth inhibitor for Ehrlich ascites mammary carcinoma cells in vitro has been purified from bovine mammary gland. The purification procedure involving homogenization and differential centrifugation under hypotonic conditions, ammonium sulfate precipitation, ultrafiltration, gel chromatography and preparative polyacrylamide gel electrophoresis (PAGE) yielded an inhibitor showing half-maximal inhibition of cell proliferation in concentrations of 1–3 ng protein per ml. Upon
125I labelling and analysis by SDS gel electrophoresis, most purified preparations revealed a single band of 12–14 kD, likely to be representative for the inhibitory protein. The inhibitor was shown to affect resumption of proliferation of stationary cells; however, it was inactive towards cells stimulated by incubation with medium before adding the inhibitor. The inhibitor is heat-labile, does not act by exhausting essential components of culture medium, and its action is antagonized by insulin.</description><subject>Animals</subject><subject>Blood</subject><subject>Carcinoma, Ehrlich Tumor - pathology</subject><subject>Cattle</subject><subject>Cell Division - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Chromatography, Gel</subject><subject>Dose-Response Relationship, Drug</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Epidermal Growth Factor - pharmacology</subject><subject>Female</subject><subject>Growth Inhibitors - antagonists & inhibitors</subject><subject>Growth Inhibitors - isolation & purification</subject><subject>Growth Inhibitors - pharmacology</subject><subject>Hot Temperature</subject><subject>Insulin - pharmacology</subject><subject>Mammary Glands, Animal - analysis</subject><subject>Mammary Neoplasms, Experimental - pathology</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFq3DAQhkVpSLZp36AJOoX24HRk2VrpUighbQqBBNqcxUiW1gq2lUrelLx95e6yxxzEHP5vRj8fIR8ZXDJg4gsAa6pG1utPsvmsoFWs4m_IioGCqm7q-i1ZHZAT8i7nRwCQkoljciy44AVckf5-m4IPFucQJxo9RbpJ8e_c0zD1wYQ5JurLu-7TEGxPMdswu0xHHEdML9RismGKI1LrhiFTn-JITXwOkzswmwGn7j058jhk92E_T8nD9-vfVzfV7d2Pn1ffbivLJcyVqdetVMI6KbyyLXYckSOsaw-2Fsx4bxRHyZmRHgS0rCkph7U1xqIv7Cm52N19SvHP1uVZjyEv3XBycZu1BMXLH7yAzQ60KeacnNdPKSx9NQO9CNaLPb3Y07LR_wXrZe1sf39rRtcdlvZGS36-yz1GjZsUsn74xZRsAbhQrC3A1x3gioXn4JIuSt1kXReSs7PuYni9wT85y5TB</recordid><startdate>19840101</startdate><enddate>19840101</enddate><creator>Böhmer, Frank D.</creator><creator>Lehmann, Wolfram</creator><creator>Schmidt, H.Eberhard</creator><creator>Langen, Peter</creator><creator>Grosse, Richard</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19840101</creationdate><title>Purification of a growth inhibitor for Ehrlich ascites mammary carcinoma cells from bovine mammary gland</title><author>Böhmer, Frank D. ; Lehmann, Wolfram ; Schmidt, H.Eberhard ; Langen, Peter ; Grosse, Richard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c380t-b275896ce86f9c5ad3aa3a072f0c261bffb93a831b8f0605143a0307cbbcafaa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Animals</topic><topic>Blood</topic><topic>Carcinoma, Ehrlich Tumor - pathology</topic><topic>Cattle</topic><topic>Cell Division - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Chromatography, Gel</topic><topic>Dose-Response Relationship, Drug</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Epidermal Growth Factor - pharmacology</topic><topic>Female</topic><topic>Growth Inhibitors - antagonists & inhibitors</topic><topic>Growth Inhibitors - isolation & purification</topic><topic>Growth Inhibitors - pharmacology</topic><topic>Hot Temperature</topic><topic>Insulin - pharmacology</topic><topic>Mammary Glands, Animal - analysis</topic><topic>Mammary Neoplasms, Experimental - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Böhmer, Frank D.</creatorcontrib><creatorcontrib>Lehmann, Wolfram</creatorcontrib><creatorcontrib>Schmidt, H.Eberhard</creatorcontrib><creatorcontrib>Langen, Peter</creatorcontrib><creatorcontrib>Grosse, Richard</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Böhmer, Frank D.</au><au>Lehmann, Wolfram</au><au>Schmidt, H.Eberhard</au><au>Langen, Peter</au><au>Grosse, Richard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification of a growth inhibitor for Ehrlich ascites mammary carcinoma cells from bovine mammary gland</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>1984-01-01</date><risdate>1984</risdate><volume>150</volume><issue>2</issue><spage>466</spage><epage>476</epage><pages>466-476</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><abstract>A growth inhibitor for Ehrlich ascites mammary carcinoma cells in vitro has been purified from bovine mammary gland. The purification procedure involving homogenization and differential centrifugation under hypotonic conditions, ammonium sulfate precipitation, ultrafiltration, gel chromatography and preparative polyacrylamide gel electrophoresis (PAGE) yielded an inhibitor showing half-maximal inhibition of cell proliferation in concentrations of 1–3 ng protein per ml. Upon
125I labelling and analysis by SDS gel electrophoresis, most purified preparations revealed a single band of 12–14 kD, likely to be representative for the inhibitory protein. The inhibitor was shown to affect resumption of proliferation of stationary cells; however, it was inactive towards cells stimulated by incubation with medium before adding the inhibitor. The inhibitor is heat-labile, does not act by exhausting essential components of culture medium, and its action is antagonized by insulin.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>6363109</pmid><doi>10.1016/0014-4827(84)90591-3</doi><tpages>11</tpages></addata></record> |
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| ispartof | Experimental cell research, 1984-01, Vol.150 (2), p.466-476 |
| issn | 0014-4827 1090-2422 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Blood Carcinoma, Ehrlich Tumor - pathology Cattle Cell Division - drug effects Cell Survival - drug effects Chromatography, Gel Dose-Response Relationship, Drug Electrophoresis, Polyacrylamide Gel Epidermal Growth Factor - pharmacology Female Growth Inhibitors - antagonists & inhibitors Growth Inhibitors - isolation & purification Growth Inhibitors - pharmacology Hot Temperature Insulin - pharmacology Mammary Glands, Animal - analysis Mammary Neoplasms, Experimental - pathology |
| title | Purification of a growth inhibitor for Ehrlich ascites mammary carcinoma cells from bovine mammary gland |
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