Identification of stage-specific sporozoite antigens of Toxoplasma gondii by monoclonal antibodies
Stage-specific antigens of Toxoplasma gondii have not been reported. We developed a panel of monoclonal antibodies that identified unique surface membrane antigens on the sporozoite and oocyst wall of T. gondii. These monoclonal antibodies failed to react with T. gondii tachyzoite surface membrane a...
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| Veröffentlicht in: | The Journal of immunology (1950) 1984-01, Vol.132 (1), p.443-449 |
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| creator | Kasper, LH Bradley, MS Pfefferkorn, ER |
| description | Stage-specific antigens of Toxoplasma gondii have not been reported. We developed a panel of monoclonal antibodies that identified unique surface membrane antigens on the sporozoite and oocyst wall of T. gondii. These monoclonal antibodies failed to react with T. gondii tachyzoite surface membrane antigens in several immunologic assays. A comparison of surface membrane radioiodinated tachyzoites and sporozoites by one- and two-dimensional electrophoresis showed that T. gondii sporozoites have two major membrane proteins of approximate m.w. 67,000 and 25,000, not present in the tachyzoite stage. Two of the stage-specific monoclonal antibodies (2E4 and 5A4) were directed against the sporozoite 67,000 m.w. protein. In addition, T. gondii sporozoites are deficient in the major radioiodinated tachyzoite protein P30, which is identified by both human and mouse convalescent antitoxoplasma antisera. A convalescent antitoxoplasma serum failed to recognize sporozoite antigens by both immunoprecipitation and reaction with nitrocellulose transfer blots. |
| doi_str_mv | 10.4049/jimmunol.132.1.443 |
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We developed a panel of monoclonal antibodies that identified unique surface membrane antigens on the sporozoite and oocyst wall of T. gondii. These monoclonal antibodies failed to react with T. gondii tachyzoite surface membrane antigens in several immunologic assays. A comparison of surface membrane radioiodinated tachyzoites and sporozoites by one- and two-dimensional electrophoresis showed that T. gondii sporozoites have two major membrane proteins of approximate m.w. 67,000 and 25,000, not present in the tachyzoite stage. Two of the stage-specific monoclonal antibodies (2E4 and 5A4) were directed against the sporozoite 67,000 m.w. protein. In addition, T. gondii sporozoites are deficient in the major radioiodinated tachyzoite protein P30, which is identified by both human and mouse convalescent antitoxoplasma antisera. A convalescent antitoxoplasma serum failed to recognize sporozoite antigens by both immunoprecipitation and reaction with nitrocellulose transfer blots.</description><identifier>ISSN: 0022-1767</identifier><identifier>EISSN: 1550-6606</identifier><identifier>DOI: 10.4049/jimmunol.132.1.443</identifier><identifier>PMID: 6197455</identifier><identifier>CODEN: JOIMA3</identifier><language>eng</language><publisher>Bethesda, MD: Am Assoc Immnol</publisher><subject>Animals ; Antibodies, Monoclonal - biosynthesis ; Antibodies, Monoclonal - immunology ; Antigens, Protozoan ; Antigens, Surface - analysis ; Antigens, Surface - immunology ; Autoradiography ; Biological and medical sciences ; Epitopes ; Female ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; General aspects and techniques ; Humans ; Membrane Proteins - analysis ; Membrane Proteins - immunology ; Mice ; Mice, Inbred BALB C ; Molecular Weight ; Protozoa ; Protozoan Proteins ; Toxoplasma - growth & development ; Toxoplasma - immunology ; Toxoplasma gondii ; Toxoplasmosis, Animal - immunology ; Toxoplasmosis, Animal - parasitology</subject><ispartof>The Journal of immunology (1950), 1984-01, Vol.132 (1), p.443-449</ispartof><rights>1984 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-8b5a94794e562dd16546c555312dddc2025acebffe03f81cda874ae23e1488b63</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,4010,27904,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9593682$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6197455$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kasper, LH</creatorcontrib><creatorcontrib>Bradley, MS</creatorcontrib><creatorcontrib>Pfefferkorn, ER</creatorcontrib><title>Identification of stage-specific sporozoite antigens of Toxoplasma gondii by monoclonal antibodies</title><title>The Journal of immunology (1950)</title><addtitle>J Immunol</addtitle><description>Stage-specific antigens of Toxoplasma gondii have not been reported. We developed a panel of monoclonal antibodies that identified unique surface membrane antigens on the sporozoite and oocyst wall of T. gondii. These monoclonal antibodies failed to react with T. gondii tachyzoite surface membrane antigens in several immunologic assays. A comparison of surface membrane radioiodinated tachyzoites and sporozoites by one- and two-dimensional electrophoresis showed that T. gondii sporozoites have two major membrane proteins of approximate m.w. 67,000 and 25,000, not present in the tachyzoite stage. Two of the stage-specific monoclonal antibodies (2E4 and 5A4) were directed against the sporozoite 67,000 m.w. protein. In addition, T. gondii sporozoites are deficient in the major radioiodinated tachyzoite protein P30, which is identified by both human and mouse convalescent antitoxoplasma antisera. A convalescent antitoxoplasma serum failed to recognize sporozoite antigens by both immunoprecipitation and reaction with nitrocellulose transfer blots.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - biosynthesis</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antigens, Protozoan</subject><subject>Antigens, Surface - analysis</subject><subject>Antigens, Surface - immunology</subject><subject>Autoradiography</subject><subject>Biological and medical sciences</subject><subject>Epitopes</subject><subject>Female</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects and techniques</subject><subject>Humans</subject><subject>Membrane Proteins - analysis</subject><subject>Membrane Proteins - immunology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Molecular Weight</subject><subject>Protozoa</subject><subject>Protozoan Proteins</subject><subject>Toxoplasma - growth & development</subject><subject>Toxoplasma - immunology</subject><subject>Toxoplasma gondii</subject><subject>Toxoplasmosis, Animal - immunology</subject><subject>Toxoplasmosis, Animal - parasitology</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9rGzEQxUVpSJ2kX6BQ2EPJbV39X-lYQpMYAr0kZ6HVztoyWmm7WuOmnz5y4sbHngbN_N4bMQ-hLwQvOeb6-9YPwy6msCSMLsmSc_YBLYgQuJYSy49ogTGlNWlk8wld5LzFGEtM-Tk6l0Q3XIgFalcdxNn33tnZp1ilvsqzXUOdR3CHdpXHNKW_yc9Q2UKuIeYD9Zj-pDHYPNhqnWLnfdU-V0OKyYUUbXhl29R5yFforLchw-djvURPtz8fb-7rh193q5sfD7XjWs-1aoXVvNEchKRdR6Tg0gkhGCmvzlFMhXXQ9j1g1iviOqsaboEyIFypVrJLdP3mO07p9w7ybAafHYRgI6RdNgprhhUl_wUJaxQVuCkgfQPdlHKeoDfj5Ac7PRuCzSEB8y-BoqGGmJJAEX09uu_aAbp3yfHkZf7tOLfZ2dBPNjqf3zEtNJOKnj658evN3k9gyqlDKKbE7Pf7074XNOSf6Q</recordid><startdate>19840101</startdate><enddate>19840101</enddate><creator>Kasper, LH</creator><creator>Bradley, MS</creator><creator>Pfefferkorn, ER</creator><general>Am Assoc Immnol</general><general>American Association of Immunologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19840101</creationdate><title>Identification of stage-specific sporozoite antigens of Toxoplasma gondii by monoclonal antibodies</title><author>Kasper, LH ; Bradley, MS ; Pfefferkorn, ER</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-8b5a94794e562dd16546c555312dddc2025acebffe03f81cda874ae23e1488b63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - biosynthesis</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antigens, Protozoan</topic><topic>Antigens, Surface - analysis</topic><topic>Antigens, Surface - immunology</topic><topic>Autoradiography</topic><topic>Biological and medical sciences</topic><topic>Epitopes</topic><topic>Female</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects and techniques</topic><topic>Humans</topic><topic>Membrane Proteins - analysis</topic><topic>Membrane Proteins - immunology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Molecular Weight</topic><topic>Protozoa</topic><topic>Protozoan Proteins</topic><topic>Toxoplasma - growth & development</topic><topic>Toxoplasma - immunology</topic><topic>Toxoplasma gondii</topic><topic>Toxoplasmosis, Animal - immunology</topic><topic>Toxoplasmosis, Animal - parasitology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kasper, LH</creatorcontrib><creatorcontrib>Bradley, MS</creatorcontrib><creatorcontrib>Pfefferkorn, ER</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kasper, LH</au><au>Bradley, MS</au><au>Pfefferkorn, ER</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of stage-specific sporozoite antigens of Toxoplasma gondii by monoclonal antibodies</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>1984-01-01</date><risdate>1984</risdate><volume>132</volume><issue>1</issue><spage>443</spage><epage>449</epage><pages>443-449</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><coden>JOIMA3</coden><abstract>Stage-specific antigens of Toxoplasma gondii have not been reported. We developed a panel of monoclonal antibodies that identified unique surface membrane antigens on the sporozoite and oocyst wall of T. gondii. These monoclonal antibodies failed to react with T. gondii tachyzoite surface membrane antigens in several immunologic assays. A comparison of surface membrane radioiodinated tachyzoites and sporozoites by one- and two-dimensional electrophoresis showed that T. gondii sporozoites have two major membrane proteins of approximate m.w. 67,000 and 25,000, not present in the tachyzoite stage. Two of the stage-specific monoclonal antibodies (2E4 and 5A4) were directed against the sporozoite 67,000 m.w. protein. In addition, T. gondii sporozoites are deficient in the major radioiodinated tachyzoite protein P30, which is identified by both human and mouse convalescent antitoxoplasma antisera. A convalescent antitoxoplasma serum failed to recognize sporozoite antigens by both immunoprecipitation and reaction with nitrocellulose transfer blots.</abstract><cop>Bethesda, MD</cop><pub>Am Assoc Immnol</pub><pmid>6197455</pmid><doi>10.4049/jimmunol.132.1.443</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - immunology Antigens, Protozoan Antigens, Surface - analysis Antigens, Surface - immunology Autoradiography Biological and medical sciences Epitopes Female Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology General aspects and techniques Humans Membrane Proteins - analysis Membrane Proteins - immunology Mice Mice, Inbred BALB C Molecular Weight Protozoa Protozoan Proteins Toxoplasma - growth & development Toxoplasma - immunology Toxoplasma gondii Toxoplasmosis, Animal - immunology Toxoplasmosis, Animal - parasitology |
| title | Identification of stage-specific sporozoite antigens of Toxoplasma gondii by monoclonal antibodies |
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