In situ oxygen consumption rates of cells in V-79 multicellular spheroids during growth
The rate of consumption of oxygen by V‐79 cells in multicellular spheroids was measured as a function of the spheroid diameter. In situ consumption was equal to that of exponentially growing cells for spheroids less than 200 μm in diameter. The rate of oxygen consumption decreased for cells in spher...
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| Veröffentlicht in: | J. Cell. Physiol.; (United States) 1984-01, Vol.118 (1), p.53-61 |
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| creator | Freyer, J. P. Tustanoff, E. Franko, A. J. Sutherland, R. M. |
| description | The rate of consumption of oxygen by V‐79 cells in multicellular spheroids was measured as a function of the spheroid diameter. In situ consumption was equal to that of exponentially growing cells for spheroids less than 200 μm in diameter. The rate of oxygen consumption decreased for cells in spheroids between 200 and 400 μm diameter to a value one‐fourth the initial, then remained constant with further spheroid growth. Comparison of consumption rates for spheroid‐derived cells before and after dissociation from the spheroid structure indicated that the spheroid microenvironment accounted for only 20% of the change in oxygen consumption rate. Cell‐cell contact, cell packing, and cell volume were not critical parameters. Plateau‐phase cells had a fivefold lower rate of oxygen consumption than exponential cells, and it is postulated that the spheroid quiescent cell population accounts for a large part of the intrinsic alteration in oxygen consumption of cells in spheroids. Some other mechanism must be involved in the regulation of cellular oxygen consumption in V‐79 spheroids to account for the remainder of the reduction observed in this system. |
| doi_str_mv | 10.1002/jcp.1041180111 |
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P. ; Tustanoff, E. ; Franko, A. J. ; Sutherland, R. M.</creator><creatorcontrib>Freyer, J. P. ; Tustanoff, E. ; Franko, A. J. ; Sutherland, R. M. ; Univ. of Rochester, NY</creatorcontrib><description>The rate of consumption of oxygen by V‐79 cells in multicellular spheroids was measured as a function of the spheroid diameter. In situ consumption was equal to that of exponentially growing cells for spheroids less than 200 μm in diameter. The rate of oxygen consumption decreased for cells in spheroids between 200 and 400 μm diameter to a value one‐fourth the initial, then remained constant with further spheroid growth. Comparison of consumption rates for spheroid‐derived cells before and after dissociation from the spheroid structure indicated that the spheroid microenvironment accounted for only 20% of the change in oxygen consumption rate. Cell‐cell contact, cell packing, and cell volume were not critical parameters. Plateau‐phase cells had a fivefold lower rate of oxygen consumption than exponential cells, and it is postulated that the spheroid quiescent cell population accounts for a large part of the intrinsic alteration in oxygen consumption of cells in spheroids. Some other mechanism must be involved in the regulation of cellular oxygen consumption in V‐79 spheroids to account for the remainder of the reduction observed in this system.</description><identifier>ISSN: 0021-9541</identifier><identifier>EISSN: 1097-4652</identifier><identifier>DOI: 10.1002/jcp.1041180111</identifier><identifier>PMID: 6690452</identifier><identifier>CODEN: JCLLAX</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>ANIMAL CELLS ; Animals ; BASIC BIOLOGICAL SCIENCES ; Biological and medical sciences ; BIOLOGICAL MODELS ; Cell Count ; CELL CULTURES ; Cell Division ; Cell metabolism, cell oxidation ; Cell physiology ; Cells, Cultured ; CHO CELLS ; Cricetinae ; Cricetulus ; DISEASES ; ELEMENTS ; Fundamental and applied biological sciences. Psychology ; GROWTH ; Molecular and cellular biology ; NEOPLASMS ; NONMETALS 550500 -- Metabolism ; OXYGEN ; Oxygen Consumption ; RESPIRATION ; SPHEROIDS</subject><ispartof>J. Cell. Physiol.; (United States), 1984-01, Vol.118 (1), p.53-61</ispartof><rights>Copyright © 1984 Wiley‐Liss, Inc.</rights><rights>1984 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4151-ba8bf52611cd9dd3bb2535ea2049f15e828b77c7d0ac872c1b7346d7bd7407073</citedby><cites>FETCH-LOGICAL-c4151-ba8bf52611cd9dd3bb2535ea2049f15e828b77c7d0ac872c1b7346d7bd7407073</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcp.1041180111$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcp.1041180111$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,885,1417,4024,27923,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9673094$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6690452$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/6630763$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Freyer, J. P.</creatorcontrib><creatorcontrib>Tustanoff, E.</creatorcontrib><creatorcontrib>Franko, A. J.</creatorcontrib><creatorcontrib>Sutherland, R. M.</creatorcontrib><creatorcontrib>Univ. of Rochester, NY</creatorcontrib><title>In situ oxygen consumption rates of cells in V-79 multicellular spheroids during growth</title><title>J. Cell. Physiol.; (United States)</title><addtitle>J. Cell. Physiol</addtitle><description>The rate of consumption of oxygen by V‐79 cells in multicellular spheroids was measured as a function of the spheroid diameter. In situ consumption was equal to that of exponentially growing cells for spheroids less than 200 μm in diameter. The rate of oxygen consumption decreased for cells in spheroids between 200 and 400 μm diameter to a value one‐fourth the initial, then remained constant with further spheroid growth. Comparison of consumption rates for spheroid‐derived cells before and after dissociation from the spheroid structure indicated that the spheroid microenvironment accounted for only 20% of the change in oxygen consumption rate. Cell‐cell contact, cell packing, and cell volume were not critical parameters. Plateau‐phase cells had a fivefold lower rate of oxygen consumption than exponential cells, and it is postulated that the spheroid quiescent cell population accounts for a large part of the intrinsic alteration in oxygen consumption of cells in spheroids. Some other mechanism must be involved in the regulation of cellular oxygen consumption in V‐79 spheroids to account for the remainder of the reduction observed in this system.</description><subject>ANIMAL CELLS</subject><subject>Animals</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>Biological and medical sciences</subject><subject>BIOLOGICAL MODELS</subject><subject>Cell Count</subject><subject>CELL CULTURES</subject><subject>Cell Division</subject><subject>Cell metabolism, cell oxidation</subject><subject>Cell physiology</subject><subject>Cells, Cultured</subject><subject>CHO CELLS</subject><subject>Cricetinae</subject><subject>Cricetulus</subject><subject>DISEASES</subject><subject>ELEMENTS</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GROWTH</subject><subject>Molecular and cellular biology</subject><subject>NEOPLASMS</subject><subject>NONMETALS 550500 -- Metabolism</subject><subject>OXYGEN</subject><subject>Oxygen Consumption</subject><subject>RESPIRATION</subject><subject>SPHEROIDS</subject><issn>0021-9541</issn><issn>1097-4652</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEFv1DAQhS0EKkvhyg3JQlVvacd2EsdHtKLbllXpodCj5TjOrksSB9tRu_8er7JaxInTWDPfe-N5CH0kcEEA6OWTHtMjJ6QCQsgrtCAgeJaXBX2NFgkgmShy8ha9C-EJAIRg7ASdlKWAvKAL9Hgz4GDjhN3LbmMGrN0Qpn6M1g3Yq2gCdi3WpusCtgP-mXGB-6mLdt-aOuVxGLfGO9sE3EzeDhu88e45bt-jN63qgvlwqKfox9XXh-V1tv6-ull-WWc6JwXJalXVbUFLQnQjmobVNS1YYRSFXLSkMBWtas41b0DpilNNas7ysuF1w3PgwNkp-jz7uhCtDNpGo7fpiMHoKMuSAS9Zgs5naPTu92RClL0N-wvUYNwUZAWCCgI0gRczqL0LwZtWjt72yu8kAbmPW6a45d-4k-DTwXmqe9Mc8UO-aX52mKugVdd6NWgbjpgoOQORJ0zM2LPtzO4_S-Xt8v6fL2Sz1oZoXo5a5X_J5M4L-Xi3kitYw7erh3u5Yn8At6-mtw</recordid><startdate>198401</startdate><enddate>198401</enddate><creator>Freyer, J. P.</creator><creator>Tustanoff, E.</creator><creator>Franko, A. J.</creator><creator>Sutherland, R. M.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>198401</creationdate><title>In situ oxygen consumption rates of cells in V-79 multicellular spheroids during growth</title><author>Freyer, J. P. ; Tustanoff, E. ; Franko, A. J. ; Sutherland, R. M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4151-ba8bf52611cd9dd3bb2535ea2049f15e828b77c7d0ac872c1b7346d7bd7407073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>ANIMAL CELLS</topic><topic>Animals</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>Biological and medical sciences</topic><topic>BIOLOGICAL MODELS</topic><topic>Cell Count</topic><topic>CELL CULTURES</topic><topic>Cell Division</topic><topic>Cell metabolism, cell oxidation</topic><topic>Cell physiology</topic><topic>Cells, Cultured</topic><topic>CHO CELLS</topic><topic>Cricetinae</topic><topic>Cricetulus</topic><topic>DISEASES</topic><topic>ELEMENTS</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GROWTH</topic><topic>Molecular and cellular biology</topic><topic>NEOPLASMS</topic><topic>NONMETALS 550500 -- Metabolism</topic><topic>OXYGEN</topic><topic>Oxygen Consumption</topic><topic>RESPIRATION</topic><topic>SPHEROIDS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Freyer, J. P.</creatorcontrib><creatorcontrib>Tustanoff, E.</creatorcontrib><creatorcontrib>Franko, A. J.</creatorcontrib><creatorcontrib>Sutherland, R. M.</creatorcontrib><creatorcontrib>Univ. of Rochester, NY</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>J. Cell. Physiol.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Freyer, J. P.</au><au>Tustanoff, E.</au><au>Franko, A. J.</au><au>Sutherland, R. M.</au><aucorp>Univ. of Rochester, NY</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In situ oxygen consumption rates of cells in V-79 multicellular spheroids during growth</atitle><jtitle>J. Cell. Physiol.; (United States)</jtitle><addtitle>J. Cell. Physiol</addtitle><date>1984-01</date><risdate>1984</risdate><volume>118</volume><issue>1</issue><spage>53</spage><epage>61</epage><pages>53-61</pages><issn>0021-9541</issn><eissn>1097-4652</eissn><coden>JCLLAX</coden><abstract>The rate of consumption of oxygen by V‐79 cells in multicellular spheroids was measured as a function of the spheroid diameter. In situ consumption was equal to that of exponentially growing cells for spheroids less than 200 μm in diameter. The rate of oxygen consumption decreased for cells in spheroids between 200 and 400 μm diameter to a value one‐fourth the initial, then remained constant with further spheroid growth. Comparison of consumption rates for spheroid‐derived cells before and after dissociation from the spheroid structure indicated that the spheroid microenvironment accounted for only 20% of the change in oxygen consumption rate. Cell‐cell contact, cell packing, and cell volume were not critical parameters. Plateau‐phase cells had a fivefold lower rate of oxygen consumption than exponential cells, and it is postulated that the spheroid quiescent cell population accounts for a large part of the intrinsic alteration in oxygen consumption of cells in spheroids. Some other mechanism must be involved in the regulation of cellular oxygen consumption in V‐79 spheroids to account for the remainder of the reduction observed in this system.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>6690452</pmid><doi>10.1002/jcp.1041180111</doi><tpages>9</tpages></addata></record> |
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| ispartof | J. Cell. Physiol.; (United States), 1984-01, Vol.118 (1), p.53-61 |
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| subjects | ANIMAL CELLS Animals BASIC BIOLOGICAL SCIENCES Biological and medical sciences BIOLOGICAL MODELS Cell Count CELL CULTURES Cell Division Cell metabolism, cell oxidation Cell physiology Cells, Cultured CHO CELLS Cricetinae Cricetulus DISEASES ELEMENTS Fundamental and applied biological sciences. Psychology GROWTH Molecular and cellular biology NEOPLASMS NONMETALS 550500 -- Metabolism OXYGEN Oxygen Consumption RESPIRATION SPHEROIDS |
| title | In situ oxygen consumption rates of cells in V-79 multicellular spheroids during growth |
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