Expression of human α 1-antitrypsin in Escherichia coli

Complementary DNA coding for human α 1-antitrypsin has been placed under the control of the λP R promotor carrier by the expression vector pCQV2 [1]. In conditions which allow transcription from this promotor (thermoinactivation of the repressor), Escherichia coli cells harbouring the recombinant pl...

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Veröffentlicht in:FEBS letters 1984-01, Vol.166 (1), p.67-70
Hauptverfasser: Bollen, Alex, Loriau, Rosette, Herzog, Albert, Hérion, Pascal
Format: Artikel
Sprache:eng
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Zusammenfassung:Complementary DNA coding for human α 1-antitrypsin has been placed under the control of the λP R promotor carrier by the expression vector pCQV2 [1]. In conditions which allow transcription from this promotor (thermoinactivation of the repressor), Escherichia coli cells harbouring the recombinant plasmid pULB1114 express human α 1-antitrypsin (± 9000 molecules/cell). The product has a M r of 44 000, corresponding to mature unglycosylated α 1-antitrypsin.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(84)80046-0