Expression of human α 1-antitrypsin in Escherichia coli
Complementary DNA coding for human α 1-antitrypsin has been placed under the control of the λP R promotor carrier by the expression vector pCQV2 [1]. In conditions which allow transcription from this promotor (thermoinactivation of the repressor), Escherichia coli cells harbouring the recombinant pl...
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Veröffentlicht in: | FEBS letters 1984-01, Vol.166 (1), p.67-70 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Complementary DNA coding for human α
1-antitrypsin has been placed under the control of the λP
R promotor carrier by the expression vector pCQV2 [1]. In conditions which allow transcription from this promotor (thermoinactivation of the repressor),
Escherichia coli cells harbouring the recombinant plasmid pULB1114 express human α
1-antitrypsin (± 9000 molecules/cell). The product has a
M
r of 44 000, corresponding to mature unglycosylated α
1-antitrypsin. |
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ISSN: | 0014-5793 1873-3468 |
DOI: | 10.1016/0014-5793(84)80046-0 |