Human Mucosal Langerhans Cells: Postmortem Identification of Regional Variations in Oral Mucosa
Modified ATPase histochemistry was used to identify and count Langerhans cells (LC) in autopsy tissue from 8 oral mucosal sites, 8–20 h postmortem. The specificity of the ATPase method was confirmed on serial sections with indirect immunofluorescence using monoclonal antibodies OKT6 and antihuman Ia...
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| Veröffentlicht in: | Journal of investigative dermatology 1984-01, Vol.82 (1), p.21-24 |
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| description | Modified ATPase histochemistry was used to identify and count Langerhans cells (LC) in autopsy tissue from 8 oral mucosal sites, 8–20 h postmortem. The specificity of the ATPase method was confirmed on serial sections with indirect immunofluorescence using monoclonal antibodies OKT6 and antihuman Ia. Average LC counts on ATPase-stained epithelial sheets from each of the 8 sites ranged from 160–550 LC/mm2. Nonkeratinized mucosae of the soft palate, ventral tongue, lip, and floor of the mouth had the highest counts (mean ± SD, 508 ± 110 LC/mm2, n = 24), and keratinized mucosae of the hard palate and gingiva had the lowest counts (201 ± 97 LC/mm2 n = 8). LC frequency was variable in 2 sites: In the dorsal tongue, LC occurred on only one side of filiform papillae and were absent from regularly recurring areas of interpapillary epithelium. In the cheek mucosa, LC clustered around connective tissue papillae and their numbers showed marked individual variation (130–650 LC/mm2). The number of LC in nonkeratinized oral mucosa is approximately the same as in skin, but keratinized oral mucosa has fewer LC. The frequency of oral mucosal LC varies inversely with the degree of keratinization. There are regions of the oral mucosa that have no LC. |
| doi_str_mv | 10.1111/1523-1747.ep12259038 |
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The specificity of the ATPase method was confirmed on serial sections with indirect immunofluorescence using monoclonal antibodies OKT6 and antihuman Ia. Average LC counts on ATPase-stained epithelial sheets from each of the 8 sites ranged from 160–550 LC/mm2. Nonkeratinized mucosae of the soft palate, ventral tongue, lip, and floor of the mouth had the highest counts (mean ± SD, 508 ± 110 LC/mm2, n = 24), and keratinized mucosae of the hard palate and gingiva had the lowest counts (201 ± 97 LC/mm2 n = 8). LC frequency was variable in 2 sites: In the dorsal tongue, LC occurred on only one side of filiform papillae and were absent from regularly recurring areas of interpapillary epithelium. In the cheek mucosa, LC clustered around connective tissue papillae and their numbers showed marked individual variation (130–650 LC/mm2). The number of LC in nonkeratinized oral mucosa is approximately the same as in skin, but keratinized oral mucosa has fewer LC. The frequency of oral mucosal LC varies inversely with the degree of keratinization. There are regions of the oral mucosa that have no LC.</description><identifier>ISSN: 0022-202X</identifier><identifier>EISSN: 1523-1747</identifier><identifier>DOI: 10.1111/1523-1747.ep12259038</identifier><identifier>PMID: 6228611</identifier><identifier>CODEN: JIDEAE</identifier><language>eng</language><publisher>Danvers, MA: Elsevier Inc</publisher><subject>Adenosine Triphosphatases - analysis ; Adenosinetriphosphatase ; Aged ; Autopsy ; Biological and medical sciences ; Cell Count ; Cheek ; Cheek - cytology ; Cleft lip/palate ; Connective tissues ; Dermatology ; Epithelium ; Female ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; Gingiva ; Gingiva - cytology ; Histochemistry ; Histocytochemistry ; Humans ; Immunofluorescence ; Keratinization ; Langerhans cells ; Langerhans Cells - cytology ; Langerhans Cells - enzymology ; Male ; Middle Aged ; Monoclonal antibodies ; Mouth ; Mouth Mucosa - cytology ; Mouth. Exocrine and endocrine salivary glands. Teeth. Esophagus ; Mucosa ; Palate ; Palate - cytology ; Papillae ; Skin ; Tongue ; Tongue - cytology ; Vertebrates: digestive system</subject><ispartof>Journal of investigative dermatology, 1984-01, Vol.82 (1), p.21-24</ispartof><rights>1984 The Society for Investigative Dermatology, Inc</rights><rights>1984 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c463t-b1712459d9e65059353146f081626471e003bb8967f7bea0bfad8019930db31e3</citedby><cites>FETCH-LOGICAL-c463t-b1712459d9e65059353146f081626471e003bb8967f7bea0bfad8019930db31e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,4010,27904,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9731432$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6228611$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Daniels, Troy E.</creatorcontrib><title>Human Mucosal Langerhans Cells: Postmortem Identification of Regional Variations in Oral Mucosa</title><title>Journal of investigative dermatology</title><addtitle>J Invest Dermatol</addtitle><description>Modified ATPase histochemistry was used to identify and count Langerhans cells (LC) in autopsy tissue from 8 oral mucosal sites, 8–20 h postmortem. The specificity of the ATPase method was confirmed on serial sections with indirect immunofluorescence using monoclonal antibodies OKT6 and antihuman Ia. Average LC counts on ATPase-stained epithelial sheets from each of the 8 sites ranged from 160–550 LC/mm2. Nonkeratinized mucosae of the soft palate, ventral tongue, lip, and floor of the mouth had the highest counts (mean ± SD, 508 ± 110 LC/mm2, n = 24), and keratinized mucosae of the hard palate and gingiva had the lowest counts (201 ± 97 LC/mm2 n = 8). LC frequency was variable in 2 sites: In the dorsal tongue, LC occurred on only one side of filiform papillae and were absent from regularly recurring areas of interpapillary epithelium. In the cheek mucosa, LC clustered around connective tissue papillae and their numbers showed marked individual variation (130–650 LC/mm2). The number of LC in nonkeratinized oral mucosa is approximately the same as in skin, but keratinized oral mucosa has fewer LC. The frequency of oral mucosal LC varies inversely with the degree of keratinization. There are regions of the oral mucosa that have no LC.</description><subject>Adenosine Triphosphatases - analysis</subject><subject>Adenosinetriphosphatase</subject><subject>Aged</subject><subject>Autopsy</subject><subject>Biological and medical sciences</subject><subject>Cell Count</subject><subject>Cheek</subject><subject>Cheek - cytology</subject><subject>Cleft lip/palate</subject><subject>Connective tissues</subject><subject>Dermatology</subject><subject>Epithelium</subject><subject>Female</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gingiva</subject><subject>Gingiva - cytology</subject><subject>Histochemistry</subject><subject>Histocytochemistry</subject><subject>Humans</subject><subject>Immunofluorescence</subject><subject>Keratinization</subject><subject>Langerhans cells</subject><subject>Langerhans Cells - cytology</subject><subject>Langerhans Cells - enzymology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Monoclonal antibodies</subject><subject>Mouth</subject><subject>Mouth Mucosa - cytology</subject><subject>Mouth. Exocrine and endocrine salivary glands. Teeth. Esophagus</subject><subject>Mucosa</subject><subject>Palate</subject><subject>Palate - cytology</subject><subject>Papillae</subject><subject>Skin</subject><subject>Tongue</subject><subject>Tongue - cytology</subject><subject>Vertebrates: digestive system</subject><issn>0022-202X</issn><issn>1523-1747</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UV1rFDEUDWKp69p_oJAHKb5Mm4-ZfPgglEVtYaVF2uJbyGTu1MhMsiYzQv99s-6yfWteEs4959zccxF6T8kZLeecNoxXVNbyDDaUsUYTrl6hxQF-jRaEMFYxwn69QW9z_kMIFXWjjtGxYEwJShfIXM6jDfjH7GK2A17b8ADptw0Zr2AY8md8E_M0xjTBiK86CJPvvbOTjwHHHv-Eh_Iqunub_H80Yx_wdSrQzvIdOurtkOFkfy_R3bevt6vLan39_Wp1sa5cLfhUtVRSVje60yAa0mjecFqLnigqmKglBUJ42yotZC9bsKTtbacI1ZqTruUU-BKd7nw3Kf6dIU9m9NmVEWyAOGejiGay1qIQP71IpEw1RHFZrJeo3lFdijkn6M0m-dGmR0OJ2a7AbLM226zN8wqK7MO-w9yO0B1E-8xL_eO-brOzQ59scD4faFqW0TkrtC87GpTY_nlIJjsPwUHnE7jJdNG__I8nFW-hLQ</recordid><startdate>198401</startdate><enddate>198401</enddate><creator>Daniels, Troy E.</creator><general>Elsevier Inc</general><general>Nature Publishing</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>198401</creationdate><title>Human Mucosal Langerhans Cells: Postmortem Identification of Regional Variations in Oral Mucosa</title><author>Daniels, Troy E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c463t-b1712459d9e65059353146f081626471e003bb8967f7bea0bfad8019930db31e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Adenosine Triphosphatases - analysis</topic><topic>Adenosinetriphosphatase</topic><topic>Aged</topic><topic>Autopsy</topic><topic>Biological and medical sciences</topic><topic>Cell Count</topic><topic>Cheek</topic><topic>Cheek - cytology</topic><topic>Cleft lip/palate</topic><topic>Connective tissues</topic><topic>Dermatology</topic><topic>Epithelium</topic><topic>Female</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gingiva</topic><topic>Gingiva - cytology</topic><topic>Histochemistry</topic><topic>Histocytochemistry</topic><topic>Humans</topic><topic>Immunofluorescence</topic><topic>Keratinization</topic><topic>Langerhans cells</topic><topic>Langerhans Cells - cytology</topic><topic>Langerhans Cells - enzymology</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Monoclonal antibodies</topic><topic>Mouth</topic><topic>Mouth Mucosa - cytology</topic><topic>Mouth. Exocrine and endocrine salivary glands. Teeth. Esophagus</topic><topic>Mucosa</topic><topic>Palate</topic><topic>Palate - cytology</topic><topic>Papillae</topic><topic>Skin</topic><topic>Tongue</topic><topic>Tongue - cytology</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Daniels, Troy E.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of investigative dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Daniels, Troy E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human Mucosal Langerhans Cells: Postmortem Identification of Regional Variations in Oral Mucosa</atitle><jtitle>Journal of investigative dermatology</jtitle><addtitle>J Invest Dermatol</addtitle><date>1984-01</date><risdate>1984</risdate><volume>82</volume><issue>1</issue><spage>21</spage><epage>24</epage><pages>21-24</pages><issn>0022-202X</issn><eissn>1523-1747</eissn><coden>JIDEAE</coden><abstract>Modified ATPase histochemistry was used to identify and count Langerhans cells (LC) in autopsy tissue from 8 oral mucosal sites, 8–20 h postmortem. The specificity of the ATPase method was confirmed on serial sections with indirect immunofluorescence using monoclonal antibodies OKT6 and antihuman Ia. Average LC counts on ATPase-stained epithelial sheets from each of the 8 sites ranged from 160–550 LC/mm2. Nonkeratinized mucosae of the soft palate, ventral tongue, lip, and floor of the mouth had the highest counts (mean ± SD, 508 ± 110 LC/mm2, n = 24), and keratinized mucosae of the hard palate and gingiva had the lowest counts (201 ± 97 LC/mm2 n = 8). LC frequency was variable in 2 sites: In the dorsal tongue, LC occurred on only one side of filiform papillae and were absent from regularly recurring areas of interpapillary epithelium. In the cheek mucosa, LC clustered around connective tissue papillae and their numbers showed marked individual variation (130–650 LC/mm2). The number of LC in nonkeratinized oral mucosa is approximately the same as in skin, but keratinized oral mucosa has fewer LC. The frequency of oral mucosal LC varies inversely with the degree of keratinization. There are regions of the oral mucosa that have no LC.</abstract><cop>Danvers, MA</cop><pub>Elsevier Inc</pub><pmid>6228611</pmid><doi>10.1111/1523-1747.ep12259038</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adenosine Triphosphatases - analysis Adenosinetriphosphatase Aged Autopsy Biological and medical sciences Cell Count Cheek Cheek - cytology Cleft lip/palate Connective tissues Dermatology Epithelium Female Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Gingiva Gingiva - cytology Histochemistry Histocytochemistry Humans Immunofluorescence Keratinization Langerhans cells Langerhans Cells - cytology Langerhans Cells - enzymology Male Middle Aged Monoclonal antibodies Mouth Mouth Mucosa - cytology Mouth. Exocrine and endocrine salivary glands. Teeth. Esophagus Mucosa Palate Palate - cytology Papillae Skin Tongue Tongue - cytology Vertebrates: digestive system |
| title | Human Mucosal Langerhans Cells: Postmortem Identification of Regional Variations in Oral Mucosa |
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