A method for isolating aortic mitochondria exhibiting high respiratory control

A method for isolating tightly coupled mitochondria from an intima-media strip of swine aorta has been devised. The most important differences from previous methods were the use of a special device for mincing the aorta into a fine gruel before the homogenization, the incubation of the homogenate wi...

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Veröffentlicht in:	Biochemical medicine 1970-08, Vol.4 (1), p.47-54
Hauptverfasser:	Morrison, E.S., Scott, R.F., Kroms, M., Pastori, S.J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amobarbital - pharmacology Animals Aorta - cytology Aorta - drug effects Aorta - enzymology Aorta - metabolism Dinitrophenols - pharmacology Electron Transport Complex IV - analysis Glutamates - metabolism Hydroxybutyrates - metabolism Malates - metabolism Male Malonates - pharmacology Methods Microscopy, Electron Mitochondria - drug effects Mitochondria - enzymology Mitochondria - metabolism Oxidative Phosphorylation Oxygen Consumption Pancreatic Elastase - pharmacology Polarography Pyruvates - metabolism Succinates - metabolism Swine
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