Direct Hybridization of Sorted Human Chromosomes: Localization of the Y Chromosome on the Flow Karyotype

A method is described for directly hybridizing a small number of sorted chromosomes with specific DNA probes. The chromosomes are analyzed by flow cytometry and sorted by deflecting the droplets containing the desired chromosomes onto a nitrocellulose filter. By using probes specific for the human Y...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1983-12, Vol.80 (24), p.7571-7575
Hauptverfasser:	Bernheim, Alain, Metezeau, Philippe, Guellaën, Georges, Fellous, Marc, Goldberg, Michel E., Berger, Roland
Format:	Artikel
Sprache:	eng
Schlagworte:	Burkitt Lymphoma Cell Line Cell lines Cellulose nitrate Chromosome polymorphism Chromosomes Cloning, Molecular DNA probes DNA, Neoplasm - genetics Flow Cytometry Fluorescence Human Y chromosome Humans Karyotype Karyotyping Male Nucleic Acid Hybridization Sex chromosomes Y chromosome Y Chromosome - physiology
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