Initial kinetics of DNA degradation by chicken crude acid deoxyribonuclease
The ability of the crude acid DNase extracted from chicken erythrocytes to degrade native DNA into rod-like sub-units of 5.5 ±0.5×10 5 daltons, has previously been attributed to a narrow specificity of the enzyme (1). In attempting to confirm this result, we have been conducted to conclude that such...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1971-02, Vol.42 (4), p.627-633 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Barthélémy-Clavey, Véronique Sicard, Philippe J. Aubel-Sadron, Geneviève |
| description | The ability of the crude acid DNase extracted from chicken erythrocytes to degrade native DNA into rod-like sub-units of 5.5 ±0.5×10
5 daltons, has previously been attributed to a narrow specificity of the enzyme (1).
In attempting to confirm this result, we have been conducted to conclude that such a specificity does not exist. The limited DNA degradation arises from inner pH fluctuations of the reaction medium, originating in its complexity. An elimination of these fluctuations allows the degradation to proceed up to the nucleotide stage, so that limited DNA degradation must be considered as an artefact. |
| doi_str_mv | 10.1016/0006-291X(71)90534-1 |
| format | Article |
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5 daltons, has previously been attributed to a narrow specificity of the enzyme (1).
In attempting to confirm this result, we have been conducted to conclude that such a specificity does not exist. The limited DNA degradation arises from inner pH fluctuations of the reaction medium, originating in its complexity. An elimination of these fluctuations allows the degradation to proceed up to the nucleotide stage, so that limited DNA degradation must be considered as an artefact.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(71)90534-1</identifier><identifier>PMID: 5543944</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Chickens ; Deoxyribonucleases - metabolism ; DNA ; DNA - metabolism ; DNA, Bacterial</subject><ispartof>Biochemical and biophysical research communications, 1971-02, Vol.42 (4), p.627-633</ispartof><rights>1971</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c245t-5036335cc393dfae6e066a3b50fa81a8d444e2124700aa5f0ff17805594d3ce3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-291X(71)90534-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5543944$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Barthélémy-Clavey, Véronique</creatorcontrib><creatorcontrib>Sicard, Philippe J.</creatorcontrib><creatorcontrib>Aubel-Sadron, Geneviève</creatorcontrib><title>Initial kinetics of DNA degradation by chicken crude acid deoxyribonuclease</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The ability of the crude acid DNase extracted from chicken erythrocytes to degrade native DNA into rod-like sub-units of 5.5 ±0.5×10
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In attempting to confirm this result, we have been conducted to conclude that such a specificity does not exist. The limited DNA degradation arises from inner pH fluctuations of the reaction medium, originating in its complexity. An elimination of these fluctuations allows the degradation to proceed up to the nucleotide stage, so that limited DNA degradation must be considered as an artefact.</description><subject>Animals</subject><subject>Chickens</subject><subject>Deoxyribonucleases - metabolism</subject><subject>DNA</subject><subject>DNA - metabolism</subject><subject>DNA, Bacterial</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1971</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1P3DAURa0KBAPtPygiq6pdhL4XfyTZICFKAYHoolTqzvLYz2DIxNROEPPvm2FGLFm9xT336ukw9hnhCAHVdwBQZdXi3681fmtBclHiBzZDaKGsEMQWm70hu2wv5wcARKHaHbYjpeCtEDN2ddmHIZiueAw9DcHmIvrix81J4eguGWeGEPtivizsfbCP1Bc2jY4KY4ObiPiyTGEe-9F2ZDJ9ZNvedJk-be4-u_15dnt6UV7_Or88PbkubSXkUErginNpLW-584YUgVKGzyV406BpnBCCKqxEDWCM9OA91g1I2QrHLfF99mU9-5Tiv5HyoBchW-o601Mcs26gaabpdgLFGrQp5pzI66cUFiYtNYJeKdQrP3rlR9eoXxVqnGoHm_1xviD3Vto4m_LDde5N1OYuhaz__K4AOVRcVUrVE3G8Jmiy8Bwo6WwD9ZZcSGQH7WJ4_4X_glSIwg</recordid><startdate>19710219</startdate><enddate>19710219</enddate><creator>Barthélémy-Clavey, Véronique</creator><creator>Sicard, Philippe J.</creator><creator>Aubel-Sadron, Geneviève</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19710219</creationdate><title>Initial kinetics of DNA degradation by chicken crude acid deoxyribonuclease</title><author>Barthélémy-Clavey, Véronique ; Sicard, Philippe J. ; Aubel-Sadron, Geneviève</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c245t-5036335cc393dfae6e066a3b50fa81a8d444e2124700aa5f0ff17805594d3ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1971</creationdate><topic>Animals</topic><topic>Chickens</topic><topic>Deoxyribonucleases - metabolism</topic><topic>DNA</topic><topic>DNA - metabolism</topic><topic>DNA, Bacterial</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Barthélémy-Clavey, Véronique</creatorcontrib><creatorcontrib>Sicard, Philippe J.</creatorcontrib><creatorcontrib>Aubel-Sadron, Geneviève</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barthélémy-Clavey, Véronique</au><au>Sicard, Philippe J.</au><au>Aubel-Sadron, Geneviève</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Initial kinetics of DNA degradation by chicken crude acid deoxyribonuclease</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1971-02-19</date><risdate>1971</risdate><volume>42</volume><issue>4</issue><spage>627</spage><epage>633</epage><pages>627-633</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The ability of the crude acid DNase extracted from chicken erythrocytes to degrade native DNA into rod-like sub-units of 5.5 ±0.5×10
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In attempting to confirm this result, we have been conducted to conclude that such a specificity does not exist. The limited DNA degradation arises from inner pH fluctuations of the reaction medium, originating in its complexity. An elimination of these fluctuations allows the degradation to proceed up to the nucleotide stage, so that limited DNA degradation must be considered as an artefact.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>5543944</pmid><doi>10.1016/0006-291X(71)90534-1</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1971-02, Vol.42 (4), p.627-633 |
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| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Chickens Deoxyribonucleases - metabolism DNA DNA - metabolism DNA, Bacterial |
| title | Initial kinetics of DNA degradation by chicken crude acid deoxyribonuclease |
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