Activity of the hybrid trp-lac ( tac) promoter of Escherichia coli in Pseudomonas putida. Construction of broad-host-range, controlled-expression vectors

A broad-host-range vector, pKT240, containing the structural gene ( aph) for aminoglycoside phosphotransferase (APH), without promoter, has been constructed. Insertion of DNA fragments carrying promoters upstreamof the aph gene into the unique EcoRI site of this vector results in the expression of t...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Gene 1983-12, Vol.26 (2), p.273-282
Hauptverfasser:	Bagdasarian, Miroslawa M., Amann, Egon, Lurz, Rudolf, Rückert, Beate, Bagdasarian, Michael
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Catechol 2,3-Dioxygenase cloning vectors cloning vehicles Cloning, Molecular Dioxygenases Drug Resistance, Microbial Escherichia coli Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Gene Expression Regulation Genes, Bacterial Genetic engineering Genetic technics Genetic Vectors Methods. Procedures. Technologies Operon Oxygenases - genetics Plasmids promoters Pseudomonas - genetics Pseudomonas catechol 2,3-oxygenase Pseudomonas putida Recombinant DNA restriction endonucleases Streptomycin - pharmacology streptomycin resistance toluene degradation enzymes Vectors (cloning, transfer, expression). Insertion sequences and transposons
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!