CHARACTERIZATION OF MESSENGER RNA SPECIFIC FOR CARCINOEMBRYONIC ANTIGEN
Total poly(A)-containing RNA extracted from a rectal carcinoma was translated in a rabbit reticulocyte lysate. By addition of either a monoclonal or a polyclonal antibody, both monospecific for carcinoembryonic antigen, one protein with an apparent molecular weight of 85,000 was specifically precipi...
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| Veröffentlicht in: | Annals of the New York Academy of Sciences 1983-01, Vol.417 (1), p.21-30 |
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| container_title | Annals of the New York Academy of Sciences |
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| creator | Zimmermann, Wolfgang Friedrich, Roland Grunert, Friedrich Luckenbach, Gerd-Albrecht Thompson, John von Kleist, Sabine |
| description | Total poly(A)-containing RNA extracted from a rectal carcinoma was translated in a rabbit reticulocyte lysate. By addition of either a monoclonal or a polyclonal antibody, both monospecific for carcinoembryonic antigen, one protein with an apparent molecular weight of 85,000 was specifically precipitated, as shown by electrophoresis of the immunoprecipitate on sodium dodecylsulfate polyacrylamide gels. This protein behaves similarly to CEA isolated from liver metastases of a colon tumor in its property of being resistant to cleavage by cyanogen bromide. These findings suggest that this protein represents the CEA precursor protein. When we consider the high carbohydrate content of 60% of CEA, the observed molecular weight of the CEA precursor protein is in agreement with the reported molecular weight of 180,000 for CEA. By sedimentation of poly(A)-containing tumor RNA through a sucrose gradient, and by in vitro translation of each fraction of the gradient, the sedimentation coefficient of CEA-specific mRNA was found to be about 22 S. |
| doi_str_mv | 10.1111/j.1749-6632.1983.tb32844.x |
| format | Article |
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By addition of either a monoclonal or a polyclonal antibody, both monospecific for carcinoembryonic antigen, one protein with an apparent molecular weight of 85,000 was specifically precipitated, as shown by electrophoresis of the immunoprecipitate on sodium dodecylsulfate polyacrylamide gels. This protein behaves similarly to CEA isolated from liver metastases of a colon tumor in its property of being resistant to cleavage by cyanogen bromide. These findings suggest that this protein represents the CEA precursor protein. When we consider the high carbohydrate content of 60% of CEA, the observed molecular weight of the CEA precursor protein is in agreement with the reported molecular weight of 180,000 for CEA. By sedimentation of poly(A)-containing tumor RNA through a sucrose gradient, and by in vitro translation of each fraction of the gradient, the sedimentation coefficient of CEA-specific mRNA was found to be about 22 S.</description><identifier>ISSN: 0077-8923</identifier><identifier>EISSN: 1749-6632</identifier><identifier>DOI: 10.1111/j.1749-6632.1983.tb32844.x</identifier><identifier>PMID: 6200032</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Carcinoembryonic Antigen - genetics ; Centrifugation, Density Gradient ; Electrophoresis, Polyacrylamide Gel ; Humans ; Mice ; Mice, Inbred C3H ; Molecular Weight ; Poly A - isolation & purification ; Protein Biosynthesis ; Protein Precursors - analysis ; Rabbits ; Rectal Neoplasms - analysis ; RNA - isolation & purification ; RNA, Messenger - analysis</subject><ispartof>Annals of the New York Academy of Sciences, 1983-01, Vol.417 (1), p.21-30</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6200032$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zimmermann, Wolfgang</creatorcontrib><creatorcontrib>Friedrich, Roland</creatorcontrib><creatorcontrib>Grunert, Friedrich</creatorcontrib><creatorcontrib>Luckenbach, Gerd-Albrecht</creatorcontrib><creatorcontrib>Thompson, John</creatorcontrib><creatorcontrib>von Kleist, Sabine</creatorcontrib><title>CHARACTERIZATION OF MESSENGER RNA SPECIFIC FOR CARCINOEMBRYONIC ANTIGEN</title><title>Annals of the New York Academy of Sciences</title><addtitle>Ann N Y Acad Sci</addtitle><description>Total poly(A)-containing RNA extracted from a rectal carcinoma was translated in a rabbit reticulocyte lysate. By addition of either a monoclonal or a polyclonal antibody, both monospecific for carcinoembryonic antigen, one protein with an apparent molecular weight of 85,000 was specifically precipitated, as shown by electrophoresis of the immunoprecipitate on sodium dodecylsulfate polyacrylamide gels. This protein behaves similarly to CEA isolated from liver metastases of a colon tumor in its property of being resistant to cleavage by cyanogen bromide. These findings suggest that this protein represents the CEA precursor protein. When we consider the high carbohydrate content of 60% of CEA, the observed molecular weight of the CEA precursor protein is in agreement with the reported molecular weight of 180,000 for CEA. By sedimentation of poly(A)-containing tumor RNA through a sucrose gradient, and by in vitro translation of each fraction of the gradient, the sedimentation coefficient of CEA-specific mRNA was found to be about 22 S.</description><subject>Animals</subject><subject>Carcinoembryonic Antigen - genetics</subject><subject>Centrifugation, Density Gradient</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Humans</subject><subject>Mice</subject><subject>Mice, Inbred C3H</subject><subject>Molecular Weight</subject><subject>Poly A - isolation & purification</subject><subject>Protein Biosynthesis</subject><subject>Protein Precursors - analysis</subject><subject>Rabbits</subject><subject>Rectal Neoplasms - analysis</subject><subject>RNA - isolation & purification</subject><subject>RNA, Messenger - analysis</subject><issn>0077-8923</issn><issn>1749-6632</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9j1FPgzAUhRujmXP6E0yID76BpQXaPmItjGQrBjCLvjQFSsLc3ASWzH8vZov35STn-3KSC8CDCx13vKe14xKP2UGAkeMyip2hxIh6nnO8ANN_dAmmEBJiU4bwNbjp-zWE7qiRCZgECEKI0RTEfB5mIS9ElnyERZJKK42spchzIWORWZkMrfxV8CRKuBWlmcXDjCcyFcvn7D2VYxnKIomFvAVXjd705u6cM_AWiYLP7UUaJzxc2C3yvMHGpvGRr5mHDNa68kuPQkO0S2qGKhS4QU0YohUmFaGsKTWqA9IQTI0P2cgQnoHH0-6-230fTD-obdtXZrPRX2Z36BWFlBCf_on3Z_FQbk2t9l271d2POn8-cvvE234wx3-su08VEEx8tZKxiiTmL8E8Vyv8C-LMZPg</recordid><startdate>19830101</startdate><enddate>19830101</enddate><creator>Zimmermann, Wolfgang</creator><creator>Friedrich, Roland</creator><creator>Grunert, Friedrich</creator><creator>Luckenbach, Gerd-Albrecht</creator><creator>Thompson, John</creator><creator>von Kleist, Sabine</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19830101</creationdate><title>CHARACTERIZATION OF MESSENGER RNA SPECIFIC FOR CARCINOEMBRYONIC ANTIGEN</title><author>Zimmermann, Wolfgang ; Friedrich, Roland ; Grunert, Friedrich ; Luckenbach, Gerd-Albrecht ; Thompson, John ; von Kleist, Sabine</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i244t-3ef525a942e3aac5b480e7a17d92c2616d7928c37c789fba2d67f738e5096d723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Animals</topic><topic>Carcinoembryonic Antigen - genetics</topic><topic>Centrifugation, Density Gradient</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Humans</topic><topic>Mice</topic><topic>Mice, Inbred C3H</topic><topic>Molecular Weight</topic><topic>Poly A - isolation & purification</topic><topic>Protein Biosynthesis</topic><topic>Protein Precursors - analysis</topic><topic>Rabbits</topic><topic>Rectal Neoplasms - analysis</topic><topic>RNA - isolation & purification</topic><topic>RNA, Messenger - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zimmermann, Wolfgang</creatorcontrib><creatorcontrib>Friedrich, Roland</creatorcontrib><creatorcontrib>Grunert, Friedrich</creatorcontrib><creatorcontrib>Luckenbach, Gerd-Albrecht</creatorcontrib><creatorcontrib>Thompson, John</creatorcontrib><creatorcontrib>von Kleist, Sabine</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of the New York Academy of Sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zimmermann, Wolfgang</au><au>Friedrich, Roland</au><au>Grunert, Friedrich</au><au>Luckenbach, Gerd-Albrecht</au><au>Thompson, John</au><au>von Kleist, Sabine</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CHARACTERIZATION OF MESSENGER RNA SPECIFIC FOR CARCINOEMBRYONIC ANTIGEN</atitle><jtitle>Annals of the New York Academy of Sciences</jtitle><addtitle>Ann N Y Acad Sci</addtitle><date>1983-01-01</date><risdate>1983</risdate><volume>417</volume><issue>1</issue><spage>21</spage><epage>30</epage><pages>21-30</pages><issn>0077-8923</issn><eissn>1749-6632</eissn><abstract>Total poly(A)-containing RNA extracted from a rectal carcinoma was translated in a rabbit reticulocyte lysate. By addition of either a monoclonal or a polyclonal antibody, both monospecific for carcinoembryonic antigen, one protein with an apparent molecular weight of 85,000 was specifically precipitated, as shown by electrophoresis of the immunoprecipitate on sodium dodecylsulfate polyacrylamide gels. This protein behaves similarly to CEA isolated from liver metastases of a colon tumor in its property of being resistant to cleavage by cyanogen bromide. These findings suggest that this protein represents the CEA precursor protein. When we consider the high carbohydrate content of 60% of CEA, the observed molecular weight of the CEA precursor protein is in agreement with the reported molecular weight of 180,000 for CEA. By sedimentation of poly(A)-containing tumor RNA through a sucrose gradient, and by in vitro translation of each fraction of the gradient, the sedimentation coefficient of CEA-specific mRNA was found to be about 22 S.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>6200032</pmid><doi>10.1111/j.1749-6632.1983.tb32844.x</doi><tpages>10</tpages></addata></record> |
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| ispartof | Annals of the New York Academy of Sciences, 1983-01, Vol.417 (1), p.21-30 |
| issn | 0077-8923 1749-6632 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Carcinoembryonic Antigen - genetics Centrifugation, Density Gradient Electrophoresis, Polyacrylamide Gel Humans Mice Mice, Inbred C3H Molecular Weight Poly A - isolation & purification Protein Biosynthesis Protein Precursors - analysis Rabbits Rectal Neoplasms - analysis RNA - isolation & purification RNA, Messenger - analysis |
| title | CHARACTERIZATION OF MESSENGER RNA SPECIFIC FOR CARCINOEMBRYONIC ANTIGEN |
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