HUMAN GASTRIC CANCER-ASSOCIATED ANTIGENS AND CARCINOEMBRYONIC ANTIGEN DETECTED BY MONOCLONAL ANTIBODIES

The serologic and molecular characterization of four monoclonal antibodies to human gastric cancer cells and to CEA were described and the immunohistologic patterns of reactions with freshly obtained gastrointestinal tissues were examined. Monoclonal antibody YK004, which was secreted by the hybrido...

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Veröffentlicht in:Annals of the New York Academy of Sciences 1983-01, Vol.417 (1), p.158-168
Hauptverfasser: Yachi, Akira, Imai, Kohzoh, Moriya, Yasuo, Fujita, Hideo, Tanda, Mariko, Nishi, Shinzo, Kawaharada, Makoto
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container_title Annals of the New York Academy of Sciences
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creator Yachi, Akira
Imai, Kohzoh
Moriya, Yasuo
Fujita, Hideo
Tanda, Mariko
Nishi, Shinzo
Kawaharada, Makoto
description The serologic and molecular characterization of four monoclonal antibodies to human gastric cancer cells and to CEA were described and the immunohistologic patterns of reactions with freshly obtained gastrointestinal tissues were examined. Monoclonal antibody YK004, which was secreted by the hybridoma constructed with the gastric carcinoma cell line KATO III, reacted with an antigenic determinant preferentially expressed on human gastric and colonic carcinoma cells, but it failed to bind any nonmalignant tissues tested except the stomach. Although this antibody reacted with fetal digestive tract tissues, it failed to immunoprecipitate CEA. Monoclonal antibodies YK013 and YK024 prepared in the same manner by immunizing with the gastric carcinoma cell line KATO III reacted with antigenic determinants on CEA, but not on NCA or NCA2. The antigenic profiles recognized by these two antibodies were found to be identical by sequential immunoprecipitation experiments. However, the antigenic determinant recognized by antibody YK013 may be different from that recognized by antibody YK024, since the immunohistologic reactivity patterns in carcinoma tissue sections were clearly different. Monoclonal antibody AS001, which was secreted by the hybridomas constructed with purified CEA as immunogen, immunoprecipitated CEA molecule with a single antigenic structure of 200 Kd, which was different from the antigenic profile detected by two other monoclonal antibodies, YK013 and YK024. The unique distribution of the antigenic determinants recognized by our monoclonal antibodies on cancerous tissues suggests that antibodies might be able to detect the unique determinants of the circulating CEA molecule or CEA-related molecules in the serum of cancer patients.
doi_str_mv 10.1111/j.1749-6632.1983.tb32860.x
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Monoclonal antibody YK004, which was secreted by the hybridoma constructed with the gastric carcinoma cell line KATO III, reacted with an antigenic determinant preferentially expressed on human gastric and colonic carcinoma cells, but it failed to bind any nonmalignant tissues tested except the stomach. Although this antibody reacted with fetal digestive tract tissues, it failed to immunoprecipitate CEA. Monoclonal antibodies YK013 and YK024 prepared in the same manner by immunizing with the gastric carcinoma cell line KATO III reacted with antigenic determinants on CEA, but not on NCA or NCA2. The antigenic profiles recognized by these two antibodies were found to be identical by sequential immunoprecipitation experiments. However, the antigenic determinant recognized by antibody YK013 may be different from that recognized by antibody YK024, since the immunohistologic reactivity patterns in carcinoma tissue sections were clearly different. Monoclonal antibody AS001, which was secreted by the hybridomas constructed with purified CEA as immunogen, immunoprecipitated CEA molecule with a single antigenic structure of 200 Kd, which was different from the antigenic profile detected by two other monoclonal antibodies, YK013 and YK024. 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Monoclonal antibody YK004, which was secreted by the hybridoma constructed with the gastric carcinoma cell line KATO III, reacted with an antigenic determinant preferentially expressed on human gastric and colonic carcinoma cells, but it failed to bind any nonmalignant tissues tested except the stomach. Although this antibody reacted with fetal digestive tract tissues, it failed to immunoprecipitate CEA. Monoclonal antibodies YK013 and YK024 prepared in the same manner by immunizing with the gastric carcinoma cell line KATO III reacted with antigenic determinants on CEA, but not on NCA or NCA2. The antigenic profiles recognized by these two antibodies were found to be identical by sequential immunoprecipitation experiments. However, the antigenic determinant recognized by antibody YK013 may be different from that recognized by antibody YK024, since the immunohistologic reactivity patterns in carcinoma tissue sections were clearly different. Monoclonal antibody AS001, which was secreted by the hybridomas constructed with purified CEA as immunogen, immunoprecipitated CEA molecule with a single antigenic structure of 200 Kd, which was different from the antigenic profile detected by two other monoclonal antibodies, YK013 and YK024. The unique distribution of the antigenic determinants recognized by our monoclonal antibodies on cancerous tissues suggests that antibodies might be able to detect the unique determinants of the circulating CEA molecule or CEA-related molecules in the serum of cancer patients.</description><subject>Adenocarcinoma - analysis</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antigens, Neoplasm - analysis</subject><subject>Carcinoembryonic Antigen - analysis</subject><subject>Carcinoembryonic Antigen - immunology</subject><subject>Colon - analysis</subject><subject>Colonic Neoplasms - analysis</subject><subject>Epitopes - analysis</subject><subject>Female</subject><subject>Fetus - analysis</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Pregnancy</subject><subject>Stomach - analysis</subject><subject>Stomach Neoplasms - analysis</subject><issn>0077-8923</issn><issn>1749-6632</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1Pg0AQhjdGU2v1J5gQD97A_YDd5UgXpBi6JEBreiJAF4O2tgJN6r-XWtK5zLx5nkwmA8ATggbq6-XTQMy0dUoJNpDNidEVBHMKjeMVGF_QNRhDyJjObUxuwV3bfkKIMDfZCIwohhASOAYfs8XckZrvJGkcCE04Unix7iRJJAIn9VzNkWngezLpB7fHsQhk5M2n8SqSvT9QzfVST5z06UqbRzISYSSd8B9PIzfwkntwU-WbVj0MfQIWr14qZnoY-YFwQr3GptnpuCwtUrKCoaI_1IJWwXCRryFXplXZyKQlJYRUrMj7yJGFaYVZaa2ZQhUl3CIT8Hzeu292PwfVdtm2bku12eTfandoMw45o4icxMdBPBRbtc72Tb3Nm99s-EzP9TOv204dLzhvvjLKCLOyd-ln_vJNmtxdZiH5A0lGbWY</recordid><startdate>19830101</startdate><enddate>19830101</enddate><creator>Yachi, Akira</creator><creator>Imai, Kohzoh</creator><creator>Moriya, Yasuo</creator><creator>Fujita, Hideo</creator><creator>Tanda, Mariko</creator><creator>Nishi, Shinzo</creator><creator>Kawaharada, Makoto</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19830101</creationdate><title>HUMAN GASTRIC CANCER-ASSOCIATED ANTIGENS AND CARCINOEMBRYONIC ANTIGEN DETECTED BY MONOCLONAL ANTIBODIES</title><author>Yachi, Akira ; Imai, Kohzoh ; Moriya, Yasuo ; Fujita, Hideo ; Tanda, Mariko ; Nishi, Shinzo ; Kawaharada, Makoto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i244t-2cc53c7b71b847505b72bad08e45f9146c6333f7ba5f981526f27c5d7e1f63853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Adenocarcinoma - analysis</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antigens, Neoplasm - analysis</topic><topic>Carcinoembryonic Antigen - analysis</topic><topic>Carcinoembryonic Antigen - immunology</topic><topic>Colon - analysis</topic><topic>Colonic Neoplasms - analysis</topic><topic>Epitopes - analysis</topic><topic>Female</topic><topic>Fetus - analysis</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>Pregnancy</topic><topic>Stomach - analysis</topic><topic>Stomach Neoplasms - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yachi, Akira</creatorcontrib><creatorcontrib>Imai, Kohzoh</creatorcontrib><creatorcontrib>Moriya, Yasuo</creatorcontrib><creatorcontrib>Fujita, Hideo</creatorcontrib><creatorcontrib>Tanda, Mariko</creatorcontrib><creatorcontrib>Nishi, Shinzo</creatorcontrib><creatorcontrib>Kawaharada, Makoto</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of the New York Academy of Sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yachi, Akira</au><au>Imai, Kohzoh</au><au>Moriya, Yasuo</au><au>Fujita, Hideo</au><au>Tanda, Mariko</au><au>Nishi, Shinzo</au><au>Kawaharada, Makoto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>HUMAN GASTRIC CANCER-ASSOCIATED ANTIGENS AND CARCINOEMBRYONIC ANTIGEN DETECTED BY MONOCLONAL ANTIBODIES</atitle><jtitle>Annals of the New York Academy of Sciences</jtitle><addtitle>Ann N Y Acad Sci</addtitle><date>1983-01-01</date><risdate>1983</risdate><volume>417</volume><issue>1</issue><spage>158</spage><epage>168</epage><pages>158-168</pages><issn>0077-8923</issn><eissn>1749-6632</eissn><abstract>The serologic and molecular characterization of four monoclonal antibodies to human gastric cancer cells and to CEA were described and the immunohistologic patterns of reactions with freshly obtained gastrointestinal tissues were examined. Monoclonal antibody YK004, which was secreted by the hybridoma constructed with the gastric carcinoma cell line KATO III, reacted with an antigenic determinant preferentially expressed on human gastric and colonic carcinoma cells, but it failed to bind any nonmalignant tissues tested except the stomach. Although this antibody reacted with fetal digestive tract tissues, it failed to immunoprecipitate CEA. Monoclonal antibodies YK013 and YK024 prepared in the same manner by immunizing with the gastric carcinoma cell line KATO III reacted with antigenic determinants on CEA, but not on NCA or NCA2. The antigenic profiles recognized by these two antibodies were found to be identical by sequential immunoprecipitation experiments. However, the antigenic determinant recognized by antibody YK013 may be different from that recognized by antibody YK024, since the immunohistologic reactivity patterns in carcinoma tissue sections were clearly different. Monoclonal antibody AS001, which was secreted by the hybridomas constructed with purified CEA as immunogen, immunoprecipitated CEA molecule with a single antigenic structure of 200 Kd, which was different from the antigenic profile detected by two other monoclonal antibodies, YK013 and YK024. The unique distribution of the antigenic determinants recognized by our monoclonal antibodies on cancerous tissues suggests that antibodies might be able to detect the unique determinants of the circulating CEA molecule or CEA-related molecules in the serum of cancer patients.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>6200030</pmid><doi>10.1111/j.1749-6632.1983.tb32860.x</doi><tpages>11</tpages></addata></record>
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subjects Adenocarcinoma - analysis
Antibodies, Monoclonal - immunology
Antigens, Neoplasm - analysis
Carcinoembryonic Antigen - analysis
Carcinoembryonic Antigen - immunology
Colon - analysis
Colonic Neoplasms - analysis
Epitopes - analysis
Female
Fetus - analysis
Humans
Immunoenzyme Techniques
Pregnancy
Stomach - analysis
Stomach Neoplasms - analysis
title HUMAN GASTRIC CANCER-ASSOCIATED ANTIGENS AND CARCINOEMBRYONIC ANTIGEN DETECTED BY MONOCLONAL ANTIBODIES
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