Identification of subpopulations of T lymphocytes and Ia positive B lymphocytes using a rosette assay based upon the biotin-avidin interaction

Bovine erythrocytes were coated with avidin using a chromic chloride coupling technique and used successfully in an indirect rosette assay to identify and quantitate: (a) T-lymphocyte helper and suppressor subpopulations, and (b) Ia positive B-lymphocyte populations. Using mouse monoclonal antibodie...

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Veröffentlicht in:	Immunology letters 1983, Vol.7 (3), p.157-161
Hauptverfasser:	Mason, R.J., Stewart, K.N., Power, D.A., MacLeod, Alison M., Catto, G.R.D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis of the immune response. Humoral and cellular immunity Animals Antibodies, Monoclonal Avidin avidin coated ox erythrocytes B-Lymphocytes - classification B-Lymphocytes - immunology Biological and medical sciences Biotin biotinylated antibody Cattle chromic chloride Fundamental and applied biological sciences. Psychology Fundamental immunology Histocompatibility Antigens Class II HLA Antigens Humans Immunobiology Leukemia, Lymphoid - blood Leukemia, Lymphoid - immunology monoclonal antibodies Rosette Formation T-cell subsets T-Lymphocytes - classification T-Lymphocytes - immunology
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