Identification of subpopulations of T lymphocytes and Ia positive B lymphocytes using a rosette assay based upon the biotin-avidin interaction
Bovine erythrocytes were coated with avidin using a chromic chloride coupling technique and used successfully in an indirect rosette assay to identify and quantitate: (a) T-lymphocyte helper and suppressor subpopulations, and (b) Ia positive B-lymphocyte populations. Using mouse monoclonal antibodie...
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| Veröffentlicht in: | Immunology letters 1983, Vol.7 (3), p.157-161 |
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| container_title | Immunology letters |
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| creator | Mason, R.J. Stewart, K.N. Power, D.A. MacLeod, Alison M. Catto, G.R.D. |
| description | Bovine erythrocytes were coated with avidin using a chromic chloride coupling technique and used successfully in an indirect rosette assay to identify and quantitate: (a) T-lymphocyte helper and suppressor subpopulations, and (b) Ia positive B-lymphocyte populations.
Using mouse monoclonal antibodies to the T-lymphocyte markers OKT3, OKT4, and OKT8 it was shown that the proportion of OKT4 and OKT8 positive cells were respectively 60.9 and 39.2% of the total OKT-3 positive T lymphocytes. In a similar way, using a monoclonal anti-human Ia antibody, the percentage Ia positive cells in B-lymphocyte enriched preparations was shown to vary between 18 and 55% for normal peripheral blood and 31 and 58% for chronic lymphocytic leukaemia derived peripheral blood. |
| doi_str_mv | 10.1016/0165-2478(83)90064-0 |
| format | Article |
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Using mouse monoclonal antibodies to the T-lymphocyte markers OKT3, OKT4, and OKT8 it was shown that the proportion of OKT4 and OKT8 positive cells were respectively 60.9 and 39.2% of the total OKT-3 positive T lymphocytes. In a similar way, using a monoclonal anti-human Ia antibody, the percentage Ia positive cells in B-lymphocyte enriched preparations was shown to vary between 18 and 55% for normal peripheral blood and 31 and 58% for chronic lymphocytic leukaemia derived peripheral blood.</description><identifier>ISSN: 0165-2478</identifier><identifier>EISSN: 1879-0542</identifier><identifier>DOI: 10.1016/0165-2478(83)90064-0</identifier><identifier>PMID: 6607881</identifier><identifier>CODEN: IMLED6</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis of the immune response. Humoral and cellular immunity ; Animals ; Antibodies, Monoclonal ; Avidin ; avidin coated ox erythrocytes ; B-Lymphocytes - classification ; B-Lymphocytes - immunology ; Biological and medical sciences ; Biotin ; biotinylated antibody ; Cattle ; chromic chloride ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Histocompatibility Antigens Class II ; HLA Antigens ; Humans ; Immunobiology ; Leukemia, Lymphoid - blood ; Leukemia, Lymphoid - immunology ; monoclonal antibodies ; Rosette Formation ; T-cell subsets ; T-Lymphocytes - classification ; T-Lymphocytes - immunology</subject><ispartof>Immunology letters, 1983, Vol.7 (3), p.157-161</ispartof><rights>1983</rights><rights>1984 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c332t-df159dfcc7b7ce70d62ab3c7dcb97bb396578bacfa9acf2cc33fd2530bfdf7623</citedby><cites>FETCH-LOGICAL-c332t-df159dfcc7b7ce70d62ab3c7dcb97bb396578bacfa9acf2cc33fd2530bfdf7623</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0165247883900640$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9551181$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6607881$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mason, R.J.</creatorcontrib><creatorcontrib>Stewart, K.N.</creatorcontrib><creatorcontrib>Power, D.A.</creatorcontrib><creatorcontrib>MacLeod, Alison M.</creatorcontrib><creatorcontrib>Catto, G.R.D.</creatorcontrib><title>Identification of subpopulations of T lymphocytes and Ia positive B lymphocytes using a rosette assay based upon the biotin-avidin interaction</title><title>Immunology letters</title><addtitle>Immunol Lett</addtitle><description>Bovine erythrocytes were coated with avidin using a chromic chloride coupling technique and used successfully in an indirect rosette assay to identify and quantitate: (a) T-lymphocyte helper and suppressor subpopulations, and (b) Ia positive B-lymphocyte populations.
Using mouse monoclonal antibodies to the T-lymphocyte markers OKT3, OKT4, and OKT8 it was shown that the proportion of OKT4 and OKT8 positive cells were respectively 60.9 and 39.2% of the total OKT-3 positive T lymphocytes. In a similar way, using a monoclonal anti-human Ia antibody, the percentage Ia positive cells in B-lymphocyte enriched preparations was shown to vary between 18 and 55% for normal peripheral blood and 31 and 58% for chronic lymphocytic leukaemia derived peripheral blood.</description><subject>Analysis of the immune response. Humoral and cellular immunity</subject><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Avidin</subject><subject>avidin coated ox erythrocytes</subject><subject>B-Lymphocytes - classification</subject><subject>B-Lymphocytes - immunology</subject><subject>Biological and medical sciences</subject><subject>Biotin</subject><subject>biotinylated antibody</subject><subject>Cattle</subject><subject>chromic chloride</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Histocompatibility Antigens Class II</subject><subject>HLA Antigens</subject><subject>Humans</subject><subject>Immunobiology</subject><subject>Leukemia, Lymphoid - blood</subject><subject>Leukemia, Lymphoid - immunology</subject><subject>monoclonal antibodies</subject><subject>Rosette Formation</subject><subject>T-cell subsets</subject><subject>T-Lymphocytes - classification</subject><subject>T-Lymphocytes - immunology</subject><issn>0165-2478</issn><issn>1879-0542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2KFDEUhYMoYzv6BgpZiOiiNKlUVVIbQQd_GgbcjOuQnxsnUp2UdVMN_RI-s1XdTYMbXSSBe757bpJDyHPO3nLGu3fLaqu6keq1Em96xrqmYg_IhivZV6xt6odkc0EekyeIPxnjrWjEFbnqOiaV4hvye-shlRiiMyXmRHOgONsxj_NwLOBauaPDYTfeZ3cogNQkT7eGjhljiXugH_9SZ4zpBzV0ygilADWI5kCtQfB0HpcJ5R6ojbnEVJl99DHRmApMxq3jnpJHwQwIz87nNfn--dPdzdfq9tuX7c2H28oJUZfKB972PjgnrXQgme9qY4WT3tleWiv6rpXKGhdMv2y1W7qCr1vBbPBBdrW4Jq9OvuOUf82ARe8iOhgGkyDPqBVTUjSK_xfkomdtfXRsTqBbXo4TBD1OcWemg-ZMr3npNQy9hqGV0Me8NFvaXpz9Z7sDf2k6B7ToL8-6QWeGMJnkIl6wvm05P2LvTxgsn7aPMGl0EZIDHydwRfsc_32PP_tdtbI</recordid><startdate>1983</startdate><enddate>1983</enddate><creator>Mason, R.J.</creator><creator>Stewart, K.N.</creator><creator>Power, D.A.</creator><creator>MacLeod, Alison M.</creator><creator>Catto, G.R.D.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>1983</creationdate><title>Identification of subpopulations of T lymphocytes and Ia positive B lymphocytes using a rosette assay based upon the biotin-avidin interaction</title><author>Mason, R.J. ; Stewart, K.N. ; Power, D.A. ; MacLeod, Alison M. ; Catto, G.R.D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-df159dfcc7b7ce70d62ab3c7dcb97bb396578bacfa9acf2cc33fd2530bfdf7623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Analysis of the immune response. Humoral and cellular immunity</topic><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Avidin</topic><topic>avidin coated ox erythrocytes</topic><topic>B-Lymphocytes - classification</topic><topic>B-Lymphocytes - immunology</topic><topic>Biological and medical sciences</topic><topic>Biotin</topic><topic>biotinylated antibody</topic><topic>Cattle</topic><topic>chromic chloride</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Histocompatibility Antigens Class II</topic><topic>HLA Antigens</topic><topic>Humans</topic><topic>Immunobiology</topic><topic>Leukemia, Lymphoid - blood</topic><topic>Leukemia, Lymphoid - immunology</topic><topic>monoclonal antibodies</topic><topic>Rosette Formation</topic><topic>T-cell subsets</topic><topic>T-Lymphocytes - classification</topic><topic>T-Lymphocytes - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mason, R.J.</creatorcontrib><creatorcontrib>Stewart, K.N.</creatorcontrib><creatorcontrib>Power, D.A.</creatorcontrib><creatorcontrib>MacLeod, Alison M.</creatorcontrib><creatorcontrib>Catto, G.R.D.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Immunology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mason, R.J.</au><au>Stewart, K.N.</au><au>Power, D.A.</au><au>MacLeod, Alison M.</au><au>Catto, G.R.D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of subpopulations of T lymphocytes and Ia positive B lymphocytes using a rosette assay based upon the biotin-avidin interaction</atitle><jtitle>Immunology letters</jtitle><addtitle>Immunol Lett</addtitle><date>1983</date><risdate>1983</risdate><volume>7</volume><issue>3</issue><spage>157</spage><epage>161</epage><pages>157-161</pages><issn>0165-2478</issn><eissn>1879-0542</eissn><coden>IMLED6</coden><abstract>Bovine erythrocytes were coated with avidin using a chromic chloride coupling technique and used successfully in an indirect rosette assay to identify and quantitate: (a) T-lymphocyte helper and suppressor subpopulations, and (b) Ia positive B-lymphocyte populations.
Using mouse monoclonal antibodies to the T-lymphocyte markers OKT3, OKT4, and OKT8 it was shown that the proportion of OKT4 and OKT8 positive cells were respectively 60.9 and 39.2% of the total OKT-3 positive T lymphocytes. In a similar way, using a monoclonal anti-human Ia antibody, the percentage Ia positive cells in B-lymphocyte enriched preparations was shown to vary between 18 and 55% for normal peripheral blood and 31 and 58% for chronic lymphocytic leukaemia derived peripheral blood.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>6607881</pmid><doi>10.1016/0165-2478(83)90064-0</doi><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 0165-2478 |
| ispartof | Immunology letters, 1983, Vol.7 (3), p.157-161 |
| issn | 0165-2478 1879-0542 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Analysis of the immune response. Humoral and cellular immunity Animals Antibodies, Monoclonal Avidin avidin coated ox erythrocytes B-Lymphocytes - classification B-Lymphocytes - immunology Biological and medical sciences Biotin biotinylated antibody Cattle chromic chloride Fundamental and applied biological sciences. Psychology Fundamental immunology Histocompatibility Antigens Class II HLA Antigens Humans Immunobiology Leukemia, Lymphoid - blood Leukemia, Lymphoid - immunology monoclonal antibodies Rosette Formation T-cell subsets T-Lymphocytes - classification T-Lymphocytes - immunology |
| title | Identification of subpopulations of T lymphocytes and Ia positive B lymphocytes using a rosette assay based upon the biotin-avidin interaction |
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