Measurement of the bacterial nitrogen entering the duodenum of the ruminant using diaminopimelic acid as a marker
1. A technique for the separation and colorimetric estimation of 2, 4-diaminopimelic acid (DAP) using automated ion-exchange chromatography coupled with an acid ninhydrin detection system is described. 2. Only traces of DAP were found in rumen protozoa and no DAP was detected in rumen fluid prepared...
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| Veröffentlicht in: | British journal of nutrition 1971-01, Vol.25 (1), p.165-173 |
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| container_end_page | 173 |
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| container_title | British journal of nutrition |
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| creator | Hutton, K. Bailey, F. J. Annison, E. F. |
| description | 1. A technique for the separation and colorimetric estimation of 2, 4-diaminopimelic acid (DAP) using automated ion-exchange chromatography coupled with an acid ninhydrin detection system is described. 2. Only traces of DAP were found in rumen protozoa and no DAP was detected in rumen fluid prepared by ultracentrifugation or dialysis. 3. The concentration of DAP in rumen bacteria from sheep on a constant feeding regimen, and the ratio of nitrogen to DAP for these bacteria were found to be constant over a 3-month period. 4. The method has proved suitable for the estimation of bacterial N in the duodenal digesta of ruminants. 5. The contribution of bacterial N to the total N leaving the abomasum of a lactating cow fitted with a permanent re-entrant cannula in the duodenum was found to be 50%. |
| doi_str_mv | 10.1079/BJN19710074 |
| format | Article |
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J. ; Annison, E. F.</creator><creatorcontrib>Hutton, K. ; Bailey, F. J. ; Annison, E. F.</creatorcontrib><description>1. A technique for the separation and colorimetric estimation of 2, 4-diaminopimelic acid (DAP) using automated ion-exchange chromatography coupled with an acid ninhydrin detection system is described. 2. Only traces of DAP were found in rumen protozoa and no DAP was detected in rumen fluid prepared by ultracentrifugation or dialysis. 3. The concentration of DAP in rumen bacteria from sheep on a constant feeding regimen, and the ratio of nitrogen to DAP for these bacteria were found to be constant over a 3-month period. 4. The method has proved suitable for the estimation of bacterial N in the duodenal digesta of ruminants. 5. The contribution of bacterial N to the total N leaving the abomasum of a lactating cow fitted with a permanent re-entrant cannula in the duodenum was found to be 50%.</description><identifier>ISSN: 0007-1145</identifier><identifier>EISSN: 1475-2662</identifier><identifier>DOI: 10.1079/BJN19710074</identifier><identifier>PMID: 5099652</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Amino Acids - analysis ; Animals ; Bacteria - metabolism ; Bacterial Proteins - analysis ; Cattle ; Chromatography, Ion Exchange ; Duodenum - analysis ; Female ; Indicators and Reagents ; Lactation ; Nitrogen - analysis ; Pimelic Acids - analysis ; Pregnancy ; Rumen - microbiology ; Sheep</subject><ispartof>British journal of nutrition, 1971-01, Vol.25 (1), p.165-173</ispartof><rights>Copyright © The Nutrition Society 1971</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c397t-aa3204943b85a2aa7f6da442f821deab0238115fd4ae26d616c620d22bb48d9c3</citedby><cites>FETCH-LOGICAL-c397t-aa3204943b85a2aa7f6da442f821deab0238115fd4ae26d616c620d22bb48d9c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5099652$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hutton, K.</creatorcontrib><creatorcontrib>Bailey, F. J.</creatorcontrib><creatorcontrib>Annison, E. F.</creatorcontrib><title>Measurement of the bacterial nitrogen entering the duodenum of the ruminant using diaminopimelic acid as a marker</title><title>British journal of nutrition</title><addtitle>Br J Nutr</addtitle><description>1. A technique for the separation and colorimetric estimation of 2, 4-diaminopimelic acid (DAP) using automated ion-exchange chromatography coupled with an acid ninhydrin detection system is described. 2. Only traces of DAP were found in rumen protozoa and no DAP was detected in rumen fluid prepared by ultracentrifugation or dialysis. 3. The concentration of DAP in rumen bacteria from sheep on a constant feeding regimen, and the ratio of nitrogen to DAP for these bacteria were found to be constant over a 3-month period. 4. The method has proved suitable for the estimation of bacterial N in the duodenal digesta of ruminants. 5. The contribution of bacterial N to the total N leaving the abomasum of a lactating cow fitted with a permanent re-entrant cannula in the duodenum was found to be 50%.</description><subject>Amino Acids - analysis</subject><subject>Animals</subject><subject>Bacteria - metabolism</subject><subject>Bacterial Proteins - analysis</subject><subject>Cattle</subject><subject>Chromatography, Ion Exchange</subject><subject>Duodenum - analysis</subject><subject>Female</subject><subject>Indicators and Reagents</subject><subject>Lactation</subject><subject>Nitrogen - analysis</subject><subject>Pimelic Acids - analysis</subject><subject>Pregnancy</subject><subject>Rumen - microbiology</subject><subject>Sheep</subject><issn>0007-1145</issn><issn>1475-2662</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1971</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkM9vFSEQx4nR1Gf15NmEkxezCiwLy1EbrZpnG7WNRzILs6-0--MVlsT-96V9z-rBE5n5fJiBLyEvOXvLmTbvPnw94UZzxrR8RFZc6qYSSonHZMVKr-JcNk_Js5QuS9lyZg7IQcOMUY1YketvCClHHHFa6NzT5QJpB27BGGCgU1jivMGJFlo60-ae-zx7nPL4x495DBOU-zndKT5AqedtGHEIjoILnkKiQEeIVxifkyc9DAlf7M9Dcv7p49nR52p9evzl6P26crXRSwVQCyaNrLu2AQGge-VBStG3gnuEjom65bzpvQQUyiuunBLMC9F1svXG1Yfk9W7uNs7XGdNix5AcDgNMOOdkW9Y2rTSmiG92ootzShF7u42hvPXGcmbvArb_BFzsV_uxuRvRP7j7RAuvdjykBX8_4PJ1q3StG6uOv9ufP4Ra61_Knv31HYxdDH6D9nLOcSrR_Hf_LTBolGE</recordid><startdate>19710101</startdate><enddate>19710101</enddate><creator>Hutton, K.</creator><creator>Bailey, F. 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The concentration of DAP in rumen bacteria from sheep on a constant feeding regimen, and the ratio of nitrogen to DAP for these bacteria were found to be constant over a 3-month period. 4. The method has proved suitable for the estimation of bacterial N in the duodenal digesta of ruminants. 5. The contribution of bacterial N to the total N leaving the abomasum of a lactating cow fitted with a permanent re-entrant cannula in the duodenum was found to be 50%.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>5099652</pmid><doi>10.1079/BJN19710074</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | British journal of nutrition, 1971-01, Vol.25 (1), p.165-173 |
| issn | 0007-1145 1475-2662 |
| language | eng |
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| source | Electronic Journals Library; MEDLINE; Free Full-Text Journals in Chemistry |
| subjects | Amino Acids - analysis Animals Bacteria - metabolism Bacterial Proteins - analysis Cattle Chromatography, Ion Exchange Duodenum - analysis Female Indicators and Reagents Lactation Nitrogen - analysis Pimelic Acids - analysis Pregnancy Rumen - microbiology Sheep |
| title | Measurement of the bacterial nitrogen entering the duodenum of the ruminant using diaminopimelic acid as a marker |
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