Human Blastocysts grown in Culture
WE have already described the culture of cleaving human embryos to the sixteen celled stage 1 , and we now wish to give details of a few embryos that have developed much further, including two that reached fully developed blastocysts. Methods were similar to those described before. Preovulatory oocy...
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| Veröffentlicht in: | Nature (London) 1971-01, Vol.229 (5280), p.132-133 |
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| container_title | Nature (London) |
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| creator | STEPTOE, P. C. EDWARDS, R. G. PURDY, J. M. |
| description | WE have already described the culture of cleaving human embryos to the sixteen celled stage
1
, and we now wish to give details of a few embryos that have developed much further, including two that reached fully developed blastocysts. Methods were similar to those described before. Preovulatory oocytes recovered by laparoscopy
2
were fertilized in Bavister's medium
3
, and transferred after 12–15 h into Ham's F 10 supplemented with human or foetal calf serum. Preliminary details of this work have been presented elsewhere
4
. |
| doi_str_mv | 10.1038/229132a0 |
| format | Article |
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1
, and we now wish to give details of a few embryos that have developed much further, including two that reached fully developed blastocysts. Methods were similar to those described before. Preovulatory oocytes recovered by laparoscopy
2
were fertilized in Bavister's medium
3
, and transferred after 12–15 h into Ham's F 10 supplemented with human or foetal calf serum. Preliminary details of this work have been presented elsewhere
4
.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/229132a0</identifier><identifier>PMID: 4923103</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Culture Media ; Culture Techniques ; Female ; Humanities and Social Sciences ; Humans ; letter ; Mitosis ; multidisciplinary ; Ovum ; Science ; Science (multidisciplinary)</subject><ispartof>Nature (London), 1971-01, Vol.229 (5280), p.132-133</ispartof><rights>Springer Nature Limited 1971</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c312t-27e3f4b11be4866519c7d9a6b461499bf508d5bd839a547b2bd57ca803860b2f3</citedby><cites>FETCH-LOGICAL-c312t-27e3f4b11be4866519c7d9a6b461499bf508d5bd839a547b2bd57ca803860b2f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/229132a0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/229132a0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4923103$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>STEPTOE, P. C.</creatorcontrib><creatorcontrib>EDWARDS, R. G.</creatorcontrib><creatorcontrib>PURDY, J. M.</creatorcontrib><title>Human Blastocysts grown in Culture</title><title>Nature (London)</title><addtitle>Nature</addtitle><addtitle>Nature</addtitle><description>WE have already described the culture of cleaving human embryos to the sixteen celled stage
1
, and we now wish to give details of a few embryos that have developed much further, including two that reached fully developed blastocysts. Methods were similar to those described before. Preovulatory oocytes recovered by laparoscopy
2
were fertilized in Bavister's medium
3
, and transferred after 12–15 h into Ham's F 10 supplemented with human or foetal calf serum. Preliminary details of this work have been presented elsewhere
4
.</description><subject>Culture Media</subject><subject>Culture Techniques</subject><subject>Female</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>letter</subject><subject>Mitosis</subject><subject>multidisciplinary</subject><subject>Ovum</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><issn>0028-0836</issn><issn>1476-4687</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1971</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNplkEtLAzEUhYMotVbBPyAMLkQXozfvZKlFrVBwo-uQzGRKyzw0mSD990Za3bi6i_Odwz0HoXMMtxiouiNEY0osHKApZlKUTCh5iKYARJWgqDhGJzFuAIBjySZowjSh2ThFl4vU2b54aG0ch2obx1iswvDVF-u-mKd2TMGfoqPGttGf7e8MvT89vs0X5fL1-WV-vywrislYEulpwxzGzjMlBMe6krW2wjGBmdau4aBq7mpFteVMOuJqLiur8vsCHGnoDF3tcj_C8Jl8HE23jpVvW9v7IUWjQHGpOMng9Q6swhBj8I35COvOhq3BYH7mML9zZPRin5lc5-s_cN8_6zc7PWalX_lgNkMKfa75P-sbfutk2A</recordid><startdate>19710108</startdate><enddate>19710108</enddate><creator>STEPTOE, P. C.</creator><creator>EDWARDS, R. G.</creator><creator>PURDY, J. M.</creator><general>Nature Publishing Group UK</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19710108</creationdate><title>Human Blastocysts grown in Culture</title><author>STEPTOE, P. C. ; EDWARDS, R. G. ; PURDY, J. M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c312t-27e3f4b11be4866519c7d9a6b461499bf508d5bd839a547b2bd57ca803860b2f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1971</creationdate><topic>Culture Media</topic><topic>Culture Techniques</topic><topic>Female</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>letter</topic><topic>Mitosis</topic><topic>multidisciplinary</topic><topic>Ovum</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>STEPTOE, P. C.</creatorcontrib><creatorcontrib>EDWARDS, R. G.</creatorcontrib><creatorcontrib>PURDY, J. M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Nature (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>STEPTOE, P. C.</au><au>EDWARDS, R. G.</au><au>PURDY, J. M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human Blastocysts grown in Culture</atitle><jtitle>Nature (London)</jtitle><stitle>Nature</stitle><addtitle>Nature</addtitle><date>1971-01-08</date><risdate>1971</risdate><volume>229</volume><issue>5280</issue><spage>132</spage><epage>133</epage><pages>132-133</pages><issn>0028-0836</issn><eissn>1476-4687</eissn><abstract>WE have already described the culture of cleaving human embryos to the sixteen celled stage
1
, and we now wish to give details of a few embryos that have developed much further, including two that reached fully developed blastocysts. Methods were similar to those described before. Preovulatory oocytes recovered by laparoscopy
2
were fertilized in Bavister's medium
3
, and transferred after 12–15 h into Ham's F 10 supplemented with human or foetal calf serum. Preliminary details of this work have been presented elsewhere
4
.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>4923103</pmid><doi>10.1038/229132a0</doi><tpages>2</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0028-0836 |
| ispartof | Nature (London), 1971-01, Vol.229 (5280), p.132-133 |
| issn | 0028-0836 1476-4687 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80857852 |
| source | MEDLINE; SpringerLink Journals; Nature Journals Online |
| subjects | Culture Media Culture Techniques Female Humanities and Social Sciences Humans letter Mitosis multidisciplinary Ovum Science Science (multidisciplinary) |
| title | Human Blastocysts grown in Culture |
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