Cloning and expression in Escherichia coli of full-length complementary DNA coding for human alpha 1-antitrypsin

A cDNA library prepared from human liver was screened for alpha 1-antitrypsin, a major constituent of plasma which functions as inhibitor of proteolytic enzymes. The library was screened using a 12-base-long synthetic oligodeoxyribonucleotide corresponding to a known DNA fragment of human alpha 1-an...

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Veröffentlicht in:	DNA (New York, N.Y.) N.Y.), 1983-01, Vol.2 (4), p.255-264
Hauptverfasser:	Bollen, A, Herzog, A, Cravador, A, Hérion, P, Chuchana, P, Vander Straten, A, Loriau, R, Jacobs, P, van Elsen, A
Format:	Artikel
Sprache:	eng
Schlagworte:	alpha 1-Antitrypsin - genetics Base Sequence Cloning, Molecular DNA - genetics DNA Restriction Enzymes DNA, Bacterial - genetics DNA, Recombinant Escherichia coli - genetics Humans Plasmids RNA, Messenger - genetics
Online-Zugang:	Volltext
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