A quick method of demonstrating bacillaemia in patients with lepromatous leprosy and ultrastructural studies of the circulating acid-fast bacilli
While studying circulating immune complexes (CIC) in the sera of lepromatous patients by the polyethylene glycol (PEG) precipitation technique, we found (by light microscopy) abundant acid-fast bacilli (AFB), morphologically similar to those seen in slit skin smear preparations from these patients,...
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| Veröffentlicht in: | Transactions of the Royal Society of Tropical Medicine and Hygiene 1983, Vol.77 (5), p.660-664 |
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| container_title | Transactions of the Royal Society of Tropical Medicine and Hygiene |
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| creator | Saha, Kunal Chakraborty, Asit K. Prakash, Nam |
| description | While studying circulating immune complexes (CIC) in the sera of lepromatous patients by the polyethylene glycol (PEG) precipitation technique, we found (by light microscopy) abundant acid-fast bacilli (AFB), morphologically similar to those seen in slit skin smear preparations from these patients, precipitated with the PEG precipitated materials. Both solid and non-solid AFB could be readily identified. Ultrastructures of these AFB in the PEG aggregates showed some similarity with those detected in the PEG precipitates prepared from armadillo-derived lepromin under identical conditions. The most striking difference between the AFB in the test sera and that in the armadillo-derived lepromin was the absence of any transverse band in the former. This suggested that the AFB in the patients' circulation were not in division stage. Furthermore, electron-dense material were deposited on the AFB co-precipitated from the patients' sera, which were not found on the AFB co-precipitated from the armadillo-derived lepromin. |
| doi_str_mv | 10.1016/0035-9203(83)90196-7 |
| format | Article |
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Both solid and non-solid AFB could be readily identified. Ultrastructures of these AFB in the PEG aggregates showed some similarity with those detected in the PEG precipitates prepared from armadillo-derived lepromin under identical conditions. The most striking difference between the AFB in the test sera and that in the armadillo-derived lepromin was the absence of any transverse band in the former. This suggested that the AFB in the patients' circulation were not in division stage. 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Both solid and non-solid AFB could be readily identified. Ultrastructures of these AFB in the PEG aggregates showed some similarity with those detected in the PEG precipitates prepared from armadillo-derived lepromin under identical conditions. The most striking difference between the AFB in the test sera and that in the armadillo-derived lepromin was the absence of any transverse band in the former. This suggested that the AFB in the patients' circulation were not in division stage. Furthermore, electron-dense material were deposited on the AFB co-precipitated from the patients' sera, which were not found on the AFB co-precipitated from the armadillo-derived lepromin.</description><subject>Adult</subject><subject>Aged</subject><subject>Animals</subject><subject>Armadillos</subject><subject>Bacteriological Techniques</subject><subject>Blood - microbiology</subject><subject>Chemical Precipitation</subject><subject>Humans</subject><subject>Lepromin</subject><subject>Leprosy - microbiology</subject><subject>Microscopy, Electron</subject><subject>Middle Aged</subject><subject>Mycobacterium leprae - isolation & purification</subject><subject>Mycobacterium leprae - ultrastructure</subject><subject>Polyethylene Glycols</subject><subject>Sepsis - microbiology</subject><issn>0035-9203</issn><issn>1878-3503</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UctuFDEQtBAoLIE_AMknFA4DfqzHngtStIIEKQoHHoq4WB67zZrMY-MHkM_gj_Eyqz1ystVdVd1VjdBzSl5TQts3hHDRdIzwM8VfdYR2bSMfoBVVUjVcEP4QrY6Qx-hJSj8IYYKK7gSdtLxllHYr9Occ35Vgb_EIeTs7PHvsYJynlKPJYfqOe2PDMBgYg8FhwrtahSkn_CvkLR5gF-fR5Lmk5Z_usZkcLkOlV4lic4lmwCkXFyDt1fMWsA3RlmHRr_Ku8RV8mBSeokfeDAmeHd5T9OX9u8-by-bq48WHzflVY5nociOhX3tliSWU9JJJRj3UAu09MU4xJjxdK2ONtEZxJVRLJe8NOE_BKmEYP0UvF9269l2BlPUYkoXqdYLqRyuiOCdCVeB6AdrqL0XwehfDaOK9pkTvL6H3Met9zFpx_e8SWlbai4N-6UdwR9Ih-tpvln5IGX4f2ybe6lZyKfTlzTd9_enr5kZeXGtS8W8XPNRQfgaIOtl6CgsuRLBZuzn8f6G_oWerTA</recordid><startdate>1983</startdate><enddate>1983</enddate><creator>Saha, Kunal</creator><creator>Chakraborty, Asit K.</creator><creator>Prakash, Nam</creator><general>Elsevier Ltd</general><general>Royal Society of Tropical Medicine and Hygiene</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1983</creationdate><title>A quick method of demonstrating bacillaemia in patients with lepromatous leprosy and ultrastructural studies of the circulating acid-fast bacilli</title><author>Saha, Kunal ; Chakraborty, Asit K. ; Prakash, Nam</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c259t-7eb4f8c0c010b72721fe4f81bf0ad8225f148aca7ca838586173baedf1ec85a23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Animals</topic><topic>Armadillos</topic><topic>Bacteriological Techniques</topic><topic>Blood - microbiology</topic><topic>Chemical Precipitation</topic><topic>Humans</topic><topic>Lepromin</topic><topic>Leprosy - microbiology</topic><topic>Microscopy, Electron</topic><topic>Middle Aged</topic><topic>Mycobacterium leprae - isolation & purification</topic><topic>Mycobacterium leprae - ultrastructure</topic><topic>Polyethylene Glycols</topic><topic>Sepsis - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saha, Kunal</creatorcontrib><creatorcontrib>Chakraborty, Asit K.</creatorcontrib><creatorcontrib>Prakash, Nam</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Transactions of the Royal Society of Tropical Medicine and Hygiene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saha, Kunal</au><au>Chakraborty, Asit K.</au><au>Prakash, Nam</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A quick method of demonstrating bacillaemia in patients with lepromatous leprosy and ultrastructural studies of the circulating acid-fast bacilli</atitle><jtitle>Transactions of the Royal Society of Tropical Medicine and Hygiene</jtitle><addtitle>Trans R Soc Trop Med Hyg</addtitle><date>1983</date><risdate>1983</risdate><volume>77</volume><issue>5</issue><spage>660</spage><epage>664</epage><pages>660-664</pages><issn>0035-9203</issn><eissn>1878-3503</eissn><abstract>While studying circulating immune complexes (CIC) in the sera of lepromatous patients by the polyethylene glycol (PEG) precipitation technique, we found (by light microscopy) abundant acid-fast bacilli (AFB), morphologically similar to those seen in slit skin smear preparations from these patients, precipitated with the PEG precipitated materials. Both solid and non-solid AFB could be readily identified. Ultrastructures of these AFB in the PEG aggregates showed some similarity with those detected in the PEG precipitates prepared from armadillo-derived lepromin under identical conditions. The most striking difference between the AFB in the test sera and that in the armadillo-derived lepromin was the absence of any transverse band in the former. This suggested that the AFB in the patients' circulation were not in division stage. Furthermore, electron-dense material were deposited on the AFB co-precipitated from the patients' sera, which were not found on the AFB co-precipitated from the armadillo-derived lepromin.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>6362119</pmid><doi>10.1016/0035-9203(83)90196-7</doi><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 0035-9203 |
| ispartof | Transactions of the Royal Society of Tropical Medicine and Hygiene, 1983, Vol.77 (5), p.660-664 |
| issn | 0035-9203 1878-3503 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Adult Aged Animals Armadillos Bacteriological Techniques Blood - microbiology Chemical Precipitation Humans Lepromin Leprosy - microbiology Microscopy, Electron Middle Aged Mycobacterium leprae - isolation & purification Mycobacterium leprae - ultrastructure Polyethylene Glycols Sepsis - microbiology |
| title | A quick method of demonstrating bacillaemia in patients with lepromatous leprosy and ultrastructural studies of the circulating acid-fast bacilli |
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