[56] In vitro processing of plant preproteins

This chapter proposes to develop a cell-free system for the translation, processing and packaging of plant preproteins that demonstrates the synthesis, processing, and deposition of zein polypeptides and α-amylase. The method uses a nuclease-treated rough endoplasmic reticulum (RER)-enriched prepara...

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Veröffentlicht in:	Methods in Enzymology 1983, Vol.96, p.716-721
Hauptverfasser:	Burr, F.A., Burr, B.
Format:	Artikel
Sprache:	eng
Schlagworte:	Electrophoresis, Polyacrylamide Gel - methods Fluorometry - methods Membrane Proteins - genetics Membrane Proteins - isolation & purification Membranes - metabolism Membranes - ultrastructure Plant Proteins - genetics Plant Proteins - isolation & purification Plants - metabolism Protein Precursors - genetics Protein Processing, Post-Translational
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