Protuberic Acid-Hydrolytic Enzyme in Kobayasia nipponica and Characterization of the Hydrolytic Products
When the cold water-extract of Kobayasia nipponica containing a glycuronan, protuberic acid (PA), was allowed to stand at room temperature, PA was hydrolyzed. The optimum conditions for this PA-hydrolysis were 37°C and pH 4–5 in 0.1 M acetate buffer. Characterization of the hydrolytic products was p...
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| Veröffentlicht in: | Journal of biochemistry (Tokyo) 1983-10, Vol.94 (4), p.1037-1044 |
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| container_end_page | 1044 |
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| container_issue | 4 |
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| container_title | Journal of biochemistry (Tokyo) |
| container_volume | 94 |
| creator | TSUCHIHASHI, Hiroshi YADOMAE, Toshiro MIYAZAKI, Toshio |
| description | When the cold water-extract of Kobayasia nipponica containing a glycuronan, protuberic acid (PA), was allowed to stand at room temperature, PA was hydrolyzed. The optimum conditions for this PA-hydrolysis were 37°C and pH 4–5 in 0.1 M acetate buffer. Characterization of the hydrolytic products was performed by chemical analysis, and by 1H- and 13C-NMR spectroscopy. They were identified as D-GlcUA and O-(α-L-IdUAp)-(l-4)-D-GlcUA. These results suggest that PA-hydrolytic enzyme(s) include at least endo-β-D-glucuronidase. |
| doi_str_mv | 10.1093/oxfordjournals.jbchem.a134445 |
| format | Article |
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The optimum conditions for this PA-hydrolysis were 37°C and pH 4–5 in 0.1 M acetate buffer. Characterization of the hydrolytic products was performed by chemical analysis, and by 1H- and 13C-NMR spectroscopy. They were identified as D-GlcUA and O-(α-L-IdUAp)-(l-4)-D-GlcUA. 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These results suggest that PA-hydrolytic enzyme(s) include at least endo-β-D-glucuronidase.</description><subject>Basidiomycota - enzymology</subject><subject>Carbohydrate Sequence</subject><subject>Glucuronidase - metabolism</subject><subject>Hydrolysis</subject><subject>Kinetics</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Polysaccharides - isolation & purification</subject><issn>0021-924X</issn><issn>1756-2651</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkEtLHEEQxxtJ0I36EYS-JLfZ9HMeBw-yqJvEaJAExEtT0w-2153ptbsHHD99RnaR5FQU_0cVP4Q-UzKnpOFfw4sL0azDEHvYpPm61SvbzYFyIYQ8QDNaybJgpaQf0IwQRouGiYcj9Cml9dvKOD9Eh2UpRS34DK1-xZCH1kav8YX2pliOJobNmKf9sn8dO4t9j3-EFkZIHnDvt9vQew0YeoMXK4ig85R-hexDj4PDeWXxPyVTvxl0Tifoo5v-taf7eYz-XF3-XiyLm7vrb4uLm0ILWebCEimck9BQcBW1peOUVKKmLaUNrSmQpnZVS3jNa0NY1XAJzghDpK2MYJP9GH3Z9W5jeB5syqrzSdvNBnobhqRqUlMmBZuM5zujjiGlaJ3aRt9BHBUl6o20-p-02pFWe9JT_mx_aGg7a97Te7STXux0n7J9eZchPqmy4pVUy4dHdft9yZqf90w98r_mtZK8</recordid><startdate>198310</startdate><enddate>198310</enddate><creator>TSUCHIHASHI, Hiroshi</creator><creator>YADOMAE, Toshiro</creator><creator>MIYAZAKI, Toshio</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198310</creationdate><title>Protuberic Acid-Hydrolytic Enzyme in Kobayasia nipponica and Characterization of the Hydrolytic Products</title><author>TSUCHIHASHI, Hiroshi ; YADOMAE, Toshiro ; MIYAZAKI, Toshio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-e054ff5a91af71e6f3107481b119181a098f7b03838d027935afd4d05e7d426f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Basidiomycota - enzymology</topic><topic>Carbohydrate Sequence</topic><topic>Glucuronidase - metabolism</topic><topic>Hydrolysis</topic><topic>Kinetics</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Polysaccharides - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TSUCHIHASHI, Hiroshi</creatorcontrib><creatorcontrib>YADOMAE, Toshiro</creatorcontrib><creatorcontrib>MIYAZAKI, Toshio</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biochemistry (Tokyo)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TSUCHIHASHI, Hiroshi</au><au>YADOMAE, Toshiro</au><au>MIYAZAKI, Toshio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protuberic Acid-Hydrolytic Enzyme in Kobayasia nipponica and Characterization of the Hydrolytic Products</atitle><jtitle>Journal of biochemistry (Tokyo)</jtitle><addtitle>J Biochem</addtitle><date>1983-10</date><risdate>1983</risdate><volume>94</volume><issue>4</issue><spage>1037</spage><epage>1044</epage><pages>1037-1044</pages><issn>0021-924X</issn><eissn>1756-2651</eissn><abstract>When the cold water-extract of Kobayasia nipponica containing a glycuronan, protuberic acid (PA), was allowed to stand at room temperature, PA was hydrolyzed. The optimum conditions for this PA-hydrolysis were 37°C and pH 4–5 in 0.1 M acetate buffer. Characterization of the hydrolytic products was performed by chemical analysis, and by 1H- and 13C-NMR spectroscopy. They were identified as D-GlcUA and O-(α-L-IdUAp)-(l-4)-D-GlcUA. These results suggest that PA-hydrolytic enzyme(s) include at least endo-β-D-glucuronidase.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>6654843</pmid><doi>10.1093/oxfordjournals.jbchem.a134445</doi><tpages>8</tpages></addata></record> |
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| ispartof | Journal of biochemistry (Tokyo), 1983-10, Vol.94 (4), p.1037-1044 |
| issn | 0021-924X 1756-2651 |
| language | eng |
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| source | J-STAGE Free; MEDLINE; Oxford University Press Archive; Free Full-Text Journals in Chemistry |
| subjects | Basidiomycota - enzymology Carbohydrate Sequence Glucuronidase - metabolism Hydrolysis Kinetics Magnetic Resonance Spectroscopy Polysaccharides - isolation & purification |
| title | Protuberic Acid-Hydrolytic Enzyme in Kobayasia nipponica and Characterization of the Hydrolytic Products |
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