Aggregation of porcine pancreatic phospholipase A2 and its zymogen induced by submicellar concentrations of negatively charged detergents

The interaction of two sodium n-alkyl sulfates (C10 and C12) with porcine pancreatic phospholipase A2 and its zymogen was studied by various spectroscopic techniques, equilibrium gel filtration, calorimetry, and photochemically induced dynamic nuclear polarization 1H NMR. At very low concentrations...

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Veröffentlicht in:	Biochemistry (Easton) 1983-11, Vol.22 (23), p.5347-5353
Hauptverfasser:	HILLE, J. D. R, EGMOND, M. R, DIJKMAN, R, VAN OORT, M. G, JIRGENSONS, B, DE HAAS, G. H
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Calorimetry Circular Dichroism Detergents - metabolism Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Hydrolases Micelles Molecular Weight Pancreas - enzymology Phospholipases - metabolism Phospholipases A - metabolism Phospholipases A2 Sulfuric Acid Esters - metabolism Surface-Active Agents - metabolism Swine
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