Scrapie infectivity, fibrils and low molecular weight protein
The development of a short incubation model of scrapie (strain 263K), in golden hamsters 1,2 , has added impetus to the purification of the infectious agent. Our own attempts 3 have been based on methods pioneered by Millson 4,5 and developed by Prusiner 6,7 . We present here results indicating that...
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| Veröffentlicht in: | Nature (London) 1983-12, Vol.306 (5942), p.476-478 |
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| container_issue | 5942 |
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| container_title | Nature (London) |
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| creator | Diringer, H Gelderblom, H Hilmert, H Ozel, M Edelbluth, C |
| description | The development of a short incubation model of scrapie (strain 263K), in golden hamsters
1,2
, has added impetus to the purification of the infectious agent. Our own attempts
3
have been based on methods pioneered by Millson
4,5
and developed by Prusiner
6,7
. We present here results indicating that a purification factor of up to 10
4
with respect to protein may now be possible. Fractions from brain with high infectivity had a sedimentation range of 70–300S and contained an abundance of fibrils closely similar to the scrapie-associated fibrils (SAF) discovered by Merz
et al.
8,9
. Material of molecular weight (
M
r
) 26,000, which is probably protein, appears to be a major constituent of the fibrils. The association between infectivity and fibrils raises two possibilities: the fibrils are an infectious form of the scrapie agent or they are a pathological response to scrapie infection. |
| doi_str_mv | 10.1038/306476a0 |
| format | Article |
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1,2
, has added impetus to the purification of the infectious agent. Our own attempts
3
have been based on methods pioneered by Millson
4,5
and developed by Prusiner
6,7
. We present here results indicating that a purification factor of up to 10
4
with respect to protein may now be possible. Fractions from brain with high infectivity had a sedimentation range of 70–300S and contained an abundance of fibrils closely similar to the scrapie-associated fibrils (SAF) discovered by Merz
et al.
8,9
. Material of molecular weight (
M
r
) 26,000, which is probably protein, appears to be a major constituent of the fibrils. The association between infectivity and fibrils raises two possibilities: the fibrils are an infectious form of the scrapie agent or they are a pathological response to scrapie infection.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/306476a0</identifier><identifier>PMID: 6685822</identifier><identifier>CODEN: NATUAS</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Animals ; Biological and medical sciences ; Brain - physiopathology ; Cricetinae ; Experimental viral diseases and models ; Humanities and Social Sciences ; Infectious diseases ; letter ; Medical sciences ; Molecular Weight ; multidisciplinary ; Proteins - isolation & purification ; purification ; Science ; Science (multidisciplinary) ; Scrapie - pathology ; Scrapie - transmission ; Sheep ; Viral diseases</subject><ispartof>Nature (London), 1983-12, Vol.306 (5942), p.476-478</ispartof><rights>Springer Nature Limited 1983</rights><rights>1984 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c462t-48a30351c50a7cbac5f44d303ba5f572a14b8a5da02e81fcff4bf39273f68ca73</citedby><cites>FETCH-LOGICAL-c462t-48a30351c50a7cbac5f44d303ba5f572a14b8a5da02e81fcff4bf39273f68ca73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/306476a0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/306476a0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9516504$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6685822$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Diringer, H</creatorcontrib><creatorcontrib>Gelderblom, H</creatorcontrib><creatorcontrib>Hilmert, H</creatorcontrib><creatorcontrib>Ozel, M</creatorcontrib><creatorcontrib>Edelbluth, C</creatorcontrib><title>Scrapie infectivity, fibrils and low molecular weight protein</title><title>Nature (London)</title><addtitle>Nature</addtitle><addtitle>Nature</addtitle><description>The development of a short incubation model of scrapie (strain 263K), in golden hamsters
1,2
, has added impetus to the purification of the infectious agent. Our own attempts
3
have been based on methods pioneered by Millson
4,5
and developed by Prusiner
6,7
. We present here results indicating that a purification factor of up to 10
4
with respect to protein may now be possible. Fractions from brain with high infectivity had a sedimentation range of 70–300S and contained an abundance of fibrils closely similar to the scrapie-associated fibrils (SAF) discovered by Merz
et al.
8,9
. Material of molecular weight (
M
r
) 26,000, which is probably protein, appears to be a major constituent of the fibrils. The association between infectivity and fibrils raises two possibilities: the fibrils are an infectious form of the scrapie agent or they are a pathological response to scrapie infection.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Brain - physiopathology</subject><subject>Cricetinae</subject><subject>Experimental viral diseases and models</subject><subject>Humanities and Social Sciences</subject><subject>Infectious diseases</subject><subject>letter</subject><subject>Medical sciences</subject><subject>Molecular Weight</subject><subject>multidisciplinary</subject><subject>Proteins - isolation & purification</subject><subject>purification</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Scrapie - pathology</subject><subject>Scrapie - transmission</subject><subject>Sheep</subject><subject>Viral diseases</subject><issn>0028-0836</issn><issn>1476-4687</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtLBDEQhIMouj7APyDOQUTB0c47e_Ag4gsED-p56Mkma2R2Zk1mFP-9kV09CZ666fqogmpCdimcUuDmjIMSWiGskBHNSymU0atkBMBMCYarDbKZ0isASKrFOllXykjD2IicP9qI8-CK0Hpn-_Ae-s-Twoc6hiYV2E6KpvsoZl3j7NBgLD5cmL70xTx2vQvtNlnz2CS3s5xb5Pn66unytrx_uLm7vLgvrVCsL4VBDlxSKwG1rdFKL8Qkn2qUXmqGVNQG5QSBOUO99V7Uno-Z5l4Zi5pvkcOFb859G1zqq1lI1jUNtq4bUmVAGyOE-Rek3ChNuczg0QK0sUspOl_NY5hh_KwoVN-VVj-VZnRv6TnUMzf5BZcdZv1gqWOy2PiIrQ3pFxtLqiSIjB0vsJSVdupi9doNsc21_RW5v2A9dhVOY7Z7fmRAOTA6zr9l_AvFn5Md</recordid><startdate>19831201</startdate><enddate>19831201</enddate><creator>Diringer, H</creator><creator>Gelderblom, H</creator><creator>Hilmert, H</creator><creator>Ozel, M</creator><creator>Edelbluth, C</creator><general>Nature Publishing Group UK</general><general>Nature Publishing</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19831201</creationdate><title>Scrapie infectivity, fibrils and low molecular weight protein</title><author>Diringer, H ; Gelderblom, H ; Hilmert, H ; Ozel, M ; Edelbluth, C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c462t-48a30351c50a7cbac5f44d303ba5f572a14b8a5da02e81fcff4bf39273f68ca73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Brain - physiopathology</topic><topic>Cricetinae</topic><topic>Experimental viral diseases and models</topic><topic>Humanities and Social Sciences</topic><topic>Infectious diseases</topic><topic>letter</topic><topic>Medical sciences</topic><topic>Molecular Weight</topic><topic>multidisciplinary</topic><topic>Proteins - isolation & purification</topic><topic>purification</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Scrapie - pathology</topic><topic>Scrapie - transmission</topic><topic>Sheep</topic><topic>Viral diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Diringer, H</creatorcontrib><creatorcontrib>Gelderblom, H</creatorcontrib><creatorcontrib>Hilmert, H</creatorcontrib><creatorcontrib>Ozel, M</creatorcontrib><creatorcontrib>Edelbluth, C</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Nature (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Diringer, H</au><au>Gelderblom, H</au><au>Hilmert, H</au><au>Ozel, M</au><au>Edelbluth, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Scrapie infectivity, fibrils and low molecular weight protein</atitle><jtitle>Nature (London)</jtitle><stitle>Nature</stitle><addtitle>Nature</addtitle><date>1983-12-01</date><risdate>1983</risdate><volume>306</volume><issue>5942</issue><spage>476</spage><epage>478</epage><pages>476-478</pages><issn>0028-0836</issn><eissn>1476-4687</eissn><coden>NATUAS</coden><abstract>The development of a short incubation model of scrapie (strain 263K), in golden hamsters
1,2
, has added impetus to the purification of the infectious agent. Our own attempts
3
have been based on methods pioneered by Millson
4,5
and developed by Prusiner
6,7
. We present here results indicating that a purification factor of up to 10
4
with respect to protein may now be possible. Fractions from brain with high infectivity had a sedimentation range of 70–300S and contained an abundance of fibrils closely similar to the scrapie-associated fibrils (SAF) discovered by Merz
et al.
8,9
. Material of molecular weight (
M
r
) 26,000, which is probably protein, appears to be a major constituent of the fibrils. The association between infectivity and fibrils raises two possibilities: the fibrils are an infectious form of the scrapie agent or they are a pathological response to scrapie infection.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>6685822</pmid><doi>10.1038/306476a0</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0028-0836 |
| ispartof | Nature (London), 1983-12, Vol.306 (5942), p.476-478 |
| issn | 0028-0836 1476-4687 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80788448 |
| source | MEDLINE; SpringerLink Journals; Nature |
| subjects | Animals Biological and medical sciences Brain - physiopathology Cricetinae Experimental viral diseases and models Humanities and Social Sciences Infectious diseases letter Medical sciences Molecular Weight multidisciplinary Proteins - isolation & purification purification Science Science (multidisciplinary) Scrapie - pathology Scrapie - transmission Sheep Viral diseases |
| title | Scrapie infectivity, fibrils and low molecular weight protein |
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