CALCIUM-EVOKED OPENING OF POTASSIUM CHANNELS IN HAMSTER EGGS
Measurements of membrane potential and resistance have been made in zona-free hamster eggs. The resting potential lay in the range -9 to -100 mV and the input resistance fell in the range 14 to 440 MΩ; high resting potentials were associated with large input resistances. Calcium injected ionophoret...
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| Veröffentlicht in: | Experimental physiology 1983-10, Vol.68 (4), p.687-700 |
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| container_title | Experimental physiology |
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| creator | Georgiou, P. Bountra, C. Bland, K. P. House, C. R. |
| description | Measurements of membrane potential and resistance have been made in zona-free hamster eggs. The resting potential lay in the
range -9 to -100 mV and the input resistance fell in the range 14 to 440 MΩ; high resting potentials were associated with
large input resistances. Calcium injected ionophoretically into an egg from an intracellular micro-electrode caused a reduction
of the membrane resistance. The estimated reversal potential for the calcium-evoked response was about -80 mV and its amplitude
depended on the extracellular concentration of potassium but not on the chloride concentration. We conclude that membrane
potassium channels open in response to a rise in the cytosolic concentration of calcium ions. Evidence is presented to suggest
that micro-electrode recordings of the membrane potential and resistance of eggs suffer from an impalement leak artifact.
The presence of the artifact lowers the resting potential and resistance of the cell so that intracellular calcium injection
causes a hyperpolarization. We conclude that a hyperpolarizing response to calcium would be unlikely in the absence of an
impalement artifact. |
| doi_str_mv | 10.1113/expphysiol.1983.sp002758 |
| format | Article |
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range -9 to -100 mV and the input resistance fell in the range 14 to 440 MΩ; high resting potentials were associated with
large input resistances. Calcium injected ionophoretically into an egg from an intracellular micro-electrode caused a reduction
of the membrane resistance. The estimated reversal potential for the calcium-evoked response was about -80 mV and its amplitude
depended on the extracellular concentration of potassium but not on the chloride concentration. We conclude that membrane
potassium channels open in response to a rise in the cytosolic concentration of calcium ions. Evidence is presented to suggest
that micro-electrode recordings of the membrane potential and resistance of eggs suffer from an impalement leak artifact.
The presence of the artifact lowers the resting potential and resistance of the cell so that intracellular calcium injection
causes a hyperpolarization. We conclude that a hyperpolarizing response to calcium would be unlikely in the absence of an
impalement artifact.</description><identifier>ISSN: 0958-0670</identifier><identifier>ISSN: 0144-8757</identifier><identifier>EISSN: 1469-445X</identifier><identifier>DOI: 10.1113/expphysiol.1983.sp002758</identifier><identifier>PMID: 6316399</identifier><identifier>CODEN: QJEPD3</identifier><language>eng</language><publisher>Cambridge: The Physiological Society</publisher><subject>Animals ; Biological and medical sciences ; Calcium - pharmacology ; Calcium - physiology ; Cricetinae - physiology ; Early stages. Segmentation. Gastrulation. Neurulation ; Electric Conductivity ; Embryology: invertebrates and vertebrates. Teratology ; Female ; Fundamental and applied biological sciences. Psychology ; Ion Channels - physiology ; Iontophoresis ; Membrane Potentials ; Ovum - drug effects ; Ovum - physiology ; Potassium - metabolism</subject><ispartof>Experimental physiology, 1983-10, Vol.68 (4), p.687-700</ispartof><rights>1983 The Physiological Society</rights><rights>1984 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4967-1efcd6705d3b64d602763da46c553e7a79fc7301e9d473e3b5175f57e97ba0273</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27907,27908</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9715775$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6316399$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Georgiou, P.</creatorcontrib><creatorcontrib>Bountra, C.</creatorcontrib><creatorcontrib>Bland, K. P.</creatorcontrib><creatorcontrib>House, C. R.</creatorcontrib><title>CALCIUM-EVOKED OPENING OF POTASSIUM CHANNELS IN HAMSTER EGGS</title><title>Experimental physiology</title><addtitle>Q J Exp Physiol</addtitle><description>Measurements of membrane potential and resistance have been made in zona-free hamster eggs. The resting potential lay in the
range -9 to -100 mV and the input resistance fell in the range 14 to 440 MΩ; high resting potentials were associated with
large input resistances. Calcium injected ionophoretically into an egg from an intracellular micro-electrode caused a reduction
of the membrane resistance. The estimated reversal potential for the calcium-evoked response was about -80 mV and its amplitude
depended on the extracellular concentration of potassium but not on the chloride concentration. We conclude that membrane
potassium channels open in response to a rise in the cytosolic concentration of calcium ions. Evidence is presented to suggest
that micro-electrode recordings of the membrane potential and resistance of eggs suffer from an impalement leak artifact.
The presence of the artifact lowers the resting potential and resistance of the cell so that intracellular calcium injection
causes a hyperpolarization. We conclude that a hyperpolarizing response to calcium would be unlikely in the absence of an
impalement artifact.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calcium - pharmacology</subject><subject>Calcium - physiology</subject><subject>Cricetinae - physiology</subject><subject>Early stages. Segmentation. Gastrulation. Neurulation</subject><subject>Electric Conductivity</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Ion Channels - physiology</subject><subject>Iontophoresis</subject><subject>Membrane Potentials</subject><subject>Ovum - drug effects</subject><subject>Ovum - physiology</subject><subject>Potassium - metabolism</subject><issn>0958-0670</issn><issn>0144-8757</issn><issn>1469-445X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMtOwzAURC0EKuXxCUhZIHYpNn5LbKoQ2oqSVKRF7CzXcWhQSkJMBf17XLU8lqwsec7cO3cACBDsIYTwpf1smsXalXXVQ1LgnmsgvOJU7IEuIkyGhNCnfdCFkooQMg4PwZFzLxAiDAXpgA7DiGEpu-A66o-j0ew-jB_Tu_gmSCdxMkoGQXobTNJpP8u8FkTDfpLE4ywYJcGwf59N44cgHgyyE3BQ6MrZ0917DGa38TQahuN0MPKDQ0Mk4yGyhcl9CJrjOSM580kZzjVhhlJsueayMBxDZGVOOLZ4ThGnBeVW8rn2MD4GF9u5TVu_rax7V8vSGVtV-tXWK6cE5IJiIj0otqBpa-daW6imLZe6XSsE1aY49Vuc2hSnvovz1rPdjtV8afMf464pr5_vdO2MropWv5rS_WCSI8o59Vi0xT7Kyq7_vV7Fk-HmgwnCxJ-DF-Xz4qNsrdq6XG1K--5DCUXUBvwCyQeWww</recordid><startdate>19831010</startdate><enddate>19831010</enddate><creator>Georgiou, P.</creator><creator>Bountra, C.</creator><creator>Bland, K. P.</creator><creator>House, C. R.</creator><general>The Physiological Society</general><general>Cambridge University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19831010</creationdate><title>CALCIUM-EVOKED OPENING OF POTASSIUM CHANNELS IN HAMSTER EGGS</title><author>Georgiou, P. ; Bountra, C. ; Bland, K. P. ; House, C. R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4967-1efcd6705d3b64d602763da46c553e7a79fc7301e9d473e3b5175f57e97ba0273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Calcium - pharmacology</topic><topic>Calcium - physiology</topic><topic>Cricetinae - physiology</topic><topic>Early stages. Segmentation. Gastrulation. Neurulation</topic><topic>Electric Conductivity</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Ion Channels - physiology</topic><topic>Iontophoresis</topic><topic>Membrane Potentials</topic><topic>Ovum - drug effects</topic><topic>Ovum - physiology</topic><topic>Potassium - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Georgiou, P.</creatorcontrib><creatorcontrib>Bountra, C.</creatorcontrib><creatorcontrib>Bland, K. P.</creatorcontrib><creatorcontrib>House, C. R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Georgiou, P.</au><au>Bountra, C.</au><au>Bland, K. P.</au><au>House, C. R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CALCIUM-EVOKED OPENING OF POTASSIUM CHANNELS IN HAMSTER EGGS</atitle><jtitle>Experimental physiology</jtitle><addtitle>Q J Exp Physiol</addtitle><date>1983-10-10</date><risdate>1983</risdate><volume>68</volume><issue>4</issue><spage>687</spage><epage>700</epage><pages>687-700</pages><issn>0958-0670</issn><issn>0144-8757</issn><eissn>1469-445X</eissn><coden>QJEPD3</coden><abstract>Measurements of membrane potential and resistance have been made in zona-free hamster eggs. The resting potential lay in the
range -9 to -100 mV and the input resistance fell in the range 14 to 440 MΩ; high resting potentials were associated with
large input resistances. Calcium injected ionophoretically into an egg from an intracellular micro-electrode caused a reduction
of the membrane resistance. The estimated reversal potential for the calcium-evoked response was about -80 mV and its amplitude
depended on the extracellular concentration of potassium but not on the chloride concentration. We conclude that membrane
potassium channels open in response to a rise in the cytosolic concentration of calcium ions. Evidence is presented to suggest
that micro-electrode recordings of the membrane potential and resistance of eggs suffer from an impalement leak artifact.
The presence of the artifact lowers the resting potential and resistance of the cell so that intracellular calcium injection
causes a hyperpolarization. We conclude that a hyperpolarizing response to calcium would be unlikely in the absence of an
impalement artifact.</abstract><cop>Cambridge</cop><pub>The Physiological Society</pub><pmid>6316399</pmid><doi>10.1113/expphysiol.1983.sp002758</doi><tpages>14</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0958-0670 |
| ispartof | Experimental physiology, 1983-10, Vol.68 (4), p.687-700 |
| issn | 0958-0670 0144-8757 1469-445X |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Biological and medical sciences Calcium - pharmacology Calcium - physiology Cricetinae - physiology Early stages. Segmentation. Gastrulation. Neurulation Electric Conductivity Embryology: invertebrates and vertebrates. Teratology Female Fundamental and applied biological sciences. Psychology Ion Channels - physiology Iontophoresis Membrane Potentials Ovum - drug effects Ovum - physiology Potassium - metabolism |
| title | CALCIUM-EVOKED OPENING OF POTASSIUM CHANNELS IN HAMSTER EGGS |
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