Nucleotide sequence of the partition locus of Escherichia coli plasmid pSC101
We report here the sequence of a 375-bp EcoRI- AvaI DNA fragment that previously has been shown to contain a locus (termed partition or par) responsible for stable maintenance of the pSC101 plasmid in growing cell populations. The DNA sequence of the par region encodes no obvious proteins and contai...
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| Veröffentlicht in: | Gene 1983-01, Vol.24 (2), p.309-315 |
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| container_title | Gene |
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| creator | Miller, Christine A. Tucker, William T. Meacock, Peter A. Gustaf sson, Petter Cohen, Stanley N. |
| description | We report here the sequence of a 375-bp
EcoRI-
AvaI DNA fragment that previously has been shown to contain a locus (termed partition or
par) responsible for stable maintenance of the pSC101 plasmid in growing cell populations. The DNA sequence of the
par region encodes no obvious proteins and contains no segments having the structural characteristics of transcriptional or translational start signals. However, segments of the par locus appear capable of forming regions of intra-strand secondary structure, one of which resembles a
rho-independent transcription terminator. Computer analysis shows regions within
par that have homology with sequences found near the origin of replication of the
Escherichia coli chromosome and of the pBR322 and ColEl plasmids. The
par sequence homology in the pBR322 and ColEl plasmids maps in the vicinity of sites that interact with the
E. coli replication factor Y and accomplishes initiation of DNA synthesis on single-strand templates. |
| doi_str_mv | 10.1016/0378-1119(83)90091-4 |
| format | Article |
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EcoRI-
AvaI DNA fragment that previously has been shown to contain a locus (termed partition or
par) responsible for stable maintenance of the pSC101 plasmid in growing cell populations. The DNA sequence of the
par region encodes no obvious proteins and contains no segments having the structural characteristics of transcriptional or translational start signals. However, segments of the par locus appear capable of forming regions of intra-strand secondary structure, one of which resembles a
rho-independent transcription terminator. Computer analysis shows regions within
par that have homology with sequences found near the origin of replication of the
Escherichia coli chromosome and of the pBR322 and ColEl plasmids. The
par sequence homology in the pBR322 and ColEl plasmids maps in the vicinity of sites that interact with the
E. coli replication factor Y and accomplishes initiation of DNA synthesis on single-strand templates.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(83)90091-4</identifier><identifier>PMID: 6357952</identifier><identifier>CODEN: GENED6</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Bacteriology ; Base Sequence ; Biological and medical sciences ; Biotechnology ; Chromosome Mapping ; DNA Replication ; DNA, Bacterial - genetics ; Escherichia coli ; Escherichia coli - genetics ; Fundamental and applied biological sciences. Psychology ; Genes, Bacterial ; Genetic engineering ; Genetic technics ; Genetics ; Methods. Procedures. Technologies ; Microbiology ; Plasmids ; Recombinant DNA ; replication ; segregation ; stability ; Synthetic digonucleotides and genes. Sequencing ; transcription terminator</subject><ispartof>Gene, 1983-01, Vol.24 (2), p.309-315</ispartof><rights>1983</rights><rights>1984 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-7d73238988973fe1e91e52e78a7b32e743dcb380bcec88bc573327e84dfa9c3</citedby><cites>FETCH-LOGICAL-c417t-7d73238988973fe1e91e52e78a7b32e743dcb380bcec88bc573327e84dfa9c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0378111983900914$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9390435$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6357952$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miller, Christine A.</creatorcontrib><creatorcontrib>Tucker, William T.</creatorcontrib><creatorcontrib>Meacock, Peter A.</creatorcontrib><creatorcontrib>Gustaf sson, Petter</creatorcontrib><creatorcontrib>Cohen, Stanley N.</creatorcontrib><title>Nucleotide sequence of the partition locus of Escherichia coli plasmid pSC101</title><title>Gene</title><addtitle>Gene</addtitle><description>We report here the sequence of a 375-bp
EcoRI-
AvaI DNA fragment that previously has been shown to contain a locus (termed partition or
par) responsible for stable maintenance of the pSC101 plasmid in growing cell populations. The DNA sequence of the
par region encodes no obvious proteins and contains no segments having the structural characteristics of transcriptional or translational start signals. However, segments of the par locus appear capable of forming regions of intra-strand secondary structure, one of which resembles a
rho-independent transcription terminator. Computer analysis shows regions within
par that have homology with sequences found near the origin of replication of the
Escherichia coli chromosome and of the pBR322 and ColEl plasmids. The
par sequence homology in the pBR322 and ColEl plasmids maps in the vicinity of sites that interact with the
E. coli replication factor Y and accomplishes initiation of DNA synthesis on single-strand templates.</description><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Chromosome Mapping</subject><subject>DNA Replication</subject><subject>DNA, Bacterial - genetics</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Genetics</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbiology</subject><subject>Plasmids</subject><subject>Recombinant DNA</subject><subject>replication</subject><subject>segregation</subject><subject>stability</subject><subject>Synthetic digonucleotides and genes. Sequencing</subject><subject>transcription terminator</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkVFr2zAQx8VoybJu36ADP5SyPbiVfHYkvRRKyNZC2j5k70I-n4mKE3mSXei3n7yEPG56OdD97vTnJ8YuBb8RXCxuOUiVCyH0NwXfNeda5OUHNhdK6pxzUGdsfkI-sk8xvvJ0qqqYsdkCKqmrYs6enkfsyA-uoSzS75H2SJlvs2FLWW_D4Abn91nncYzT9SriloLDrbMZ-s5lfWfjzjVZv1mmUJ_ZeWu7SF-O9YJtfqx-LR_y9cvPx-X9OsdSyCGXjYQClFZKS2hJkBZUFSSVlTWkWkKDNSheI6FSNVYSoJCkyqa1GuGCXR-29sGnxHEwOxeRus7uyY_RKC4XlV5U_wUFJG1SQQLLA4jBxxioNX1wOxvejeBmkm0mk2YyaRSYv7JNmca-HveP9Y6a09DRbupfHfs2ou3aYPfo4gnT6fkSpph3B4ySsjdHwUR000c0LhAOpvHu3zn-AFubmZg</recordid><startdate>19830101</startdate><enddate>19830101</enddate><creator>Miller, Christine A.</creator><creator>Tucker, William T.</creator><creator>Meacock, Peter A.</creator><creator>Gustaf sson, Petter</creator><creator>Cohen, Stanley N.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19830101</creationdate><title>Nucleotide sequence of the partition locus of Escherichia coli plasmid pSC101</title><author>Miller, Christine A. ; Tucker, William T. ; Meacock, Peter A. ; Gustaf sson, Petter ; Cohen, Stanley N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-7d73238988973fe1e91e52e78a7b32e743dcb380bcec88bc573327e84dfa9c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Chromosome Mapping</topic><topic>DNA Replication</topic><topic>DNA, Bacterial - genetics</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Bacterial</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Genetics</topic><topic>Methods. Procedures. Technologies</topic><topic>Microbiology</topic><topic>Plasmids</topic><topic>Recombinant DNA</topic><topic>replication</topic><topic>segregation</topic><topic>stability</topic><topic>Synthetic digonucleotides and genes. Sequencing</topic><topic>transcription terminator</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miller, Christine A.</creatorcontrib><creatorcontrib>Tucker, William T.</creatorcontrib><creatorcontrib>Meacock, Peter A.</creatorcontrib><creatorcontrib>Gustaf sson, Petter</creatorcontrib><creatorcontrib>Cohen, Stanley N.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miller, Christine A.</au><au>Tucker, William T.</au><au>Meacock, Peter A.</au><au>Gustaf sson, Petter</au><au>Cohen, Stanley N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nucleotide sequence of the partition locus of Escherichia coli plasmid pSC101</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1983-01-01</date><risdate>1983</risdate><volume>24</volume><issue>2</issue><spage>309</spage><epage>315</epage><pages>309-315</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><coden>GENED6</coden><abstract>We report here the sequence of a 375-bp
EcoRI-
AvaI DNA fragment that previously has been shown to contain a locus (termed partition or
par) responsible for stable maintenance of the pSC101 plasmid in growing cell populations. The DNA sequence of the
par region encodes no obvious proteins and contains no segments having the structural characteristics of transcriptional or translational start signals. However, segments of the par locus appear capable of forming regions of intra-strand secondary structure, one of which resembles a
rho-independent transcription terminator. Computer analysis shows regions within
par that have homology with sequences found near the origin of replication of the
Escherichia coli chromosome and of the pBR322 and ColEl plasmids. The
par sequence homology in the pBR322 and ColEl plasmids maps in the vicinity of sites that interact with the
E. coli replication factor Y and accomplishes initiation of DNA synthesis on single-strand templates.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>6357952</pmid><doi>10.1016/0378-1119(83)90091-4</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 1983-01, Vol.24 (2), p.309-315 |
| issn | 0378-1119 1879-0038 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Bacteriology Base Sequence Biological and medical sciences Biotechnology Chromosome Mapping DNA Replication DNA, Bacterial - genetics Escherichia coli Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genes, Bacterial Genetic engineering Genetic technics Genetics Methods. Procedures. Technologies Microbiology Plasmids Recombinant DNA replication segregation stability Synthetic digonucleotides and genes. Sequencing transcription terminator |
| title | Nucleotide sequence of the partition locus of Escherichia coli plasmid pSC101 |
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