31P-NMR analysis of sea urchin sperm activation : Reversible formation of high energy phosphate compounds by changes in intracellular pH

31P-NMR has been used to estimate the internal pH (pH i ) of sperm from the sea urchin Strongylocentrotus purpuratus. The values for pH i obtained from the chemical shift of inorganic phosphate agree well with those obtained from amine accumulation. At low pH i , when sperm are quiescent (immotile a...

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Veröffentlicht in:	Experimental cell research 1983-11, Vol.149 (1), p.289-294
Hauptverfasser:	Christen, R., Schackmann, R.W., Dahlquist, F.W., Shapiro, B.M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - metabolism Animals Biological and medical sciences Early stages. Segmentation. Gastrulation. Neurulation Embryology: invertebrates and vertebrates. Teratology Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Magnetic Resonance Spectroscopy Male Oxygen Consumption Phosphates - metabolism Phosphocreatine - metabolism Sea Urchins Sperm Motility Spermatozoa - metabolism Spermatozoa - physiology
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