Intranuclear distribution of satellite DNA from kangaroo rat
Nuclei, nucleoli and three chromatin fractions of varying density were prepared from the livers of kangaroo rats, which have an unusually large content of two satellite DNAs. These components were incubated with sodium dodecyl sulfate and Pronase and were submitted to analytical buoyant density ultr...
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| Veröffentlicht in: | Experimental cell research 1970-12, Vol.63 (2), p.462-466 |
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| container_title | Experimental cell research |
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| creator | Mazrimas, J.A. Hatch, F.T. |
| description | Nuclei, nucleoli and three chromatin fractions of varying density were prepared from the livers of kangaroo rats, which have an unusually large content of two satellite DNAs. These components were incubated with sodium dodecyl sulfate and Pronase and were submitted to analytical buoyant density ultracentrifugation in CsCl gradients. The nucleoli and heavy chromatin fractions were markedly enriched in the satellite DNAs. The distribution was similar to that previously reported in the laboratory mouse; however, in the kangaroo rat about 48% of the total cell DNA was present as satellites. |
| doi_str_mv | 10.1016/0014-4827(70)90240-5 |
| format | Article |
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These components were incubated with sodium dodecyl sulfate and Pronase and were submitted to analytical buoyant density ultracentrifugation in CsCl gradients. The nucleoli and heavy chromatin fractions were markedly enriched in the satellite DNAs. The distribution was similar to that previously reported in the laboratory mouse; however, in the kangaroo rat about 48% of the total cell DNA was present as satellites.</description><identifier>ISSN: 0014-4827</identifier><identifier>EISSN: 1090-2422</identifier><identifier>DOI: 10.1016/0014-4827(70)90240-5</identifier><identifier>PMID: 5490345</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cell Nucleolus - analysis ; Cell Nucleus - analysis ; Centrifugation, Density Gradient ; DNA - analysis ; Heterochromatin - analysis ; Liver - analysis ; Liver - cytology ; Methods ; Rodentia</subject><ispartof>Experimental cell research, 1970-12, Vol.63 (2), p.462-466</ispartof><rights>1970</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c272t-58854db4e8362793ebe8457481896d77d2b913c726a0107009d0257b83cb05f53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-4827(70)90240-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5490345$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mazrimas, J.A.</creatorcontrib><creatorcontrib>Hatch, F.T.</creatorcontrib><title>Intranuclear distribution of satellite DNA from kangaroo rat</title><title>Experimental cell research</title><addtitle>Exp Cell Res</addtitle><description>Nuclei, nucleoli and three chromatin fractions of varying density were prepared from the livers of kangaroo rats, which have an unusually large content of two satellite DNAs. These components were incubated with sodium dodecyl sulfate and Pronase and were submitted to analytical buoyant density ultracentrifugation in CsCl gradients. The nucleoli and heavy chromatin fractions were markedly enriched in the satellite DNAs. The distribution was similar to that previously reported in the laboratory mouse; however, in the kangaroo rat about 48% of the total cell DNA was present as satellites.</description><subject>Animals</subject><subject>Cell Nucleolus - analysis</subject><subject>Cell Nucleus - analysis</subject><subject>Centrifugation, Density Gradient</subject><subject>DNA - analysis</subject><subject>Heterochromatin - analysis</subject><subject>Liver - analysis</subject><subject>Liver - cytology</subject><subject>Methods</subject><subject>Rodentia</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1970</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9LxDAQxYMo67r6DRR6Ej1UJ2nSpCDCsv5bWPSi55CmqUTbZk1SwW9v6y579DSH9-bNvB9CpxiuMOD8GgDTlArCLzhcFkAopGwPTTEUkBJKyD6a7iyH6CiEDwAQAucTNGG0gIyyKbpZdtGrrteNUT6pbIjeln20rktcnQQVTdPYaJK753lSe9cmn6p7V965xKt4jA5q1QRzsp0z9PZw_7p4Slcvj8vFfJVqwklMmRCMViU1IssJLzJTGkEZpwKLIq84r0hZ4ExzkivAwAGKCgjjpch0Caxm2Qydb3LX3n31JkTZ2qCHz1RnXB-kAM4Y0GIw0o1RexeCN7Vce9sq_yMxyBGaHInIkYjkIP-gyTH_bJvfl62pdktbSoN-u9HNUPLbGi-DtqbTprLe6CgrZ_8_8AsF1HkM</recordid><startdate>197012</startdate><enddate>197012</enddate><creator>Mazrimas, J.A.</creator><creator>Hatch, F.T.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197012</creationdate><title>Intranuclear distribution of satellite DNA from kangaroo rat</title><author>Mazrimas, J.A. ; Hatch, F.T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c272t-58854db4e8362793ebe8457481896d77d2b913c726a0107009d0257b83cb05f53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1970</creationdate><topic>Animals</topic><topic>Cell Nucleolus - analysis</topic><topic>Cell Nucleus - analysis</topic><topic>Centrifugation, Density Gradient</topic><topic>DNA - analysis</topic><topic>Heterochromatin - analysis</topic><topic>Liver - analysis</topic><topic>Liver - cytology</topic><topic>Methods</topic><topic>Rodentia</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mazrimas, J.A.</creatorcontrib><creatorcontrib>Hatch, F.T.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mazrimas, J.A.</au><au>Hatch, F.T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intranuclear distribution of satellite DNA from kangaroo rat</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>1970-12</date><risdate>1970</risdate><volume>63</volume><issue>2</issue><spage>462</spage><epage>466</epage><pages>462-466</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><abstract>Nuclei, nucleoli and three chromatin fractions of varying density were prepared from the livers of kangaroo rats, which have an unusually large content of two satellite DNAs. These components were incubated with sodium dodecyl sulfate and Pronase and were submitted to analytical buoyant density ultracentrifugation in CsCl gradients. The nucleoli and heavy chromatin fractions were markedly enriched in the satellite DNAs. The distribution was similar to that previously reported in the laboratory mouse; however, in the kangaroo rat about 48% of the total cell DNA was present as satellites.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>5490345</pmid><doi>10.1016/0014-4827(70)90240-5</doi><tpages>5</tpages></addata></record> |
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| ispartof | Experimental cell research, 1970-12, Vol.63 (2), p.462-466 |
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| language | eng |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Animals Cell Nucleolus - analysis Cell Nucleus - analysis Centrifugation, Density Gradient DNA - analysis Heterochromatin - analysis Liver - analysis Liver - cytology Methods Rodentia |
| title | Intranuclear distribution of satellite DNA from kangaroo rat |
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