Vanadate affects glucose metabolism of human erythrocytes
Vanadate causes a rapid breakdown of 2,3-bisphosphoglycerate in intact erythrocytes. This metabolite is nearly stoichiometrically transformed into pyruvate, which changes the cell redox state and enhances the glycolytic flux. The results show that the vanadate effect on 2,3-bisphosphoglycerate, also...
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| Veröffentlicht in: | Archives of biochemistry and biophysics 1983-10, Vol.226 (2), p.441-447 |
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| container_issue | 2 |
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| container_title | Archives of biochemistry and biophysics |
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| creator | Ninfali, Paolino Accorsi, Augusto Fazi, Antonio Palma, Fulvio Fornaini, Giorgio |
| description | Vanadate causes a rapid breakdown of 2,3-bisphosphoglycerate in intact erythrocytes. This metabolite is nearly stoichiometrically transformed into pyruvate, which changes the cell redox state and enhances the glycolytic flux. The results show that the vanadate effect on 2,3-bisphosphoglycerate, also evident in hemolysates, is attributable to the stimulation of a phosphatase activity of the phosphoglycerate mutase. In agreement with others (
J. Carreras, F. Climent, R. Bartrons and G. Pons (1982)
Biochim. Biophys. Acta
705, 238–242
), vanadate is thought to destabilize the phosphoryl form of this enzyme which shows competitive inhibition between the ion and 2,3-bisphosphoglycerate in the mutase reaction. A competitive inhibition between vanadate and glucose 1,6-bisphosphate is also found for phosphoglucomutase, without evidence for phosphatase activity toward the bisphosphate cofactor. |
| doi_str_mv | 10.1016/0003-9861(83)90313-2 |
| format | Article |
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J. Carreras, F. Climent, R. Bartrons and G. Pons (1982)
Biochim. Biophys. Acta
705, 238–242
), vanadate is thought to destabilize the phosphoryl form of this enzyme which shows competitive inhibition between the ion and 2,3-bisphosphoglycerate in the mutase reaction. A competitive inhibition between vanadate and glucose 1,6-bisphosphate is also found for phosphoglucomutase, without evidence for phosphatase activity toward the bisphosphate cofactor.</description><identifier>ISSN: 0003-9861</identifier><identifier>EISSN: 1096-0384</identifier><identifier>DOI: 10.1016/0003-9861(83)90313-2</identifier><identifier>PMID: 6556902</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>2,3-Diphosphoglycerate ; Blood Glucose - metabolism ; Diphosphoglyceric Acids - blood ; Erythrocytes - drug effects ; Erythrocytes - metabolism ; Glycolysis - drug effects ; Humans ; Kinetics ; Vanadates ; Vanadium - pharmacology</subject><ispartof>Archives of biochemistry and biophysics, 1983-10, Vol.226 (2), p.441-447</ispartof><rights>1983</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-c4d5b4ab3ca033bb3a7a9133cc073d82404321ed58935ecde42ec4a6c77c7f573</citedby><cites>FETCH-LOGICAL-c357t-c4d5b4ab3ca033bb3a7a9133cc073d82404321ed58935ecde42ec4a6c77c7f573</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-9861(83)90313-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6556902$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ninfali, Paolino</creatorcontrib><creatorcontrib>Accorsi, Augusto</creatorcontrib><creatorcontrib>Fazi, Antonio</creatorcontrib><creatorcontrib>Palma, Fulvio</creatorcontrib><creatorcontrib>Fornaini, Giorgio</creatorcontrib><title>Vanadate affects glucose metabolism of human erythrocytes</title><title>Archives of biochemistry and biophysics</title><addtitle>Arch Biochem Biophys</addtitle><description>Vanadate causes a rapid breakdown of 2,3-bisphosphoglycerate in intact erythrocytes. This metabolite is nearly stoichiometrically transformed into pyruvate, which changes the cell redox state and enhances the glycolytic flux. The results show that the vanadate effect on 2,3-bisphosphoglycerate, also evident in hemolysates, is attributable to the stimulation of a phosphatase activity of the phosphoglycerate mutase. In agreement with others (
J. Carreras, F. Climent, R. Bartrons and G. Pons (1982)
Biochim. Biophys. Acta
705, 238–242
), vanadate is thought to destabilize the phosphoryl form of this enzyme which shows competitive inhibition between the ion and 2,3-bisphosphoglycerate in the mutase reaction. A competitive inhibition between vanadate and glucose 1,6-bisphosphate is also found for phosphoglucomutase, without evidence for phosphatase activity toward the bisphosphate cofactor.</description><subject>2,3-Diphosphoglycerate</subject><subject>Blood Glucose - metabolism</subject><subject>Diphosphoglyceric Acids - blood</subject><subject>Erythrocytes - drug effects</subject><subject>Erythrocytes - metabolism</subject><subject>Glycolysis - drug effects</subject><subject>Humans</subject><subject>Kinetics</subject><subject>Vanadates</subject><subject>Vanadium - pharmacology</subject><issn>0003-9861</issn><issn>1096-0384</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKxDAUhoMo4zj6BgpdiS6qSU_SNBtBBm8w4EbdhjQ5dSptMyatMG9vywwuXf2L_3I4HyHnjN4wyvJbSimkqsjZVQHXigKDNDsgc0ZVnlIo-CGZ_0WOyUmMX5QyxvNsRma5ELmi2ZyoD9MZZ3pMTFWh7WPy2QzWR0xa7E3pmzq2ia-S9dCaLsGw7dfB222P8ZQcVaaJeLbXBXl_fHhbPqer16eX5f0qtSBkn1ruRMlNCdZQgLIEI41iANZSCa7IOOWQMXSiUCDQOuQZWm5yK6WVlZCwIJe73U3w3wPGXrd1tNg0pkM_RF1QyZUSYgzyXdAGH2PASm9C3Zqw1YzqiZiecOgJhy5GnYjpbKxd7PeHskX3V9ojGv27nY_jkz81Bh1tjZ1FV4cRmHa-_v_AL3J4ejw</recordid><startdate>19831015</startdate><enddate>19831015</enddate><creator>Ninfali, Paolino</creator><creator>Accorsi, Augusto</creator><creator>Fazi, Antonio</creator><creator>Palma, Fulvio</creator><creator>Fornaini, Giorgio</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19831015</creationdate><title>Vanadate affects glucose metabolism of human erythrocytes</title><author>Ninfali, Paolino ; Accorsi, Augusto ; Fazi, Antonio ; Palma, Fulvio ; Fornaini, Giorgio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-c4d5b4ab3ca033bb3a7a9133cc073d82404321ed58935ecde42ec4a6c77c7f573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>2,3-Diphosphoglycerate</topic><topic>Blood Glucose - metabolism</topic><topic>Diphosphoglyceric Acids - blood</topic><topic>Erythrocytes - drug effects</topic><topic>Erythrocytes - metabolism</topic><topic>Glycolysis - drug effects</topic><topic>Humans</topic><topic>Kinetics</topic><topic>Vanadates</topic><topic>Vanadium - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ninfali, Paolino</creatorcontrib><creatorcontrib>Accorsi, Augusto</creatorcontrib><creatorcontrib>Fazi, Antonio</creatorcontrib><creatorcontrib>Palma, Fulvio</creatorcontrib><creatorcontrib>Fornaini, Giorgio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of biochemistry and biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ninfali, Paolino</au><au>Accorsi, Augusto</au><au>Fazi, Antonio</au><au>Palma, Fulvio</au><au>Fornaini, Giorgio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vanadate affects glucose metabolism of human erythrocytes</atitle><jtitle>Archives of biochemistry and biophysics</jtitle><addtitle>Arch Biochem Biophys</addtitle><date>1983-10-15</date><risdate>1983</risdate><volume>226</volume><issue>2</issue><spage>441</spage><epage>447</epage><pages>441-447</pages><issn>0003-9861</issn><eissn>1096-0384</eissn><abstract>Vanadate causes a rapid breakdown of 2,3-bisphosphoglycerate in intact erythrocytes. This metabolite is nearly stoichiometrically transformed into pyruvate, which changes the cell redox state and enhances the glycolytic flux. The results show that the vanadate effect on 2,3-bisphosphoglycerate, also evident in hemolysates, is attributable to the stimulation of a phosphatase activity of the phosphoglycerate mutase. In agreement with others (
J. Carreras, F. Climent, R. Bartrons and G. Pons (1982)
Biochim. Biophys. Acta
705, 238–242
), vanadate is thought to destabilize the phosphoryl form of this enzyme which shows competitive inhibition between the ion and 2,3-bisphosphoglycerate in the mutase reaction. A competitive inhibition between vanadate and glucose 1,6-bisphosphate is also found for phosphoglucomutase, without evidence for phosphatase activity toward the bisphosphate cofactor.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>6556902</pmid><doi>10.1016/0003-9861(83)90313-2</doi><tpages>7</tpages></addata></record> |
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| ispartof | Archives of biochemistry and biophysics, 1983-10, Vol.226 (2), p.441-447 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 2,3-Diphosphoglycerate Blood Glucose - metabolism Diphosphoglyceric Acids - blood Erythrocytes - drug effects Erythrocytes - metabolism Glycolysis - drug effects Humans Kinetics Vanadates Vanadium - pharmacology |
| title | Vanadate affects glucose metabolism of human erythrocytes |
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