Lack of endotoxin in Borrelia hispanica and Treponema pallidum
Borrelia hispanica from infected guinea pigs and Treponema pallidum from testicular syphilomas of rabbits were assayed for the presence of endotoxin with the Limulus lysate test. A suspension of Borrelia, containing 1.3 X 10(8) spirochetes/ml, was nonreactive both when it was tested as intact organi...
Gespeichert in:
| Veröffentlicht in: | Experimental biology and medicine (Maywood, N.J.) N.J.), 1983-10, Vol.174 (1), p.47-52 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 52 |
|---|---|
| container_issue | 1 |
| container_start_page | 47 |
| container_title | Experimental biology and medicine (Maywood, N.J.) |
| container_volume | 174 |
| creator | HARDY, P. H. JR LECIN, J |
| description | Borrelia hispanica from infected guinea pigs and Treponema pallidum from testicular syphilomas of rabbits were assayed for the presence of endotoxin with the Limulus lysate test. A suspension of Borrelia, containing 1.3 X 10(8) spirochetes/ml, was nonreactive both when it was tested as intact organisms, and when tested after disruption of the spirochetes by sonication. Eight different suspensions of treponemes, ranging from 0.6 X 10(9) to 3 X 10(9) treponemes/ml, were negative at a 1:10 dilution and were no more active than control suspensions of normal rabbit testes. Therefore, it was concluded that T. pallidum, as well as the Borrelia, possessed no endotoxin. |
| doi_str_mv | 10.3181/00379727-174-41702 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_80736313</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>13838883</sourcerecordid><originalsourceid>FETCH-LOGICAL-c358t-fecf2da14435774c2e0b12d02c14b1f6419ce303e757d13aa50c3014017a3dc93</originalsourceid><addsrcrecordid>eNqFkMtKxEAQRRtRxnH0BwQhC3EXrerqpJONoIMvGHAzrkNNp4PRvOyegP69iRPdCgW1uOfexRHiFOGSMMErANKpljpErUKFGuSemGMkoxBJ076Yj0A4EofiyPs3AIhBwkzMYopiJDUX1ys270FbBLbJ2237WTbBcLetc7YqOXgtfcdNaTjgJg_WznZtY2sOOq6qMu_rY3FQcOXtyfQX4uX-br18DFfPD0_Lm1VoKEq2YWFNIXNGpSjSWhlpYYMyB2lQbbCIFabGEpDVkc6RmCMwBKgANVNuUlqIi91u59qP3vptVpfe2Krixra9zxLQFBPSvyBSQkmSjKDcgca13jtbZJ0ra3ZfGUI22s1-7WaD3ezH7lA6m9b7TW3zv8qkc8jPp5y94apw3JjS_2FpLOVA0TcGjH-v</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>13838883</pqid></control><display><type>article</type><title>Lack of endotoxin in Borrelia hispanica and Treponema pallidum</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>HARDY, P. H. JR ; LECIN, J</creator><creatorcontrib>HARDY, P. H. JR ; LECIN, J</creatorcontrib><description>Borrelia hispanica from infected guinea pigs and Treponema pallidum from testicular syphilomas of rabbits were assayed for the presence of endotoxin with the Limulus lysate test. A suspension of Borrelia, containing 1.3 X 10(8) spirochetes/ml, was nonreactive both when it was tested as intact organisms, and when tested after disruption of the spirochetes by sonication. Eight different suspensions of treponemes, ranging from 0.6 X 10(9) to 3 X 10(9) treponemes/ml, were negative at a 1:10 dilution and were no more active than control suspensions of normal rabbit testes. Therefore, it was concluded that T. pallidum, as well as the Borrelia, possessed no endotoxin.</description><identifier>ISSN: 0037-9727</identifier><identifier>ISSN: 1535-3702</identifier><identifier>EISSN: 1525-1373</identifier><identifier>EISSN: 1535-3699</identifier><identifier>DOI: 10.3181/00379727-174-41702</identifier><identifier>PMID: 6356134</identifier><identifier>CODEN: PSEBAA</identifier><language>eng</language><publisher>Malden, MA: Blackwell Science</publisher><subject>Animals ; Bacteriology ; Biological and medical sciences ; Borrelia - analysis ; Endotoxins - analysis ; Fundamental and applied biological sciences. Psychology ; Guinea Pigs ; Limulus Test ; Male ; Microbiology ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; Rabbits ; Sonication ; Testis - analysis ; Treponema pallidum ; Treponema pallidum - analysis</subject><ispartof>Experimental biology and medicine (Maywood, N.J.), 1983-10, Vol.174 (1), p.47-52</ispartof><rights>1984 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c358t-fecf2da14435774c2e0b12d02c14b1f6419ce303e757d13aa50c3014017a3dc93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9622343$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6356134$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HARDY, P. H. JR</creatorcontrib><creatorcontrib>LECIN, J</creatorcontrib><title>Lack of endotoxin in Borrelia hispanica and Treponema pallidum</title><title>Experimental biology and medicine (Maywood, N.J.)</title><addtitle>Proc Soc Exp Biol Med</addtitle><description>Borrelia hispanica from infected guinea pigs and Treponema pallidum from testicular syphilomas of rabbits were assayed for the presence of endotoxin with the Limulus lysate test. A suspension of Borrelia, containing 1.3 X 10(8) spirochetes/ml, was nonreactive both when it was tested as intact organisms, and when tested after disruption of the spirochetes by sonication. Eight different suspensions of treponemes, ranging from 0.6 X 10(9) to 3 X 10(9) treponemes/ml, were negative at a 1:10 dilution and were no more active than control suspensions of normal rabbit testes. Therefore, it was concluded that T. pallidum, as well as the Borrelia, possessed no endotoxin.</description><subject>Animals</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Borrelia - analysis</subject><subject>Endotoxins - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Guinea Pigs</subject><subject>Limulus Test</subject><subject>Male</subject><subject>Microbiology</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>Rabbits</subject><subject>Sonication</subject><subject>Testis - analysis</subject><subject>Treponema pallidum</subject><subject>Treponema pallidum - analysis</subject><issn>0037-9727</issn><issn>1535-3702</issn><issn>1525-1373</issn><issn>1535-3699</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKxEAQRRtRxnH0BwQhC3EXrerqpJONoIMvGHAzrkNNp4PRvOyegP69iRPdCgW1uOfexRHiFOGSMMErANKpljpErUKFGuSemGMkoxBJ076Yj0A4EofiyPs3AIhBwkzMYopiJDUX1ys270FbBLbJ2237WTbBcLetc7YqOXgtfcdNaTjgJg_WznZtY2sOOq6qMu_rY3FQcOXtyfQX4uX-br18DFfPD0_Lm1VoKEq2YWFNIXNGpSjSWhlpYYMyB2lQbbCIFabGEpDVkc6RmCMwBKgANVNuUlqIi91u59qP3vptVpfe2Krixra9zxLQFBPSvyBSQkmSjKDcgca13jtbZJ0ra3ZfGUI22s1-7WaD3ezH7lA6m9b7TW3zv8qkc8jPp5y94apw3JjS_2FpLOVA0TcGjH-v</recordid><startdate>198310</startdate><enddate>198310</enddate><creator>HARDY, P. H. JR</creator><creator>LECIN, J</creator><general>Blackwell Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>198310</creationdate><title>Lack of endotoxin in Borrelia hispanica and Treponema pallidum</title><author>HARDY, P. H. JR ; LECIN, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c358t-fecf2da14435774c2e0b12d02c14b1f6419ce303e757d13aa50c3014017a3dc93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Animals</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Borrelia - analysis</topic><topic>Endotoxins - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Guinea Pigs</topic><topic>Limulus Test</topic><topic>Male</topic><topic>Microbiology</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>Rabbits</topic><topic>Sonication</topic><topic>Testis - analysis</topic><topic>Treponema pallidum</topic><topic>Treponema pallidum - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HARDY, P. H. JR</creatorcontrib><creatorcontrib>LECIN, J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HARDY, P. H. JR</au><au>LECIN, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lack of endotoxin in Borrelia hispanica and Treponema pallidum</atitle><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle><addtitle>Proc Soc Exp Biol Med</addtitle><date>1983-10</date><risdate>1983</risdate><volume>174</volume><issue>1</issue><spage>47</spage><epage>52</epage><pages>47-52</pages><issn>0037-9727</issn><issn>1535-3702</issn><eissn>1525-1373</eissn><eissn>1535-3699</eissn><coden>PSEBAA</coden><abstract>Borrelia hispanica from infected guinea pigs and Treponema pallidum from testicular syphilomas of rabbits were assayed for the presence of endotoxin with the Limulus lysate test. A suspension of Borrelia, containing 1.3 X 10(8) spirochetes/ml, was nonreactive both when it was tested as intact organisms, and when tested after disruption of the spirochetes by sonication. Eight different suspensions of treponemes, ranging from 0.6 X 10(9) to 3 X 10(9) treponemes/ml, were negative at a 1:10 dilution and were no more active than control suspensions of normal rabbit testes. Therefore, it was concluded that T. pallidum, as well as the Borrelia, possessed no endotoxin.</abstract><cop>Malden, MA</cop><pub>Blackwell Science</pub><pmid>6356134</pmid><doi>10.3181/00379727-174-41702</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0037-9727 |
| ispartof | Experimental biology and medicine (Maywood, N.J.), 1983-10, Vol.174 (1), p.47-52 |
| issn | 0037-9727 1535-3702 1525-1373 1535-3699 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80736313 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Bacteriology Biological and medical sciences Borrelia - analysis Endotoxins - analysis Fundamental and applied biological sciences. Psychology Guinea Pigs Limulus Test Male Microbiology Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Rabbits Sonication Testis - analysis Treponema pallidum Treponema pallidum - analysis |
| title | Lack of endotoxin in Borrelia hispanica and Treponema pallidum |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T01%3A20%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Lack%20of%20endotoxin%20in%20Borrelia%20hispanica%20and%20Treponema%20pallidum&rft.jtitle=Experimental%20biology%20and%20medicine%20(Maywood,%20N.J.)&rft.au=HARDY,%20P.%20H.%20JR&rft.date=1983-10&rft.volume=174&rft.issue=1&rft.spage=47&rft.epage=52&rft.pages=47-52&rft.issn=0037-9727&rft.eissn=1525-1373&rft.coden=PSEBAA&rft_id=info:doi/10.3181/00379727-174-41702&rft_dat=%3Cproquest_cross%3E13838883%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=13838883&rft_id=info:pmid/6356134&rfr_iscdi=true |