Autoradiographic differentiation of multiple benzodiazepine receptors by detergent solubilization and pharmacologic specificity
We have used light microscopic autoradiography to localize differentially Type 1 and 2 benzodiazepine receptors using pharmacologic and detergent treatment techniques. The triazolopyridazine CL218872 has preferential affinity for Type 1 receptors from which it displaces [ 3H]flunitrazepam selectivel...
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Veröffentlicht in: | Neuroscience letters 1983-08, Vol.39 (1), p.37-44 |
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creator | Lo, Mathew M.S. Niehoff, Debra L. Kuhar, Michael J. Snyder, Solomon H. |
description | We have used light microscopic autoradiography to localize differentially Type 1 and 2 benzodiazepine receptors using pharmacologic and detergent treatment techniques. The triazolopyridazine CL218872 has preferential affinity for Type 1 receptors from which it displaces [
3H]flunitrazepam selectively. In biochemical experiments [
3H]flunitrazepam binding sites with pharmacologic properties of Type 2 receptors are preferentially solubilized from brain membranes by detergents. We have now treated rat brain slices either with CL218872 or 2% sodium cholate and evaluated the autoradiographic distribution of [
3H]flunitrazepam-labeled receptors. Whether calculated from the drug or detergent treated preparations, the relative densities of apparent Type 1 and 2 receptors are the same. Thus differential sensitivity to detergent solubilization distinguishes the same two populations of receptors as Type 1 and 2 specific drugs. |
doi_str_mv | 10.1016/0304-3940(83)90162-3 |
format | Article |
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3H]flunitrazepam selectively. In biochemical experiments [
3H]flunitrazepam binding sites with pharmacologic properties of Type 2 receptors are preferentially solubilized from brain membranes by detergents. We have now treated rat brain slices either with CL218872 or 2% sodium cholate and evaluated the autoradiographic distribution of [
3H]flunitrazepam-labeled receptors. Whether calculated from the drug or detergent treated preparations, the relative densities of apparent Type 1 and 2 receptors are the same. Thus differential sensitivity to detergent solubilization distinguishes the same two populations of receptors as Type 1 and 2 specific drugs.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/0304-3940(83)90162-3</identifier><identifier>PMID: 6314190</identifier><identifier>CODEN: NELED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Animals ; Autoradiography ; benzodiazepines ; Binding, Competitive ; Biological and medical sciences ; brain ; Brain - metabolism ; Corpus Striatum - metabolism ; Flunitrazepam - metabolism ; Medical sciences ; multiple receptors ; Neural Pathways - metabolism ; Neuropharmacology ; Pharmacology. Drug treatments ; Psycholeptics: tranquillizer, neuroleptic ; Psychology. Psychoanalysis. Psychiatry ; Psychopharmacology ; Pyridazines - metabolism ; Rats ; Receptors, Cell Surface - metabolism ; Receptors, GABA-A ; Substantia Nigra - metabolism</subject><ispartof>Neuroscience letters, 1983-08, Vol.39 (1), p.37-44</ispartof><rights>1983</rights><rights>1984 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-fd870d27e93ad208a1fb506923c54a2b2a3f71a3c9aebd17ab43d2b74c4fda423</citedby><cites>FETCH-LOGICAL-c417t-fd870d27e93ad208a1fb506923c54a2b2a3f71a3c9aebd17ab43d2b74c4fda423</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0304394083901623$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9591149$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6314190$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lo, Mathew M.S.</creatorcontrib><creatorcontrib>Niehoff, Debra L.</creatorcontrib><creatorcontrib>Kuhar, Michael J.</creatorcontrib><creatorcontrib>Snyder, Solomon H.</creatorcontrib><title>Autoradiographic differentiation of multiple benzodiazepine receptors by detergent solubilization and pharmacologic specificity</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>We have used light microscopic autoradiography to localize differentially Type 1 and 2 benzodiazepine receptors using pharmacologic and detergent treatment techniques. The triazolopyridazine CL218872 has preferential affinity for Type 1 receptors from which it displaces [
3H]flunitrazepam selectively. In biochemical experiments [
3H]flunitrazepam binding sites with pharmacologic properties of Type 2 receptors are preferentially solubilized from brain membranes by detergents. We have now treated rat brain slices either with CL218872 or 2% sodium cholate and evaluated the autoradiographic distribution of [
3H]flunitrazepam-labeled receptors. Whether calculated from the drug or detergent treated preparations, the relative densities of apparent Type 1 and 2 receptors are the same. Thus differential sensitivity to detergent solubilization distinguishes the same two populations of receptors as Type 1 and 2 specific drugs.</description><subject>Animals</subject><subject>Autoradiography</subject><subject>benzodiazepines</subject><subject>Binding, Competitive</subject><subject>Biological and medical sciences</subject><subject>brain</subject><subject>Brain - metabolism</subject><subject>Corpus Striatum - metabolism</subject><subject>Flunitrazepam - metabolism</subject><subject>Medical sciences</subject><subject>multiple receptors</subject><subject>Neural Pathways - metabolism</subject><subject>Neuropharmacology</subject><subject>Pharmacology. Drug treatments</subject><subject>Psycholeptics: tranquillizer, neuroleptic</subject><subject>Psychology. Psychoanalysis. Psychiatry</subject><subject>Psychopharmacology</subject><subject>Pyridazines - metabolism</subject><subject>Rats</subject><subject>Receptors, Cell Surface - metabolism</subject><subject>Receptors, GABA-A</subject><subject>Substantia Nigra - metabolism</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU-LFDEQxYMo67j6DRT6IKKH1qSTnnQuwrL4Dxa86DlUJ5XZku5Om3QLMxe_uhlnmKOeClK_9yq8x9hzwd8KLrbvuOSqlkbx1518Y8pLU8sHbCM63dTa6OYh21yQx-xJzj84561o1RW72kqhhOEb9vtmXWICT3GXYL4nV3kKARNOC8FCcapiqMZ1WGgesOpxOkRPcMCZJqwSOpyLPFf9vvK4YNoVXZXjsPY00OFkAJOv5ntII7g4xF05kWd0FMjRsn_KHgUYMj47z2v2_eOHb7ef67uvn77c3tzVTgm91MF3mvtGo5HgG96BCH3Lt6aRrlXQ9A3IoAVIZwB7LzT0Svqm18qp4EE18pq9OvnOKf5cMS92pOxwGGDCuGbbcS1Fy_8PCrnVHW_bAqoT6FLMOWGwc6IR0t4Kbo8F2WP69pi-7aT9W5CVRfbi7L_2I_qL6NxI2b887yE7GEKCyVG-YKY1QihTsPcnDEtovwiTzY5wcuiptLJYH-nf__gDD7-wXg</recordid><startdate>19830819</startdate><enddate>19830819</enddate><creator>Lo, Mathew M.S.</creator><creator>Niehoff, Debra L.</creator><creator>Kuhar, Michael J.</creator><creator>Snyder, Solomon H.</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19830819</creationdate><title>Autoradiographic differentiation of multiple benzodiazepine receptors by detergent solubilization and pharmacologic specificity</title><author>Lo, Mathew M.S. ; Niehoff, Debra L. ; Kuhar, Michael J. ; Snyder, Solomon H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-fd870d27e93ad208a1fb506923c54a2b2a3f71a3c9aebd17ab43d2b74c4fda423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Animals</topic><topic>Autoradiography</topic><topic>benzodiazepines</topic><topic>Binding, Competitive</topic><topic>Biological and medical sciences</topic><topic>brain</topic><topic>Brain - metabolism</topic><topic>Corpus Striatum - metabolism</topic><topic>Flunitrazepam - metabolism</topic><topic>Medical sciences</topic><topic>multiple receptors</topic><topic>Neural Pathways - metabolism</topic><topic>Neuropharmacology</topic><topic>Pharmacology. Drug treatments</topic><topic>Psycholeptics: tranquillizer, neuroleptic</topic><topic>Psychology. Psychoanalysis. Psychiatry</topic><topic>Psychopharmacology</topic><topic>Pyridazines - metabolism</topic><topic>Rats</topic><topic>Receptors, Cell Surface - metabolism</topic><topic>Receptors, GABA-A</topic><topic>Substantia Nigra - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lo, Mathew M.S.</creatorcontrib><creatorcontrib>Niehoff, Debra L.</creatorcontrib><creatorcontrib>Kuhar, Michael J.</creatorcontrib><creatorcontrib>Snyder, Solomon H.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lo, Mathew M.S.</au><au>Niehoff, Debra L.</au><au>Kuhar, Michael J.</au><au>Snyder, Solomon H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Autoradiographic differentiation of multiple benzodiazepine receptors by detergent solubilization and pharmacologic specificity</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>1983-08-19</date><risdate>1983</risdate><volume>39</volume><issue>1</issue><spage>37</spage><epage>44</epage><pages>37-44</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>We have used light microscopic autoradiography to localize differentially Type 1 and 2 benzodiazepine receptors using pharmacologic and detergent treatment techniques. The triazolopyridazine CL218872 has preferential affinity for Type 1 receptors from which it displaces [
3H]flunitrazepam selectively. In biochemical experiments [
3H]flunitrazepam binding sites with pharmacologic properties of Type 2 receptors are preferentially solubilized from brain membranes by detergents. We have now treated rat brain slices either with CL218872 or 2% sodium cholate and evaluated the autoradiographic distribution of [
3H]flunitrazepam-labeled receptors. Whether calculated from the drug or detergent treated preparations, the relative densities of apparent Type 1 and 2 receptors are the same. Thus differential sensitivity to detergent solubilization distinguishes the same two populations of receptors as Type 1 and 2 specific drugs.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>6314190</pmid><doi>10.1016/0304-3940(83)90162-3</doi><tpages>8</tpages></addata></record> |
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subjects | Animals Autoradiography benzodiazepines Binding, Competitive Biological and medical sciences brain Brain - metabolism Corpus Striatum - metabolism Flunitrazepam - metabolism Medical sciences multiple receptors Neural Pathways - metabolism Neuropharmacology Pharmacology. Drug treatments Psycholeptics: tranquillizer, neuroleptic Psychology. Psychoanalysis. Psychiatry Psychopharmacology Pyridazines - metabolism Rats Receptors, Cell Surface - metabolism Receptors, GABA-A Substantia Nigra - metabolism |
title | Autoradiographic differentiation of multiple benzodiazepine receptors by detergent solubilization and pharmacologic specificity |
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