Comparison of bacterial communities in the alkaline gut segment among various species of higher termites
The first proctodeal (P1) segment in the hindgut of certain higher termites shows high alkalinity. We examined the bacterial diversity of the alkaline P1 gut segments of four species of higher termites by T-RFLP and phylogenetic analyses based on PCR-amplified 16S rRNA genes. The bacterial community...
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| Veröffentlicht in: | Extremophiles : life under extreme conditions 2005-06, Vol.9 (3), p.229-238 |
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| creator | THONGARAM, Taksawan HONGOH, Yuichi KOSONO, Saori OHKUMA, Moriya TRAKULNALEAMSAI, Savitr NOPARATNARAPORN, Napavarn KUDO, Toshiaki |
| description | The first proctodeal (P1) segment in the hindgut of certain higher termites shows high alkalinity. We examined the bacterial diversity of the alkaline P1 gut segments of four species of higher termites by T-RFLP and phylogenetic analyses based on PCR-amplified 16S rRNA genes. The bacterial community of the P1 segment was apparently different from that of the whole gut in each termite. Sequence analysis revealed that Firmicutes (Clostridia and Bacilli) were dominant in the P1 segments of all four termites; however, the phylogenetic compositions varied among the termites. Although some of the P1 segment-derived sequences were related to the sequences previously reported from the alkaline digestive tracts of other insects, most of them formed phylogenetic clusters unique to termites. Such "termite P1 clusters" were distantly related to known bacterial species as well as to sequences reported from alkaline environments in nature. We successfully obtained enrichment cultures of Clostridia- and Bacilli-related bacteria, including putative novel species under anaerobic alkaline conditions from the termite guts. Our results suggest that the alkaline gut region of termites harbors unique bacterial lineages and are expected to be a rich reservoir of novel alkaliphiles yet to be cultivated. |
| doi_str_mv | 10.1007/s00792-005-0440-9 |
| format | Article |
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We examined the bacterial diversity of the alkaline P1 gut segments of four species of higher termites by T-RFLP and phylogenetic analyses based on PCR-amplified 16S rRNA genes. The bacterial community of the P1 segment was apparently different from that of the whole gut in each termite. Sequence analysis revealed that Firmicutes (Clostridia and Bacilli) were dominant in the P1 segments of all four termites; however, the phylogenetic compositions varied among the termites. Although some of the P1 segment-derived sequences were related to the sequences previously reported from the alkaline digestive tracts of other insects, most of them formed phylogenetic clusters unique to termites. Such "termite P1 clusters" were distantly related to known bacterial species as well as to sequences reported from alkaline environments in nature. We successfully obtained enrichment cultures of Clostridia- and Bacilli-related bacteria, including putative novel species under anaerobic alkaline conditions from the termite guts. Our results suggest that the alkaline gut region of termites harbors unique bacterial lineages and are expected to be a rich reservoir of novel alkaliphiles yet to be cultivated.</description><identifier>ISSN: 1431-0651</identifier><identifier>EISSN: 1433-4909</identifier><identifier>DOI: 10.1007/s00792-005-0440-9</identifier><identifier>PMID: 15856134</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Alkalinity ; Animals ; Bacillus - classification ; Bacillus - genetics ; Bacillus - isolation & purification ; Bacteria ; Bacteria - classification ; Bacteria - isolation & purification ; Bacterial Physiological Phenomena ; Bacteriology ; Base Sequence ; Biological and medical sciences ; Clostridium - classification ; Clostridium - genetics ; Clostridium - isolation & purification ; Digestive System - microbiology ; DNA Primers ; Firmicutes ; Fundamental and applied biological sciences. Psychology ; Isoptera ; Isoptera - classification ; Isoptera - microbiology ; Microbiology ; Miscellaneous ; Phylogeny ; Polymorphism, Restriction Fragment Length ; RNA, Bacterial - genetics ; RNA, Ribosomal, 16S - genetics ; Space life sciences ; Termites</subject><ispartof>Extremophiles : life under extreme conditions, 2005-06, Vol.9 (3), p.229-238</ispartof><rights>2005 INIST-CNRS</rights><rights>Springer-Verlag 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c485t-10f9013865bdb47f42ec24af0b16c23dbf0be2150249f5ac0f5e09c40015f9fd3</citedby><cites>FETCH-LOGICAL-c485t-10f9013865bdb47f42ec24af0b16c23dbf0be2150249f5ac0f5e09c40015f9fd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16887882$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15856134$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>THONGARAM, Taksawan</creatorcontrib><creatorcontrib>HONGOH, Yuichi</creatorcontrib><creatorcontrib>KOSONO, Saori</creatorcontrib><creatorcontrib>OHKUMA, Moriya</creatorcontrib><creatorcontrib>TRAKULNALEAMSAI, Savitr</creatorcontrib><creatorcontrib>NOPARATNARAPORN, Napavarn</creatorcontrib><creatorcontrib>KUDO, Toshiaki</creatorcontrib><title>Comparison of bacterial communities in the alkaline gut segment among various species of higher termites</title><title>Extremophiles : life under extreme conditions</title><addtitle>Extremophiles</addtitle><description>The first proctodeal (P1) segment in the hindgut of certain higher termites shows high alkalinity. We examined the bacterial diversity of the alkaline P1 gut segments of four species of higher termites by T-RFLP and phylogenetic analyses based on PCR-amplified 16S rRNA genes. The bacterial community of the P1 segment was apparently different from that of the whole gut in each termite. Sequence analysis revealed that Firmicutes (Clostridia and Bacilli) were dominant in the P1 segments of all four termites; however, the phylogenetic compositions varied among the termites. Although some of the P1 segment-derived sequences were related to the sequences previously reported from the alkaline digestive tracts of other insects, most of them formed phylogenetic clusters unique to termites. Such "termite P1 clusters" were distantly related to known bacterial species as well as to sequences reported from alkaline environments in nature. We successfully obtained enrichment cultures of Clostridia- and Bacilli-related bacteria, including putative novel species under anaerobic alkaline conditions from the termite guts. Our results suggest that the alkaline gut region of termites harbors unique bacterial lineages and are expected to be a rich reservoir of novel alkaliphiles yet to be cultivated.</description><subject>Alkalinity</subject><subject>Animals</subject><subject>Bacillus - classification</subject><subject>Bacillus - genetics</subject><subject>Bacillus - isolation & purification</subject><subject>Bacteria</subject><subject>Bacteria - classification</subject><subject>Bacteria - isolation & purification</subject><subject>Bacterial Physiological Phenomena</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Clostridium - classification</subject><subject>Clostridium - genetics</subject><subject>Clostridium - isolation & purification</subject><subject>Digestive System - microbiology</subject><subject>DNA Primers</subject><subject>Firmicutes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Isoptera</subject><subject>Isoptera - classification</subject><subject>Isoptera - microbiology</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Phylogeny</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>RNA, Bacterial - genetics</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Space life sciences</subject><subject>Termites</subject><issn>1431-0651</issn><issn>1433-4909</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkU2LFDEQhoMo7jr6A7xIENRTa-Wzk-My-AULXvQc0plkJmsnGZNuwX9vxhlY8KCXqoI871spXoSeE3hLAMZ3rRdNBwAxAOcw6AfomnDGBq5BP_wzkwGkIFfoSWt3AET0h8foigglJGH8Gh22JR1tja1kXAKerFt8jXbGrqS05rhE33DMeDl4bOfvdo7Z4_264Ob3yecF21TyHv_sFmVtuB29Oym61SHuD77ibpfi4ttT9CjYuflnl75B3z68_7r9NNx--fh5e3M7OK7EMhAIGghTUky7iY-BU-8otwEmIh1lu6lPnhIBlOsgrIMgPGjHT7cFHXZsg96cfY-1_Fh9W0yKzfl5ttn3HxoFI9Ug-o4Nev1PUo6ajFSq_4JkBEkUkx18-Rd4V9aa-7lmFJoJPTLeIXKGXC2tVR_MscZk6y9DwJxiNedYTY_VnGI1umteXIzXKfndveKSYwdeXQDbnJ1DtdnFds9JpUalKPsNPNeqeQ</recordid><startdate>20050601</startdate><enddate>20050601</enddate><creator>THONGARAM, Taksawan</creator><creator>HONGOH, Yuichi</creator><creator>KOSONO, Saori</creator><creator>OHKUMA, Moriya</creator><creator>TRAKULNALEAMSAI, Savitr</creator><creator>NOPARATNARAPORN, Napavarn</creator><creator>KUDO, Toshiaki</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H95</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.G</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7SS</scope><scope>7T7</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20050601</creationdate><title>Comparison of bacterial communities in the alkaline gut segment among various species of higher termites</title><author>THONGARAM, Taksawan ; HONGOH, Yuichi ; KOSONO, Saori ; OHKUMA, Moriya ; TRAKULNALEAMSAI, Savitr ; NOPARATNARAPORN, Napavarn ; KUDO, Toshiaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c485t-10f9013865bdb47f42ec24af0b16c23dbf0be2150249f5ac0f5e09c40015f9fd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Alkalinity</topic><topic>Animals</topic><topic>Bacillus - classification</topic><topic>Bacillus - genetics</topic><topic>Bacillus - isolation & purification</topic><topic>Bacteria</topic><topic>Bacteria - classification</topic><topic>Bacteria - isolation & purification</topic><topic>Bacterial Physiological Phenomena</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Clostridium - classification</topic><topic>Clostridium - genetics</topic><topic>Clostridium - isolation & purification</topic><topic>Digestive System - microbiology</topic><topic>DNA Primers</topic><topic>Firmicutes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Isoptera</topic><topic>Isoptera - classification</topic><topic>Isoptera - microbiology</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Phylogeny</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>RNA, Bacterial - genetics</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Space life sciences</topic><topic>Termites</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>THONGARAM, Taksawan</creatorcontrib><creatorcontrib>HONGOH, Yuichi</creatorcontrib><creatorcontrib>KOSONO, Saori</creatorcontrib><creatorcontrib>OHKUMA, Moriya</creatorcontrib><creatorcontrib>TRAKULNALEAMSAI, Savitr</creatorcontrib><creatorcontrib>NOPARATNARAPORN, Napavarn</creatorcontrib><creatorcontrib>KUDO, Toshiaki</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest research library</collection><collection>ProQuest Science Journals</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Extremophiles : life under extreme conditions</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>THONGARAM, Taksawan</au><au>HONGOH, Yuichi</au><au>KOSONO, Saori</au><au>OHKUMA, Moriya</au><au>TRAKULNALEAMSAI, Savitr</au><au>NOPARATNARAPORN, Napavarn</au><au>KUDO, Toshiaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of bacterial communities in the alkaline gut segment among various species of higher termites</atitle><jtitle>Extremophiles : life under extreme conditions</jtitle><addtitle>Extremophiles</addtitle><date>2005-06-01</date><risdate>2005</risdate><volume>9</volume><issue>3</issue><spage>229</spage><epage>238</epage><pages>229-238</pages><issn>1431-0651</issn><eissn>1433-4909</eissn><abstract>The first proctodeal (P1) segment in the hindgut of certain higher termites shows high alkalinity. We examined the bacterial diversity of the alkaline P1 gut segments of four species of higher termites by T-RFLP and phylogenetic analyses based on PCR-amplified 16S rRNA genes. The bacterial community of the P1 segment was apparently different from that of the whole gut in each termite. Sequence analysis revealed that Firmicutes (Clostridia and Bacilli) were dominant in the P1 segments of all four termites; however, the phylogenetic compositions varied among the termites. Although some of the P1 segment-derived sequences were related to the sequences previously reported from the alkaline digestive tracts of other insects, most of them formed phylogenetic clusters unique to termites. Such "termite P1 clusters" were distantly related to known bacterial species as well as to sequences reported from alkaline environments in nature. We successfully obtained enrichment cultures of Clostridia- and Bacilli-related bacteria, including putative novel species under anaerobic alkaline conditions from the termite guts. Our results suggest that the alkaline gut region of termites harbors unique bacterial lineages and are expected to be a rich reservoir of novel alkaliphiles yet to be cultivated.</abstract><cop>Heidelberg</cop><cop>Tokyo</cop><pub>Springer</pub><pmid>15856134</pmid><doi>10.1007/s00792-005-0440-9</doi><tpages>10</tpages></addata></record> |
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| subjects | Alkalinity Animals Bacillus - classification Bacillus - genetics Bacillus - isolation & purification Bacteria Bacteria - classification Bacteria - isolation & purification Bacterial Physiological Phenomena Bacteriology Base Sequence Biological and medical sciences Clostridium - classification Clostridium - genetics Clostridium - isolation & purification Digestive System - microbiology DNA Primers Firmicutes Fundamental and applied biological sciences. Psychology Isoptera Isoptera - classification Isoptera - microbiology Microbiology Miscellaneous Phylogeny Polymorphism, Restriction Fragment Length RNA, Bacterial - genetics RNA, Ribosomal, 16S - genetics Space life sciences Termites |
| title | Comparison of bacterial communities in the alkaline gut segment among various species of higher termites |
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