Reduction of glycerol production to improve ethanol yield in an engineered Saccharomyces cerevisiae using glycerol as a substrate
Ethanol plays an important role in substituting the increasingly limited oil as the high-value, renewable fuel. In our previous studies, we successfully established the conversion of glycerol to ethanol by overexpression of p GcyaDak with p Gup1Cas in Saccharomyces cerevisiae. In addition to increas...
Gespeichert in:
| Veröffentlicht in: | Journal of biotechnology 2010-10, Vol.150 (2), p.209-214 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 214 |
|---|---|
| container_issue | 2 |
| container_start_page | 209 |
| container_title | Journal of biotechnology |
| container_volume | 150 |
| creator | Yu, Kyung Ok Kim, Seung Wook Han, Sung Ok |
| description | Ethanol plays an important role in substituting the increasingly limited oil as the high-value, renewable fuel. In our previous studies, we successfully established the conversion of glycerol to ethanol by overexpression of p
GcyaDak with p
Gup1Cas in
Saccharomyces cerevisiae. In addition to increasing ethanol production using glycerol as substrate, we minimized the synthesis of glycerol, which is the main by-product in ethanol fermentation processing. The glycerol production pathway was impaired by deletion of the genes
FPS1 and
GPD2. Strains deleted for both
FPS1 and
GPD2 reduce glycerol production and become highly sensitive to osmotic stress. We provide osmotic protection in YPH499
fps1Δgpd2Δ by overexpression of
Gup1. In this study,
S. cerevisiae using glycerol as substrate was modified through one-step gene disruption for redirection of glycerol carbon flux into ethanol by the deletion of two glycerol production genes,
FPS1 and
GPD2. The overall ethanol production in the modified strain YPH499
fps1Δgpd2Δ (p
GcyaDak, p
GupCas) was about 4.4
g
l
−1. These results demonstrate the possibility of providing protection against osmotic stress while simultaneously increasing ethanol and reducing glycerol production in
S. cerevisiae strains using glycerol as a carbon source. |
| doi_str_mv | 10.1016/j.jbiotec.2010.09.932 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_807290211</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0168165610018419</els_id><sourcerecordid>759878363</sourcerecordid><originalsourceid>FETCH-LOGICAL-c450t-42835747079e435e9bebed44770a1ed47c28a651c9e49543a7f71fbbf36825b73</originalsourceid><addsrcrecordid>eNqFkU1v1DAQhi0EotvCTwB8QZyy-DO2T6iqoCBVQqL0bDnOZOtVYhc7WWmP_HO82i099uTxzDPv2PMi9I6SNSW0_bxdb7uQZvBrRmqOmLXh7AVaUa14I3TLX6JV5XRDW9meofNStoQQYSR9jc4Y0VJoyVbo7y_oFz-HFHEa8Gbce8hpxA85PabnhMNU7zvAMN-7WKv7AGOPQ8QuYoibEAEy9PjWeX_vcpqqSMFVCHahBAd4KSFunsRdwQ6XpStzdjO8Qa8GNxZ4ezov0N23r7-vvjc3P69_XF3eNF5IMjeCaS6VUEQZEFyC6aCDXgiliKM1UJ5p10rqa9lIwZ0aFB26buCtZrJT_AJ9OurWv_xZoMx2CsXDOLoIaSlWE8UMYZQ-SypptNK85ZWUR9LnVEqGwT7kMLm8t5TYg012a0822YNNlhhbbap9708Tlm6C_n_Xoy8V-HgCXPFuHLKLPpQnjnNFNTlwH47c4JJ1m1yZu9s6iRNqiGDSVOLLkYC6212AbIsPED30IYOfbZ_CM4_9B6dkvjY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>759878363</pqid></control><display><type>article</type><title>Reduction of glycerol production to improve ethanol yield in an engineered Saccharomyces cerevisiae using glycerol as a substrate</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Yu, Kyung Ok ; Kim, Seung Wook ; Han, Sung Ok</creator><creatorcontrib>Yu, Kyung Ok ; Kim, Seung Wook ; Han, Sung Ok</creatorcontrib><description>Ethanol plays an important role in substituting the increasingly limited oil as the high-value, renewable fuel. In our previous studies, we successfully established the conversion of glycerol to ethanol by overexpression of p
GcyaDak with p
Gup1Cas in
Saccharomyces cerevisiae. In addition to increasing ethanol production using glycerol as substrate, we minimized the synthesis of glycerol, which is the main by-product in ethanol fermentation processing. The glycerol production pathway was impaired by deletion of the genes
FPS1 and
GPD2. Strains deleted for both
FPS1 and
GPD2 reduce glycerol production and become highly sensitive to osmotic stress. We provide osmotic protection in YPH499
fps1Δgpd2Δ by overexpression of
Gup1. In this study,
S. cerevisiae using glycerol as substrate was modified through one-step gene disruption for redirection of glycerol carbon flux into ethanol by the deletion of two glycerol production genes,
FPS1 and
GPD2. The overall ethanol production in the modified strain YPH499
fps1Δgpd2Δ (p
GcyaDak, p
GupCas) was about 4.4
g
l
−1. These results demonstrate the possibility of providing protection against osmotic stress while simultaneously increasing ethanol and reducing glycerol production in
S. cerevisiae strains using glycerol as a carbon source.</description><identifier>ISSN: 0168-1656</identifier><identifier>EISSN: 1873-4863</identifier><identifier>DOI: 10.1016/j.jbiotec.2010.09.932</identifier><identifier>PMID: 20854852</identifier><identifier>CODEN: JBITD4</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Biological and medical sciences ; Bioreactors - microbiology ; Biotechnology ; Ethanol ; Ethanol - metabolism ; Fermentation ; Fermentation - genetics ; Fundamental and applied biological sciences. Psychology ; Genetic Engineering - methods ; Glycerol ; Glycerol - metabolism ; Glycerol-3-Phosphate Dehydrogenase (NAD+) - genetics ; Membrane Proteins - genetics ; Membrane Transport Proteins - genetics ; Methods. Procedures. Technologies ; Microbial engineering. Fermentation and microbial culture technology ; Mutation ; Osmotic Pressure ; Phenotype ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae Proteins - genetics</subject><ispartof>Journal of biotechnology, 2010-10, Vol.150 (2), p.209-214</ispartof><rights>2010 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c450t-42835747079e435e9bebed44770a1ed47c28a651c9e49543a7f71fbbf36825b73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0168165610018419$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23371802$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20854852$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yu, Kyung Ok</creatorcontrib><creatorcontrib>Kim, Seung Wook</creatorcontrib><creatorcontrib>Han, Sung Ok</creatorcontrib><title>Reduction of glycerol production to improve ethanol yield in an engineered Saccharomyces cerevisiae using glycerol as a substrate</title><title>Journal of biotechnology</title><addtitle>J Biotechnol</addtitle><description>Ethanol plays an important role in substituting the increasingly limited oil as the high-value, renewable fuel. In our previous studies, we successfully established the conversion of glycerol to ethanol by overexpression of p
GcyaDak with p
Gup1Cas in
Saccharomyces cerevisiae. In addition to increasing ethanol production using glycerol as substrate, we minimized the synthesis of glycerol, which is the main by-product in ethanol fermentation processing. The glycerol production pathway was impaired by deletion of the genes
FPS1 and
GPD2. Strains deleted for both
FPS1 and
GPD2 reduce glycerol production and become highly sensitive to osmotic stress. We provide osmotic protection in YPH499
fps1Δgpd2Δ by overexpression of
Gup1. In this study,
S. cerevisiae using glycerol as substrate was modified through one-step gene disruption for redirection of glycerol carbon flux into ethanol by the deletion of two glycerol production genes,
FPS1 and
GPD2. The overall ethanol production in the modified strain YPH499
fps1Δgpd2Δ (p
GcyaDak, p
GupCas) was about 4.4
g
l
−1. These results demonstrate the possibility of providing protection against osmotic stress while simultaneously increasing ethanol and reducing glycerol production in
S. cerevisiae strains using glycerol as a carbon source.</description><subject>Biological and medical sciences</subject><subject>Bioreactors - microbiology</subject><subject>Biotechnology</subject><subject>Ethanol</subject><subject>Ethanol - metabolism</subject><subject>Fermentation</subject><subject>Fermentation - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Engineering - methods</subject><subject>Glycerol</subject><subject>Glycerol - metabolism</subject><subject>Glycerol-3-Phosphate Dehydrogenase (NAD+) - genetics</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Transport Proteins - genetics</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbial engineering. Fermentation and microbial culture technology</subject><subject>Mutation</subject><subject>Osmotic Pressure</subject><subject>Phenotype</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Saccharomyces cerevisiae Proteins - genetics</subject><issn>0168-1656</issn><issn>1873-4863</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EotvCTwB8QZyy-DO2T6iqoCBVQqL0bDnOZOtVYhc7WWmP_HO82i099uTxzDPv2PMi9I6SNSW0_bxdb7uQZvBrRmqOmLXh7AVaUa14I3TLX6JV5XRDW9meofNStoQQYSR9jc4Y0VJoyVbo7y_oFz-HFHEa8Gbce8hpxA85PabnhMNU7zvAMN-7WKv7AGOPQ8QuYoibEAEy9PjWeX_vcpqqSMFVCHahBAd4KSFunsRdwQ6XpStzdjO8Qa8GNxZ4ezov0N23r7-vvjc3P69_XF3eNF5IMjeCaS6VUEQZEFyC6aCDXgiliKM1UJ5p10rqa9lIwZ0aFB26buCtZrJT_AJ9OurWv_xZoMx2CsXDOLoIaSlWE8UMYZQ-SypptNK85ZWUR9LnVEqGwT7kMLm8t5TYg012a0822YNNlhhbbap9708Tlm6C_n_Xoy8V-HgCXPFuHLKLPpQnjnNFNTlwH47c4JJ1m1yZu9s6iRNqiGDSVOLLkYC6212AbIsPED30IYOfbZ_CM4_9B6dkvjY</recordid><startdate>20101015</startdate><enddate>20101015</enddate><creator>Yu, Kyung Ok</creator><creator>Kim, Seung Wook</creator><creator>Han, Sung Ok</creator><general>Elsevier B.V</general><general>[New York, NY]: Elsevier</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>20101015</creationdate><title>Reduction of glycerol production to improve ethanol yield in an engineered Saccharomyces cerevisiae using glycerol as a substrate</title><author>Yu, Kyung Ok ; Kim, Seung Wook ; Han, Sung Ok</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c450t-42835747079e435e9bebed44770a1ed47c28a651c9e49543a7f71fbbf36825b73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Biological and medical sciences</topic><topic>Bioreactors - microbiology</topic><topic>Biotechnology</topic><topic>Ethanol</topic><topic>Ethanol - metabolism</topic><topic>Fermentation</topic><topic>Fermentation - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Engineering - methods</topic><topic>Glycerol</topic><topic>Glycerol - metabolism</topic><topic>Glycerol-3-Phosphate Dehydrogenase (NAD+) - genetics</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Transport Proteins - genetics</topic><topic>Methods. Procedures. Technologies</topic><topic>Microbial engineering. Fermentation and microbial culture technology</topic><topic>Mutation</topic><topic>Osmotic Pressure</topic><topic>Phenotype</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Saccharomyces cerevisiae Proteins - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yu, Kyung Ok</creatorcontrib><creatorcontrib>Kim, Seung Wook</creatorcontrib><creatorcontrib>Han, Sung Ok</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yu, Kyung Ok</au><au>Kim, Seung Wook</au><au>Han, Sung Ok</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reduction of glycerol production to improve ethanol yield in an engineered Saccharomyces cerevisiae using glycerol as a substrate</atitle><jtitle>Journal of biotechnology</jtitle><addtitle>J Biotechnol</addtitle><date>2010-10-15</date><risdate>2010</risdate><volume>150</volume><issue>2</issue><spage>209</spage><epage>214</epage><pages>209-214</pages><issn>0168-1656</issn><eissn>1873-4863</eissn><coden>JBITD4</coden><abstract>Ethanol plays an important role in substituting the increasingly limited oil as the high-value, renewable fuel. In our previous studies, we successfully established the conversion of glycerol to ethanol by overexpression of p
GcyaDak with p
Gup1Cas in
Saccharomyces cerevisiae. In addition to increasing ethanol production using glycerol as substrate, we minimized the synthesis of glycerol, which is the main by-product in ethanol fermentation processing. The glycerol production pathway was impaired by deletion of the genes
FPS1 and
GPD2. Strains deleted for both
FPS1 and
GPD2 reduce glycerol production and become highly sensitive to osmotic stress. We provide osmotic protection in YPH499
fps1Δgpd2Δ by overexpression of
Gup1. In this study,
S. cerevisiae using glycerol as substrate was modified through one-step gene disruption for redirection of glycerol carbon flux into ethanol by the deletion of two glycerol production genes,
FPS1 and
GPD2. The overall ethanol production in the modified strain YPH499
fps1Δgpd2Δ (p
GcyaDak, p
GupCas) was about 4.4
g
l
−1. These results demonstrate the possibility of providing protection against osmotic stress while simultaneously increasing ethanol and reducing glycerol production in
S. cerevisiae strains using glycerol as a carbon source.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>20854852</pmid><doi>10.1016/j.jbiotec.2010.09.932</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-1656 |
| ispartof | Journal of biotechnology, 2010-10, Vol.150 (2), p.209-214 |
| issn | 0168-1656 1873-4863 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_807290211 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Biological and medical sciences Bioreactors - microbiology Biotechnology Ethanol Ethanol - metabolism Fermentation Fermentation - genetics Fundamental and applied biological sciences. Psychology Genetic Engineering - methods Glycerol Glycerol - metabolism Glycerol-3-Phosphate Dehydrogenase (NAD+) - genetics Membrane Proteins - genetics Membrane Transport Proteins - genetics Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology Mutation Osmotic Pressure Phenotype Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - genetics |
| title | Reduction of glycerol production to improve ethanol yield in an engineered Saccharomyces cerevisiae using glycerol as a substrate |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-26T14%3A32%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Reduction%20of%20glycerol%20production%20to%20improve%20ethanol%20yield%20in%20an%20engineered%20Saccharomyces%20cerevisiae%20using%20glycerol%20as%20a%20substrate&rft.jtitle=Journal%20of%20biotechnology&rft.au=Yu,%20Kyung%20Ok&rft.date=2010-10-15&rft.volume=150&rft.issue=2&rft.spage=209&rft.epage=214&rft.pages=209-214&rft.issn=0168-1656&rft.eissn=1873-4863&rft.coden=JBITD4&rft_id=info:doi/10.1016/j.jbiotec.2010.09.932&rft_dat=%3Cproquest_cross%3E759878363%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=759878363&rft_id=info:pmid/20854852&rft_els_id=S0168165610018419&rfr_iscdi=true |