Gene cloning and expression of aminopeptidase N and cadherin from midgut of the rice stem borer, Chilo suppressalis
The rice stem borer, Chilo suppressalis Walker is one of the most important insect pests on rice in Asia, north Africa and southern Europe. Transgenic Bt rice has been developed in the laboratory with good resistance to this pest and other Lepidopteran insects, which will provide a possible alternat...
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| Veröffentlicht in: | Insect science 2010-10, Vol.17 (5), p.393-399 |
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| creator | Yu, Hong-Kun Chen, Hao Zhang, Yong-Jun Wu, Kong-Ming Liang, Ge-Mei Liu, Ze-Wen Guo, Yu-Yuan |
| description | The rice stem borer, Chilo suppressalis Walker is one of the most important insect pests on rice in Asia, north Africa and southern Europe. Transgenic Bt rice has been developed in the laboratory with good resistance to this pest and other Lepidopteran insects, which will provide a possible alternative tool for this pest control. The full-length cDNAs encoding an aminopeptidase N （CsAPN） and a cadherin （CsCad） were cloned from C. suppressalis. CsAPN showed common features of, and high identities to, other insect APNs in its deduced amino acid sequence. Although a full-length cDNA encoding cadherin-like protein has been reported in GenBank, the newly isolated cadherin here （CsCad） showed some differences in its amino acid sequence, especially at the 7th cadherin repeat region （CR7）, which indicated the newly isolated CsCad might be another allele. CsAPN and CsCad were successfully expressed in insect Tn cells, and the blot analysis showed these two proteins could bind Bt toxin CrylAb. The results will provide valuable information for the studies of toxin mode of action and the possible toxin resistance mechanisms in this pest. |
| doi_str_mv | 10.1111/j.1744-7917.2010.01333.x |
| format | Article |
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Transgenic Bt rice has been developed in the laboratory with good resistance to this pest and other Lepidopteran insects, which will provide a possible alternative tool for this pest control. The full-length cDNAs encoding an aminopeptidase N （CsAPN） and a cadherin （CsCad） were cloned from C. suppressalis. CsAPN showed common features of, and high identities to, other insect APNs in its deduced amino acid sequence. Although a full-length cDNA encoding cadherin-like protein has been reported in GenBank, the newly isolated cadherin here （CsCad） showed some differences in its amino acid sequence, especially at the 7th cadherin repeat region （CR7）, which indicated the newly isolated CsCad might be another allele. CsAPN and CsCad were successfully expressed in insect Tn cells, and the blot analysis showed these two proteins could bind Bt toxin CrylAb. The results will provide valuable information for the studies of toxin mode of action and the possible toxin resistance mechanisms in this pest.</description><identifier>ISSN: 1672-9609</identifier><identifier>EISSN: 1744-7917</identifier><identifier>DOI: 10.1111/j.1744-7917.2010.01333.x</identifier><language>eng</language><publisher>Melbourne, Australia: Blackwell Publishing Asia</publisher><subject>Amino acid sequence ; Aminopeptidase ; aminopeptidase N ; Borers ; Cadherin ; Chilo suppressalis ; CsCad ; heterologous expression ; Lepidoptera ; membrane alanyl aminopeptidase ; Midgut ; Oryza sativa ; Pest control ; Pests ; Tn cells ; Toxins ; 二化螟 ; 氨肽酶N ; 水稻螟虫 ; 转Bt基因水稻 ; 钙粘蛋白 ; 鳞翅目昆虫</subject><ispartof>Insect science, 2010-10, Vol.17 (5), p.393-399</ispartof><rights>2010 The Authors Journal compilation © Institute of Zoology, Chinese Academy of Sciences</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4843-7d1709002800a179e107c977037ce793e73630c94d9917370b329b211b0c6fe03</citedby><cites>FETCH-LOGICAL-c4843-7d1709002800a179e107c977037ce793e73630c94d9917370b329b211b0c6fe03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84222A/84222A.jpg</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1744-7917.2010.01333.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1744-7917.2010.01333.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27929,27930,45579,45580</link.rule.ids></links><search><creatorcontrib>Yu, Hong-Kun</creatorcontrib><creatorcontrib>Chen, Hao</creatorcontrib><creatorcontrib>Zhang, Yong-Jun</creatorcontrib><creatorcontrib>Wu, Kong-Ming</creatorcontrib><creatorcontrib>Liang, Ge-Mei</creatorcontrib><creatorcontrib>Liu, Ze-Wen</creatorcontrib><creatorcontrib>Guo, Yu-Yuan</creatorcontrib><title>Gene cloning and expression of aminopeptidase N and cadherin from midgut of the rice stem borer, Chilo suppressalis</title><title>Insect science</title><addtitle>Insect Science</addtitle><description>The rice stem borer, Chilo suppressalis Walker is one of the most important insect pests on rice in Asia, north Africa and southern Europe. Transgenic Bt rice has been developed in the laboratory with good resistance to this pest and other Lepidopteran insects, which will provide a possible alternative tool for this pest control. The full-length cDNAs encoding an aminopeptidase N （CsAPN） and a cadherin （CsCad） were cloned from C. suppressalis. CsAPN showed common features of, and high identities to, other insect APNs in its deduced amino acid sequence. Although a full-length cDNA encoding cadherin-like protein has been reported in GenBank, the newly isolated cadherin here （CsCad） showed some differences in its amino acid sequence, especially at the 7th cadherin repeat region （CR7）, which indicated the newly isolated CsCad might be another allele. CsAPN and CsCad were successfully expressed in insect Tn cells, and the blot analysis showed these two proteins could bind Bt toxin CrylAb. The results will provide valuable information for the studies of toxin mode of action and the possible toxin resistance mechanisms in this pest.</description><subject>Amino acid sequence</subject><subject>Aminopeptidase</subject><subject>aminopeptidase N</subject><subject>Borers</subject><subject>Cadherin</subject><subject>Chilo suppressalis</subject><subject>CsCad</subject><subject>heterologous expression</subject><subject>Lepidoptera</subject><subject>membrane alanyl aminopeptidase</subject><subject>Midgut</subject><subject>Oryza sativa</subject><subject>Pest control</subject><subject>Pests</subject><subject>Tn cells</subject><subject>Toxins</subject><subject>二化螟</subject><subject>氨肽酶N</subject><subject>水稻螟虫</subject><subject>转Bt基因水稻</subject><subject>钙粘蛋白</subject><subject>鳞翅目昆虫</subject><issn>1672-9609</issn><issn>1744-7917</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqNkUFv1DAQhSMEEqXlN2Bx4UK24zgbxwcOsIJtpWoRKgVuI8eZ7HqbxKmdFdt_j7OpesYXj-zvzfg9JwnjsOBxXe4XXOZ5KhWXiwziKXAhxOL4Ijl7vngZ60JmqSpAvU7ehLAHECpT2VkS1tQTM63rbb9luq8ZHQdPIVjXM9cw3dneDTSMttaB2OaEGF3vyNueNd51rLP19jBO8Lgj5q0hFkbqWOU8-Y9stbOtY-EwnNrq1oaL5FWj20Bvn_bz5O7b15-rq_Tm-_p69fkmNXmZi1TWXIICyEoAzaUiDtIoKUFIQ1IJkqIQYFReq-hRSKhEpqqM8wpM0RCI8-TD3Hfw7uFAYcTOBkNtq3tyh4AlyKyMk8pIljNpvAvBU4ODt532j8gBp5hxj1OaOKWJU8x4ihmPUfpplv61LT3-tw6vN7dTFfXprLcxtOOzXvt7LKKpJf7erPHHnyv564socR35dzPfaId6623Au9vYWQAvVZ7xycz7JzM7128f4r9ipc19E9-HYrkEJcul-AetAqZu</recordid><startdate>201010</startdate><enddate>201010</enddate><creator>Yu, Hong-Kun</creator><creator>Chen, Hao</creator><creator>Zhang, Yong-Jun</creator><creator>Wu, Kong-Ming</creator><creator>Liang, Ge-Mei</creator><creator>Liu, Ze-Wen</creator><creator>Guo, Yu-Yuan</creator><general>Blackwell Publishing Asia</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>WU4</scope><scope>~WA</scope><scope>FBQ</scope><scope>BSCLL</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SS</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>201010</creationdate><title>Gene cloning and expression of aminopeptidase N and cadherin from midgut of the rice stem borer, Chilo suppressalis</title><author>Yu, Hong-Kun ; Chen, Hao ; Zhang, Yong-Jun ; Wu, Kong-Ming ; Liang, Ge-Mei ; Liu, Ze-Wen ; Guo, Yu-Yuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4843-7d1709002800a179e107c977037ce793e73630c94d9917370b329b211b0c6fe03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Amino acid sequence</topic><topic>Aminopeptidase</topic><topic>aminopeptidase N</topic><topic>Borers</topic><topic>Cadherin</topic><topic>Chilo suppressalis</topic><topic>CsCad</topic><topic>heterologous expression</topic><topic>Lepidoptera</topic><topic>membrane alanyl aminopeptidase</topic><topic>Midgut</topic><topic>Oryza sativa</topic><topic>Pest control</topic><topic>Pests</topic><topic>Tn cells</topic><topic>Toxins</topic><topic>二化螟</topic><topic>氨肽酶N</topic><topic>水稻螟虫</topic><topic>转Bt基因水稻</topic><topic>钙粘蛋白</topic><topic>鳞翅目昆虫</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yu, Hong-Kun</creatorcontrib><creatorcontrib>Chen, Hao</creatorcontrib><creatorcontrib>Zhang, Yong-Jun</creatorcontrib><creatorcontrib>Wu, Kong-Ming</creatorcontrib><creatorcontrib>Liang, Ge-Mei</creatorcontrib><creatorcontrib>Liu, Ze-Wen</creatorcontrib><creatorcontrib>Guo, Yu-Yuan</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库-自然科学-生物科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>AGRIS</collection><collection>Istex</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Insect science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yu, Hong-Kun</au><au>Chen, Hao</au><au>Zhang, Yong-Jun</au><au>Wu, Kong-Ming</au><au>Liang, Ge-Mei</au><au>Liu, Ze-Wen</au><au>Guo, Yu-Yuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gene cloning and expression of aminopeptidase N and cadherin from midgut of the rice stem borer, Chilo suppressalis</atitle><jtitle>Insect science</jtitle><addtitle>Insect Science</addtitle><date>2010-10</date><risdate>2010</risdate><volume>17</volume><issue>5</issue><spage>393</spage><epage>399</epage><pages>393-399</pages><issn>1672-9609</issn><eissn>1744-7917</eissn><abstract>The rice stem borer, Chilo suppressalis Walker is one of the most important insect pests on rice in Asia, north Africa and southern Europe. Transgenic Bt rice has been developed in the laboratory with good resistance to this pest and other Lepidopteran insects, which will provide a possible alternative tool for this pest control. The full-length cDNAs encoding an aminopeptidase N （CsAPN） and a cadherin （CsCad） were cloned from C. suppressalis. CsAPN showed common features of, and high identities to, other insect APNs in its deduced amino acid sequence. Although a full-length cDNA encoding cadherin-like protein has been reported in GenBank, the newly isolated cadherin here （CsCad） showed some differences in its amino acid sequence, especially at the 7th cadherin repeat region （CR7）, which indicated the newly isolated CsCad might be another allele. CsAPN and CsCad were successfully expressed in insect Tn cells, and the blot analysis showed these two proteins could bind Bt toxin CrylAb. The results will provide valuable information for the studies of toxin mode of action and the possible toxin resistance mechanisms in this pest.</abstract><cop>Melbourne, Australia</cop><pub>Blackwell Publishing Asia</pub><doi>10.1111/j.1744-7917.2010.01333.x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino acid sequence Aminopeptidase aminopeptidase N Borers Cadherin Chilo suppressalis CsCad heterologous expression Lepidoptera membrane alanyl aminopeptidase Midgut Oryza sativa Pest control Pests Tn cells Toxins 二化螟 氨肽酶N 水稻螟虫 转Bt基因水稻 钙粘蛋白 鳞翅目昆虫 |
| title | Gene cloning and expression of aminopeptidase N and cadherin from midgut of the rice stem borer, Chilo suppressalis |
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