Kinetics of asparaginase II fermentation in Saccharomyces cerevisiae ure2dal80 mutant: effect of nitrogen nutrition and pH
Although the quality of nitrogen source affects fermentation product formation, it has been managed empirically, to a large extent, in industrial scale. Laboratory-scale experiments successfully use the high-cost proline as a nonrepressive source. We evaluated urea as a substitute for proline in Sac...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2004, Vol.113-116 (1-3), p.299-306 |
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| container_title | Applied biochemistry and biotechnology |
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| creator | Ferrara, Maria Antonieta Mattoso, Josiane M V Bon, Elba P S Pereira, Jr, Nei |
| description | Although the quality of nitrogen source affects fermentation product formation, it has been managed empirically, to a large extent, in industrial scale. Laboratory-scale experiments successfully use the high-cost proline as a nonrepressive source. We evaluated urea as a substitute for proline in Saccharomyces cerevisiae ure2dal80 fermentations for asparaginase II production as a model system for nitrogen-regulated external enzymes. Maximum asparaginase II levels of 265 IU/L were observed in early stationary-phase cells grown on either proline or urea, whereas in ammonium cells, the maximum enzyme level was 157 IU/L. In all cases, enzyme stability was higher in buffered cultures with an initial pH of 6.5. |
| doi_str_mv | 10.1385/ABAB:113:1-3:299 |
| format | Article |
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Laboratory-scale experiments successfully use the high-cost proline as a nonrepressive source. We evaluated urea as a substitute for proline in Saccharomyces cerevisiae ure2dal80 fermentations for asparaginase II production as a model system for nitrogen-regulated external enzymes. Maximum asparaginase II levels of 265 IU/L were observed in early stationary-phase cells grown on either proline or urea, whereas in ammonium cells, the maximum enzyme level was 157 IU/L. 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| subjects | Ammonium Asparaginase - chemistry Asparaginase - genetics Biotechnology - methods Enzymes Fermentation Hydrogen-Ion Concentration Kinetics Mutation Nitrogen Nitrogen - chemistry Proline - chemistry Proteins Quaternary Ammonium Compounds - chemistry Saccharomyces cerevisiae Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Studies Time Factors Urea Urea - chemistry Yeast |
| title | Kinetics of asparaginase II fermentation in Saccharomyces cerevisiae ure2dal80 mutant: effect of nitrogen nutrition and pH |
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