Molecular characterization of nosRZDFYLX genes coding for denitrifying nitrous oxide reductase of Bradyrhizobium japonicum
The nosRZDFYLX gene cluster for the respiratory nitrous oxide reductase from Bradyrhizobium japonicum strain USDA110 has been cloned and sequenced. Seven protein coding regions corresponding to nosR, nosZ, the structural gene, nosD, nosF, nosY, nosL, and nosX were detected. The deduced amino acid se...
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| Veröffentlicht in: | Antonie van Leeuwenhoek 2004-04, Vol.85 (3), p.229-235 |
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| creator | VELASCO, Leonardo MESA, Socorro XU, Chang-Ai DELGADO, Maria J BEDMAR, Eulogio J |
| description | The nosRZDFYLX gene cluster for the respiratory nitrous oxide reductase from Bradyrhizobium japonicum strain USDA110 has been cloned and sequenced. Seven protein coding regions corresponding to nosR, nosZ, the structural gene, nosD, nosF, nosY, nosL, and nosX were detected. The deduced amino acid sequence exhibited a high degree of similarity to other nitrous oxide reductases from various sources. The NosZ protein included a signal peptide for protein export. Mutant strains carrying either a nosZ or a nosR mutation accumulated nitrous oxide when cultured microaerobically in the presence of nitrate. Maximal expression of a P nosZ-lacZ fusion in strain USDA110 required simultaneously both low level oxygen conditions and the presence of nitrate. Microaerobic activation of the fusion required FixLJ and FixK(2). |
| doi_str_mv | 10.1023/B:ANTO.0000020156.42470.db |
| format | Article |
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Seven protein coding regions corresponding to nosR, nosZ, the structural gene, nosD, nosF, nosY, nosL, and nosX were detected. The deduced amino acid sequence exhibited a high degree of similarity to other nitrous oxide reductases from various sources. The NosZ protein included a signal peptide for protein export. Mutant strains carrying either a nosZ or a nosR mutation accumulated nitrous oxide when cultured microaerobically in the presence of nitrate. Maximal expression of a P nosZ-lacZ fusion in strain USDA110 required simultaneously both low level oxygen conditions and the presence of nitrate. Microaerobic activation of the fusion required FixLJ and FixK(2).</description><identifier>ISSN: 0003-6072</identifier><identifier>EISSN: 1572-9699</identifier><identifier>DOI: 10.1023/B:ANTO.0000020156.42470.db</identifier><identifier>PMID: 15028871</identifier><identifier>CODEN: ANLEDR</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Amino Acid Sequence ; Amino acids ; Bacteria ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bacteriology ; Biological and medical sciences ; Bradyrhizobium - enzymology ; Bradyrhizobium - genetics ; Bradyrhizobium japonicum ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Bacterial ; Genes, Bacterial ; Genes, Reporter ; Genetics ; Membrane Proteins - genetics ; Membrane Proteins - metabolism ; Microbiology ; Molecular Sequence Data ; Nitrous oxide ; Nitrous Oxide - metabolism ; Oxidoreductases - genetics ; Oxidoreductases - metabolism ; Promoter Regions, Genetic ; Proteins ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - metabolism</subject><ispartof>Antonie van Leeuwenhoek, 2004-04, Vol.85 (3), p.229-235</ispartof><rights>Kluwer Academic Publishers 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c461t-ea70577994eecc169f99b0146ada76665ca9210e2220e3d0cd82588da6e790ab3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16398333$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15028871$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>VELASCO, Leonardo</creatorcontrib><creatorcontrib>MESA, Socorro</creatorcontrib><creatorcontrib>XU, Chang-Ai</creatorcontrib><creatorcontrib>DELGADO, Maria J</creatorcontrib><creatorcontrib>BEDMAR, Eulogio J</creatorcontrib><title>Molecular characterization of nosRZDFYLX genes coding for denitrifying nitrous oxide reductase of Bradyrhizobium japonicum</title><title>Antonie van Leeuwenhoek</title><addtitle>Antonie Van Leeuwenhoek</addtitle><description>The nosRZDFYLX gene cluster for the respiratory nitrous oxide reductase from Bradyrhizobium japonicum strain USDA110 has been cloned and sequenced. Seven protein coding regions corresponding to nosR, nosZ, the structural gene, nosD, nosF, nosY, nosL, and nosX were detected. The deduced amino acid sequence exhibited a high degree of similarity to other nitrous oxide reductases from various sources. The NosZ protein included a signal peptide for protein export. Mutant strains carrying either a nosZ or a nosR mutation accumulated nitrous oxide when cultured microaerobically in the presence of nitrate. Maximal expression of a P nosZ-lacZ fusion in strain USDA110 required simultaneously both low level oxygen conditions and the presence of nitrate. Microaerobic activation of the fusion required FixLJ and FixK(2).</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Bacteria</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Bradyrhizobium - enzymology</subject><subject>Bradyrhizobium - genetics</subject><subject>Bradyrhizobium japonicum</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genes, Bacterial</subject><subject>Genes, Reporter</subject><subject>Genetics</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - metabolism</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Nitrous oxide</subject><subject>Nitrous Oxide - metabolism</subject><subject>Oxidoreductases - genetics</subject><subject>Oxidoreductases - metabolism</subject><subject>Promoter Regions, Genetic</subject><subject>Proteins</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><issn>0003-6072</issn><issn>1572-9699</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kU1v1DAQQC0EotvCX0BWJeCUxXYSO-6tWyggLVRCRQIulmNPWq8Se7ETqbu_HoeutBIH5uKvN-OxH0LnlCwpYeW71cXl19ubJZmDEVrzZcUqQZa2fYIWtBaskFzKp2iRz8uCE8FO0GlKm7yUvBHP0QmtCWsaQRdo_yX0YKZeR2zuddRmhOj2enTB49BhH9K3X--vf65_4DvwkLAJ1vk73IWILXg3Rtft5o15GqaEw4OzgCPYyYw6wVxjFbXdxXu3D62bBrzR2-CdmYYX6Fmn-wQvD-MZ-n794fbqU7G--fj56nJdmIrTsQAtSC2ElBWAMZTLTsqW0IprqwXnvDZaMkqAMUagtMTYhtVNYzUHIYluyzP09rHuNobfE6RRDS4Z6HvtIbesmvxBTUklz-Sb_5KCZrKqWAbP_wE3YYo-v0KJuqxqmcEMXTxCJoaUInRqG92g405RomaRaqVmkeooUv0Vqezc9KvDDVM7gD2mHsxl4PUB0MnovovaG5eOHC9lU-b4AwnpqPU</recordid><startdate>20040401</startdate><enddate>20040401</enddate><creator>VELASCO, Leonardo</creator><creator>MESA, Socorro</creator><creator>XU, Chang-Ai</creator><creator>DELGADO, Maria J</creator><creator>BEDMAR, Eulogio J</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20040401</creationdate><title>Molecular characterization of nosRZDFYLX genes coding for denitrifying nitrous oxide reductase of Bradyrhizobium japonicum</title><author>VELASCO, Leonardo ; MESA, Socorro ; XU, Chang-Ai ; DELGADO, Maria J ; BEDMAR, Eulogio J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c461t-ea70577994eecc169f99b0146ada76665ca9210e2220e3d0cd82588da6e790ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Bacteria</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Bradyrhizobium - enzymology</topic><topic>Bradyrhizobium - genetics</topic><topic>Bradyrhizobium japonicum</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genes, Bacterial</topic><topic>Genes, Reporter</topic><topic>Genetics</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - metabolism</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Nitrous oxide</topic><topic>Nitrous Oxide - metabolism</topic><topic>Oxidoreductases - genetics</topic><topic>Oxidoreductases - metabolism</topic><topic>Promoter Regions, Genetic</topic><topic>Proteins</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>VELASCO, Leonardo</creatorcontrib><creatorcontrib>MESA, Socorro</creatorcontrib><creatorcontrib>XU, Chang-Ai</creatorcontrib><creatorcontrib>DELGADO, Maria J</creatorcontrib><creatorcontrib>BEDMAR, Eulogio J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Antonie van Leeuwenhoek</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>VELASCO, Leonardo</au><au>MESA, Socorro</au><au>XU, Chang-Ai</au><au>DELGADO, Maria J</au><au>BEDMAR, Eulogio J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterization of nosRZDFYLX genes coding for denitrifying nitrous oxide reductase of Bradyrhizobium japonicum</atitle><jtitle>Antonie van Leeuwenhoek</jtitle><addtitle>Antonie Van Leeuwenhoek</addtitle><date>2004-04-01</date><risdate>2004</risdate><volume>85</volume><issue>3</issue><spage>229</spage><epage>235</epage><pages>229-235</pages><issn>0003-6072</issn><eissn>1572-9699</eissn><coden>ANLEDR</coden><abstract>The nosRZDFYLX gene cluster for the respiratory nitrous oxide reductase from Bradyrhizobium japonicum strain USDA110 has been cloned and sequenced. Seven protein coding regions corresponding to nosR, nosZ, the structural gene, nosD, nosF, nosY, nosL, and nosX were detected. The deduced amino acid sequence exhibited a high degree of similarity to other nitrous oxide reductases from various sources. The NosZ protein included a signal peptide for protein export. Mutant strains carrying either a nosZ or a nosR mutation accumulated nitrous oxide when cultured microaerobically in the presence of nitrate. Maximal expression of a P nosZ-lacZ fusion in strain USDA110 required simultaneously both low level oxygen conditions and the presence of nitrate. Microaerobic activation of the fusion required FixLJ and FixK(2).</abstract><cop>Dordrecht</cop><pub>Springer</pub><pmid>15028871</pmid><doi>10.1023/B:ANTO.0000020156.42470.db</doi><tpages>7</tpages></addata></record> |
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| subjects | Amino Acid Sequence Amino acids Bacteria Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacteriology Biological and medical sciences Bradyrhizobium - enzymology Bradyrhizobium - genetics Bradyrhizobium japonicum Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial Genes, Bacterial Genes, Reporter Genetics Membrane Proteins - genetics Membrane Proteins - metabolism Microbiology Molecular Sequence Data Nitrous oxide Nitrous Oxide - metabolism Oxidoreductases - genetics Oxidoreductases - metabolism Promoter Regions, Genetic Proteins Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism |
| title | Molecular characterization of nosRZDFYLX genes coding for denitrifying nitrous oxide reductase of Bradyrhizobium japonicum |
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