Growth and starvation of a strain of Klebsiella pneumoniae isolated from a Brazilian oil formation
The changes that occur in cell size and shape during experimental starvation and resuscitation of bacteria may find practical application in the biotechnological processes used in microbial enhanced oil-recovery (MEOR), in insitu bioremediation and in the creation of biobarriers. In the work describ...
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| Veröffentlicht in: | World journal of microbiology & biotechnology 2005-12, Vol.21 (8-9), p.1471-1475 |
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| container_issue | 8-9 |
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| container_title | World journal of microbiology & biotechnology |
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| creator | SEGNINI BOSSOLAN, Nelma Reeina LEAL GODINHO, Mirna Januaria GARCIA TORRES VOLPON, Antonia |
| description | The changes that occur in cell size and shape during experimental starvation and resuscitation of bacteria may find practical application in the biotechnological processes used in microbial enhanced oil-recovery (MEOR), in insitu bioremediation and in the creation of biobarriers. In the work described here, the aim was to observe certain aspects of the response of a strain of Klebsiella pneumoniae, isolated from oil producer wells belonging to PETROBRAS (National Oil Company of Brazil), to starvation and growth under various culture conditions. Cells of K.pneumoniae were taken from an exponentially-growing culture, washed and suspended in phosphate-buffered saline solution, without nutrients, where they remained for 91days. Aliquots were withdrawn periodically, to perform viable and total cell counts and measure the optical density and cell dimensions. The starved cells were resuspended in sodium citrate medium, where they recuperated and exhibited a typical growth curve. The results indicate that this strain is a viable option for future trials on the transport and growth of microorganisms inside porous media, aimed at possible applications in MEOR. |
| doi_str_mv | 10.1007/s11274-005-6811-7 |
| format | Article |
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In the work described here, the aim was to observe certain aspects of the response of a strain of Klebsiella pneumoniae, isolated from oil producer wells belonging to PETROBRAS (National Oil Company of Brazil), to starvation and growth under various culture conditions. Cells of K.pneumoniae were taken from an exponentially-growing culture, washed and suspended in phosphate-buffered saline solution, without nutrients, where they remained for 91days. Aliquots were withdrawn periodically, to perform viable and total cell counts and measure the optical density and cell dimensions. The starved cells were resuspended in sodium citrate medium, where they recuperated and exhibited a typical growth curve. 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In the work described here, the aim was to observe certain aspects of the response of a strain of Klebsiella pneumoniae, isolated from oil producer wells belonging to PETROBRAS (National Oil Company of Brazil), to starvation and growth under various culture conditions. Cells of K.pneumoniae were taken from an exponentially-growing culture, washed and suspended in phosphate-buffered saline solution, without nutrients, where they remained for 91days. Aliquots were withdrawn periodically, to perform viable and total cell counts and measure the optical density and cell dimensions. The starved cells were resuspended in sodium citrate medium, where they recuperated and exhibited a typical growth curve. 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that occur in cell size and shape during experimental starvation and resuscitation of bacteria may find practical application in the biotechnological processes used in microbial enhanced oil-recovery (MEOR), in insitu bioremediation and in the creation of biobarriers. In the work described here, the aim was to observe certain aspects of the response of a strain of Klebsiella pneumoniae, isolated from oil producer wells belonging to PETROBRAS (National Oil Company of Brazil), to starvation and growth under various culture conditions. Cells of K.pneumoniae were taken from an exponentially-growing culture, washed and suspended in phosphate-buffered saline solution, without nutrients, where they remained for 91days. Aliquots were withdrawn periodically, to perform viable and total cell counts and measure the optical density and cell dimensions. The starved cells were resuspended in sodium citrate medium, where they recuperated and exhibited a typical growth curve. 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| identifier | ISSN: 0959-3993 |
| ispartof | World journal of microbiology & biotechnology, 2005-12, Vol.21 (8-9), p.1471-1475 |
| issn | 0959-3993 1573-0972 |
| language | eng |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Bacteria Biological and medical sciences Bioremediation Biotechnology Brazil Culture Fundamental and applied biological sciences. Psychology Klebsiella Klebsiella pneumoniae Microorganisms Porous media Resuscitation Sodium Strain |
| title | Growth and starvation of a strain of Klebsiella pneumoniae isolated from a Brazilian oil formation |
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