Agrobacterium tumefaciens-mediated transformation ofMucor circinelloides
TheAgrobacterium tumefaciens-mediated transformation of the zygomycetous fungusMucor circinelloides is described. A method was also developed for the hygromycin B-based selection ofMucor transformants. Transformation with the hygromycin B phosphotransferase gene ofEscherichia coli controlled by the...
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| Veröffentlicht in: | Folia microbiologica 2005-09, Vol.50 (5), p.415-420, Article 415 |
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| creator | Nyilasi, I Ács, K Papp, T Nagy, E Vágvölgyi, C |
| description | TheAgrobacterium tumefaciens-mediated transformation of the zygomycetous fungusMucor circinelloides is described. A method was also developed for the hygromycin B-based selection ofMucor transformants. Transformation with the hygromycin B phosphotransferase gene ofEscherichia coli controlled by the heterologousAspergillus nidulans trpC promoter resulted in hygromycin B-resistant clones. The presence of the hygromycin resistance gene in the genome of the transformants was verified by polymerase chain reaction and Southern hybridization: the latter analyses revealed integrations in the host genome at different sites in different transformants. The stability of transformants remained questionable during the latter analyses. |
| doi_str_mv | 10.1007/BF02931423 |
| format | Article |
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| subjects | Agrobacterium Genes Genetic transformation Genetics Genomes Hybridization Hygromycin Hygromycin B Phosphotransferase Phosphotransferase gene Polymerase chain reaction Stability analysis Transformations Transient receptor potential proteins |
| title | Agrobacterium tumefaciens-mediated transformation ofMucor circinelloides |
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